Biochemistry (Moscow), volume 86, issue 9, pages 1128-1138
VPg of Potato Virus Y and Potato Cap-Binding eIF4E Factors: Selective Interaction and Its Supposed Mechanism
Publication type: Journal Article
Publication date: 2021-09-13
Journal:
Biochemistry (Moscow)
Quartile SCImago
Q2
Quartile WOS
Q3
Impact factor: 2.8
ISSN: 00062979, 16083040
Biochemistry
General Medicine
Abstract
Potato virus Y (PVY) is one of the most common and harmful plant viruses. Translation of viral RNA starts with the interaction between the plant cap-binding translation initiation factors eIF4E and viral genome-linked protein (VPg) covalently attached to the viral RNA. Disruption of this interaction is one of the natural mechanisms of plant resistance to PVY. The multigene eIF4E family in the potato (Solanum tuberosum L.) genome contains genes for the translation initiation factors eIF4E1, eIF4E2, and eIF(iso)4E. However, which of these factors can be recruited by the PVY, as well as the mechanism of this interaction, remain obscure. Here, we showed that the most common VPg variant from the PVY strain NTN interacts with eIF4E1 and eIF4E2, but not with eIF(iso)4E. Based on the VPg, eIF4E1, and eIF4E2 models and data on the natural polymorphism of VPg amino acid sequence, we suggested that the key role in the recognition of potato cap-binding factors belongs to the R104 residue of VPg. To verify this hypothesis, we created VPg mutants with substitutions at position 104 and examined their ability to interact with potato eIF4E factors. The obtained data were used to build the theoretical model of the VPg-eIF4E2 complex that differs significantly from the earlier models of VPg complexes with eIF4E proteins, but is in a good agreement with the current biochemical data.
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Lebedeva M. V. et al. VPg of Potato Virus Y and Potato Cap-Binding eIF4E Factors: Selective Interaction and Its Supposed Mechanism // Biochemistry (Moscow). 2021. Vol. 86. No. 9. pp. 1128-1138.
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Lebedeva M. V., Nikonova E. Y., Terentiev A. A., Taranov V. V., Babakov A. V., Nikonov O. S. VPg of Potato Virus Y and Potato Cap-Binding eIF4E Factors: Selective Interaction and Its Supposed Mechanism // Biochemistry (Moscow). 2021. Vol. 86. No. 9. pp. 1128-1138.
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TY - JOUR
DO - 10.1134/s000629792109008x
UR - https://doi.org/10.1134%2Fs000629792109008x
TI - VPg of Potato Virus Y and Potato Cap-Binding eIF4E Factors: Selective Interaction and Its Supposed Mechanism
T2 - Biochemistry (Moscow)
AU - Lebedeva, Marina V.
AU - Nikonova, Ekaterina Y.
AU - Terentiev, Alexey A
AU - Taranov, Vasiliy V
AU - Babakov, Alexey V
AU - Nikonov, Oleg S
PY - 2021
DA - 2021/09/13 00:00:00
PB - Pleiades Publishing
SP - 1128-1138
IS - 9
VL - 86
SN - 0006-2979
SN - 1608-3040
ER -
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@article{2021_Lebedeva,
author = {Marina V. Lebedeva and Ekaterina Y. Nikonova and Alexey A Terentiev and Vasiliy V Taranov and Alexey V Babakov and Oleg S Nikonov},
title = {VPg of Potato Virus Y and Potato Cap-Binding eIF4E Factors: Selective Interaction and Its Supposed Mechanism},
journal = {Biochemistry (Moscow)},
year = {2021},
volume = {86},
publisher = {Pleiades Publishing},
month = {sep},
url = {https://doi.org/10.1134%2Fs000629792109008x},
number = {9},
pages = {1128--1138},
doi = {10.1134/s000629792109008x}
}
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Lebedeva, Marina V., et al. “VPg of Potato Virus Y and Potato Cap-Binding eIF4E Factors: Selective Interaction and Its Supposed Mechanism.” Biochemistry (Moscow), vol. 86, no. 9, Sep. 2021, pp. 1128-1138. https://doi.org/10.1134%2Fs000629792109008x.