Open Access
Science, volume 370, issue 6523, pages 1479-1484
Versatile and multivalent nanobodies efficiently neutralize SARS-CoV-2
Yufei Xiang
1
,
Sham Nambulli
2, 3
,
Zhengyun Xiao
1
,
Heng Liu
4
,
Zhe Sang
1, 5
,
W. Paul Duprex
2, 3
,
Dina Schneidman-Duhovny
6
,
Cheng Zhang
4
,
Yi Shi
1, 5
1
Department of Cell Biology, University of Pittsburgh, Pittsburgh, PA, USA.
|
2
Center for Vaccine Research, University of Pittsburgh, Pittsburgh, PA, USA.
|
3
Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, PA, USA.
|
4
Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA, USA.
|
Publication type: Journal Article
Publication date: 2020-12-18
Multidisciplinary
Abstract
Nanobodies that neutralize
Monoclonal antibodies that bind to the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) show therapeutic promise but must be produced in mammalian cells and need to be delivered intravenously. By contrast, single-domain antibodies called nanobodies can be produced in bacteria or yeast, and their stability may enable aerosol delivery. Two papers now report nanobodies that bind tightly to spike and efficiently neutralize SARS-CoV-2 in cells. Schoof et al. screened a yeast surface display of synthetic nanobodies and Xiang et al. screened anti-spike nanobodies produced by a llama. Both groups identified highly potent nanobodies that lock the spike protein in an inactive conformation. Multivalent constructs of selected nanobodies achieved even more potent neutralization.
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Xiang Y. et al. Versatile and multivalent nanobodies efficiently neutralize SARS-CoV-2 // Science. 2020. Vol. 370. No. 6523. pp. 1479-1484.
GOST all authors (up to 50)
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Xiang Y., Nambulli S., Xiao Z., Liu H., Sang Z., Duprex W. P., Schneidman-Duhovny D., Zhang C., Shi Y. Versatile and multivalent nanobodies efficiently neutralize SARS-CoV-2 // Science. 2020. Vol. 370. No. 6523. pp. 1479-1484.
Cite this
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TY - JOUR
DO - 10.1126/science.abe4747
UR - https://doi.org/10.1126/science.abe4747
TI - Versatile and multivalent nanobodies efficiently neutralize SARS-CoV-2
T2 - Science
AU - Xiang, Yufei
AU - Nambulli, Sham
AU - Xiao, Zhengyun
AU - Liu, Heng
AU - Sang, Zhe
AU - Duprex, W. Paul
AU - Schneidman-Duhovny, Dina
AU - Zhang, Cheng
AU - Shi, Yi
PY - 2020
DA - 2020/12/18
PB - American Association for the Advancement of Science (AAAS)
SP - 1479-1484
IS - 6523
VL - 370
SN - 0036-8075
SN - 1095-9203
ER -
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BibTex (up to 50 authors)
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@article{2020_Xiang,
author = {Yufei Xiang and Sham Nambulli and Zhengyun Xiao and Heng Liu and Zhe Sang and W. Paul Duprex and Dina Schneidman-Duhovny and Cheng Zhang and Yi Shi},
title = {Versatile and multivalent nanobodies efficiently neutralize SARS-CoV-2},
journal = {Science},
year = {2020},
volume = {370},
publisher = {American Association for the Advancement of Science (AAAS)},
month = {dec},
url = {https://doi.org/10.1126/science.abe4747},
number = {6523},
pages = {1479--1484},
doi = {10.1126/science.abe4747}
}
Cite this
MLA
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Xiang, Yufei, et al. “Versatile and multivalent nanobodies efficiently neutralize SARS-CoV-2.” Science, vol. 370, no. 6523, Dec. 2020, pp. 1479-1484. https://doi.org/10.1126/science.abe4747.