Open Access
Yeast Debaryomyces hansenii within ORMOSIL Shells as a Heterogeneous Biocatalyst
O N Ponamoreva
1
,
E L Afonina
1
,
O A Kamanina
1
,
D G Lavrova
1
,
V. A. Arlyapov
1
,
V A Alferov
1
,
A. M. Boronin
2
Тип публикации: Journal Article
Дата публикации: 2018-12-01
scimago Q3
wos Q4
БС2
SJR: 0.294
CiteScore: 1.7
Impact factor: 1.1
ISSN: 00036838, 16083024
Biochemistry
Applied Microbiology and Biotechnology
Краткое описание
The relevant sol-gel encapsulation methods to design ORMOSIL (organically modified silica) protective shells around Debaryomyces hansenii yeast cells have been studied. Encapsulation is based on spontaneous “cell-in-shell” 3D structures. The physiological activity of the sol-gel encapsulated cells was determined by measurement of the oxidative activity of a BOD biosensor containing ORMOSIL-encapsulated cells. It was shown that the shells around the microbial cells did not prevent substrate diffusion and had excellent protection from extreme environmental factors (heavy metal ions, high pH, and UV radiation). In addition, it was found that the BOD biosensor surpasses some other known biosensors in their basic characteristics, including sensitivity, stability, and reproducibility. These results are important for the development of innovative methods of cell encapsulation and the creation of biocatalysts used in biotechnology and ecology.
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ГОСТ
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Ponamoreva O. N. et al. Yeast Debaryomyces hansenii within ORMOSIL Shells as a Heterogeneous Biocatalyst // Applied Biochemistry and Microbiology. 2018. Vol. 54. No. 7. pp. 736-742.
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Ponamoreva O. N., Afonina E. L., Kamanina O. A., Lavrova D. G., Arlyapov V. A., Alferov V. A., Boronin A. M. Yeast Debaryomyces hansenii within ORMOSIL Shells as a Heterogeneous Biocatalyst // Applied Biochemistry and Microbiology. 2018. Vol. 54. No. 7. pp. 736-742.
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TY - JOUR
DO - 10.1134/S0003683818070062
UR - https://doi.org/10.1134/S0003683818070062
TI - Yeast Debaryomyces hansenii within ORMOSIL Shells as a Heterogeneous Biocatalyst
T2 - Applied Biochemistry and Microbiology
AU - Ponamoreva, O N
AU - Afonina, E L
AU - Kamanina, O A
AU - Lavrova, D G
AU - Arlyapov, V. A.
AU - Alferov, V A
AU - Boronin, A. M.
PY - 2018
DA - 2018/12/01
PB - Pleiades Publishing
SP - 736-742
IS - 7
VL - 54
SN - 0003-6838
SN - 1608-3024
ER -
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BibTex (до 50 авторов)
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@article{2018_Ponamoreva,
author = {O N Ponamoreva and E L Afonina and O A Kamanina and D G Lavrova and V. A. Arlyapov and V A Alferov and A. M. Boronin},
title = {Yeast Debaryomyces hansenii within ORMOSIL Shells as a Heterogeneous Biocatalyst},
journal = {Applied Biochemistry and Microbiology},
year = {2018},
volume = {54},
publisher = {Pleiades Publishing},
month = {dec},
url = {https://doi.org/10.1134/S0003683818070062},
number = {7},
pages = {736--742},
doi = {10.1134/S0003683818070062}
}
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MLA
Скопировать
Ponamoreva, O. N., et al. “Yeast Debaryomyces hansenii within ORMOSIL Shells as a Heterogeneous Biocatalyst.” Applied Biochemistry and Microbiology, vol. 54, no. 7, Dec. 2018, pp. 736-742. https://doi.org/10.1134/S0003683818070062.