Molecular Biology, volume 56, issue 6, pages 1028-1035
High Heterogeneity of Virus-Neutralizing and RBD-Binding Activities of COVID-19 Convalescent Sera
E A Astakhova
1, 2
,
M G Byazrova
1, 2, 3
,
G M Yusubalieva
4
,
V F Larichev
5
,
V P Baklaushev
4
,
A. V. Filatov
1, 2
Publication type: Journal Article
Publication date: 2022-12-09
Journal:
Molecular Biology
Quartile SCImago
Q4
Quartile WOS
Q4
Impact factor: 1.2
ISSN: 00268933, 16083245, 21689547
PubMed ID:
36532266
Structural Biology
Biophysics
Abstract
The parameters of the humoral response are an important immunological characteristic of donors who recovered from COVID-19 and vaccinated individuals. Analysis of the level of virus-binding antibodies has become widespread. The most accurate predictor of effective immune protection against symptomatic SARS-CoV-2 infection is the activity of virus-neutralizing antibodies. We determined virus-neutralizing activities in plasma samples of individuals (n = 111) who had COVID-19 from April to September 2020. Three independent methods were used: conventional with live virus, with virus-like particles pseudotyped with spike protein, and a surrogate virus-neutralization test (cVNT, pVNT and sVNT, respectively). For comparison, the levels of IgG, IgA and IgM antibodies against the receptor-binding domain of the SARS-CoV-2 spike protein were also evaluated. The levels of virus-binding as well as virus-neutralizing antibodies in cVNT and pVNT showed high heterogeneity. A comparison of cVNT and pVNT results showed a high correlation, sVNT results also correlated well with both cVNT and pVNT. To the greatest extent, the level of IgG antibodies correlated with the results of cVNT, pVNT and sVNT. These results can be used in the selection of plasmas that are best suited for transfusion and treatment of acute COVID-19. In addition, data on the virus-neutralizing activity of plasma are important for the selection of potential donors, for the isolation of SARS-CoV-2-specific B-lymphocytes, in order to further generate monoclonal virus-neutralizing antibodies.
Top-30
Citations by journals
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1
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International Journal of Molecular Sciences
1 publication, 33.33%
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Biochemistry (Moscow)
1 publication, 33.33%
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Russian Chemical Reviews
1 publication, 33.33%
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1
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Citations by publishers
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1
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Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 33.33%
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Pleiades Publishing
1 publication, 33.33%
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Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
1 publication, 33.33%
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1
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Astakhova E. A. et al. High Heterogeneity of Virus-Neutralizing and RBD-Binding Activities of COVID-19 Convalescent Sera // Molecular Biology. 2022. Vol. 56. No. 6. pp. 1028-1035.
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Astakhova E. A., Byazrova M. G., Yusubalieva G. M., Larichev V. F., Baklaushev V. P., Filatov A. V. High Heterogeneity of Virus-Neutralizing and RBD-Binding Activities of COVID-19 Convalescent Sera // Molecular Biology. 2022. Vol. 56. No. 6. pp. 1028-1035.
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TY - JOUR
DO - 10.1134/s002689332206005x
UR - https://doi.org/10.1134/s002689332206005x
TI - High Heterogeneity of Virus-Neutralizing and RBD-Binding Activities of COVID-19 Convalescent Sera
T2 - Molecular Biology
AU - Astakhova, E A
AU - Byazrova, M G
AU - Yusubalieva, G M
AU - Larichev, V F
AU - Baklaushev, V P
AU - Filatov, A. V.
PY - 2022
DA - 2022/12/09 00:00:00
PB - Pleiades Publishing
SP - 1028-1035
IS - 6
VL - 56
PMID - 36532266
SN - 0026-8933
SN - 1608-3245
SN - 2168-9547
ER -
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@article{2022_Astakhova,
author = {E A Astakhova and M G Byazrova and G M Yusubalieva and V F Larichev and V P Baklaushev and A. V. Filatov},
title = {High Heterogeneity of Virus-Neutralizing and RBD-Binding Activities of COVID-19 Convalescent Sera},
journal = {Molecular Biology},
year = {2022},
volume = {56},
publisher = {Pleiades Publishing},
month = {dec},
url = {https://doi.org/10.1134/s002689332206005x},
number = {6},
pages = {1028--1035},
doi = {10.1134/s002689332206005x}
}
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Astakhova, E. A., et al. “High Heterogeneity of Virus-Neutralizing and RBD-Binding Activities of COVID-19 Convalescent Sera.” Molecular Biology, vol. 56, no. 6, Dec. 2022, pp. 1028-1035. https://doi.org/10.1134/s002689332206005x.