Open Access

Science

, volume 370 , issue 6523 , pages 1479-1484

Versatile and multivalent nanobodies efficiently neutralize SARS-CoV-2

Yufei Xiang ¹

Sham Nambulli ^{2, 3}

Zhengyun Xiao ¹

Heng Liu ⁴

Zhe Sang ^{1, 5}

W. Paul Duprex ^{2, 3}

Dina Schneidman-Duhovny ⁶

Cheng Zhang ⁴

Yi Shi ^{1, 5}

Hide authors affiliations Show authors affiliations: 6 affiliations

Department of Cell Biology, University of Pittsburgh, Pittsburgh, PA, USA. |

Center for Vaccine Research, University of Pittsburgh, Pittsburgh, PA, USA. |

Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, PA, USA. |

⁴

Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA, USA. |

⁵

University of Pittsburgh–Carnegie Mellon University Program in Computational Biology, Pittsburgh, PA, USA. |

⁶

School of Computer Science and Engineering, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel. |

Publication type: Journal Article

Publication date: 2020-12-18

American Association for the Advancement of Science (AAAS)

Science

scimago Q1

wos Q1

SJR: 10.416

CiteScore: 48.4

Impact factor: 45.8

ISSN: 00368075, 10959203

DOI: 10.1126/science.abe4747

Copy DOI

PubMed ID: 33154108

Multidisciplinary

Abstract

Nanobodies that neutralize

Monoclonal antibodies that bind to the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) show therapeutic promise but must be produced in mammalian cells and need to be delivered intravenously. By contrast, single-domain antibodies called nanobodies can be produced in bacteria or yeast, and their stability may enable aerosol delivery. Two papers now report nanobodies that bind tightly to spike and efficiently neutralize SARS-CoV-2 in cells. Schoof et al. screened a yeast surface display of synthetic nanobodies and Xiang et al. screened anti-spike nanobodies produced by a llama. Both groups identified highly potent nanobodies that lock the spike protein in an inactive conformation. Multivalent constructs of selected nanobodies achieved even more potent neutralization.

Science , this issue p. 1473 , p. 1479

Found

3 citations

Dolzhikova Inna

🥼

PhD in Biological/biomedical sciences

54 publications, 2 910 citations

h-index: 15

N. F. Gamaleya National Research Center for Epidemiology and Microbiology of the Ministry of Health of the Russian Federation

D. I. Ivanovsky Institute of Virology

RNA drugs and vaccines

Virology

1 citation

Alekseev Alexander

74 publications, 650 citations, 7 reviews

h-index: 14

Dagestan State University

Federal Research Center of Fundamental and Translational Medicine

1 citation

Sluchanko Nikolai

🥼 🤝

DSc in Biological/biomedical sciences

155 publications, 2 871 citations, 73 reviews

h-index: 33

Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences

N. F. Gamaleya National Research Center for Epidemiology and Microbiology of the Ministry of Health of the Russian Federation

Research interests

Protein Structure

Protein-protein interactions

Structural Biology

1 citation

Sinitcyn Pavel

28 publications, 9 474 citations, 9 reviews

h-index: 15

Max Planck Institute of Biochemistry

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GOST Copy

Xiang Y. et al. Versatile and multivalent nanobodies efficiently neutralize SARS-CoV-2 // Science. 2020. Vol. 370. No. 6523. pp. 1479-1484.

GOST all authors (up to 50) Copy

Xiang Y., Nambulli S., Xiao Z., Liu H., Sang Z., Duprex W. P., Schneidman-Duhovny D., Zhang C., Shi Y. Versatile and multivalent nanobodies efficiently neutralize SARS-CoV-2 // Science. 2020. Vol. 370. No. 6523. pp. 1479-1484.

RIS |

Cite this

RIS Copy

TY - JOUR

DO - 10.1126/science.abe4747

UR - https://doi.org/10.1126/science.abe4747

TI - Versatile and multivalent nanobodies efficiently neutralize SARS-CoV-2

T2 - Science

AU - Xiang, Yufei

AU - Nambulli, Sham

AU - Xiao, Zhengyun

AU - Liu, Heng

AU - Sang, Zhe

AU - Duprex, W. Paul

AU - Schneidman-Duhovny, Dina

AU - Zhang, Cheng

AU - Shi, Yi

PY - 2020

DA - 2020/12/18

PB - American Association for the Advancement of Science (AAAS)

SP - 1479-1484

IS - 6523

VL - 370

PMID - 33154108

SN - 0036-8075

SN - 1095-9203

ER -

BibTex |

Cite this

BibTex (up to 50 authors) Copy

@article{2020_Xiang,

author = {Yufei Xiang and Sham Nambulli and Zhengyun Xiao and Heng Liu and Zhe Sang and W. Paul Duprex and Dina Schneidman-Duhovny and Cheng Zhang and Yi Shi},

title = {Versatile and multivalent nanobodies efficiently neutralize SARS-CoV-2},

journal = {Science},

year = {2020},

volume = {370},

publisher = {American Association for the Advancement of Science (AAAS)},

month = {dec},

url = {https://doi.org/10.1126/science.abe4747},

number = {6523},

pages = {1479--1484},

doi = {10.1126/science.abe4747}

}

MLA

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MLA Copy

Xiang, Yufei, et al. “Versatile and multivalent nanobodies efficiently neutralize SARS-CoV-2.” Science, vol. 370, no. 6523, Dec. 2020, pp. 1479-1484. https://doi.org/10.1126/science.abe4747.

Publisher

American Association for the Advancement of Science (AAAS)

Journal

Science

scimago Q1

wos Q1

SJR

10.416

CiteScore

48.4

Impact factor

45.8

ISSN

00368075 (Print)

10959203 (Electronic)