Biochemistry (Moscow), volume 86, issue 8, pages 1012-1024
RNA Aptamers for Theranostics of Glioblastoma of Human Brain
Kopylov Alexey M
1
,
Fab Lika V
2
,
Antipova Olga
1
,
Savchenko Ekaterina A
3
,
Revishchin Alexander V
2
,
Parshina Viktoriya V
2
,
Pavlova Svetlana V
2
,
Kireev Igor I
1
,
Golovin Andrey V.
1, 4
,
Usachev Dmitry Y
3
,
Pavlova Galina V
2, 3, 4
Publication type: Journal Article
Publication date: 2021-08-13
Journal:
Biochemistry (Moscow)
Quartile SCImago
Q2
Quartile WOS
Q3
Impact factor: 2.8
ISSN: 00062979, 16083040
Biochemistry
General Medicine
Abstract
Abstract Conventional approaches for studying and molecular typing of tumors include PCR, blotting, omics, immunocytochemistry, and immunohistochemistry. The last two methods are the most used, as they enable detecting both tumor protein markers and their localizations within the cells. In this study, we have investigated a possibility of using RNA aptamers, in particular, 2′-F-pyrimidyl-RNA aptamer ME07 (48 nucleotides long), specific to the receptor of epidermal growth factor (EGFR, ErbB1, Her1), as an alternative to monoclonal antibodies for aptacytochemistry and aptahistochemistry for human glioblastoma multiforme (GBM). A specificity of binding of FAM-ME07 to the receptor on the tumor cells has been demonstrated by flow cytometry; an apparent dissociation constant for the complex of aptamer – EGFR on the cell has been determined; a number of EGFR molecules has been semi-quantitatively estimated for the tumor cell lines having different amount of EGFR: A431 (10 6 copies per cell), U87 (10 4 copies per cell), MCF7 (10 3 copies per cell), and ROZH, primary GBM cell culture derived from patient (10 4 copies per cell). According to fluorescence microscopy, FAM-ME07 interacts directly with the receptors on A431 cells, followed by its internalization into the cytoplasm and translocation to the nucleolus; this finding opens a possibility of ME07 application as an escort aptamer for a delivery of therapeutic agents into tumor cells. FAM-ME07 efficiently stains sections of GBM clinical specimens, which enables an identification of EGFR-positive clones within a heterogeneous tumor; and providing a potential for further studying animal models of GBM.
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Kopylov A. M. et al. RNA Aptamers for Theranostics of Glioblastoma of Human Brain // Biochemistry (Moscow). 2021. Vol. 86. No. 8. pp. 1012-1024.
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Kopylov A. M., Fab L. V., Antipova O., Savchenko E. A., Revishchin A. V., Parshina V. V., Pavlova S. V., Kireev I. I., Golovin A. V., Usachev D. Y., Pavlova G. V. RNA Aptamers for Theranostics of Glioblastoma of Human Brain // Biochemistry (Moscow). 2021. Vol. 86. No. 8. pp. 1012-1024.
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TY - JOUR
DO - 10.1134/S0006297921080113
UR - https://doi.org/10.1134%2FS0006297921080113
TI - RNA Aptamers for Theranostics of Glioblastoma of Human Brain
T2 - Biochemistry (Moscow)
AU - Fab, Lika V
AU - Revishchin, Alexander V
AU - Kireev, Igor I
AU - Usachev, Dmitry Y
AU - Savchenko, Ekaterina A
AU - Parshina, Viktoriya V
AU - Pavlova, Svetlana V
AU - Pavlova, Galina V
AU - Antipova, Olga
AU - Golovin, Andrey V.
AU - Kopylov, Alexey M
PY - 2021
DA - 2021/08/13 00:00:00
PB - Pleiades Publishing
SP - 1012-1024
IS - 8
VL - 86
SN - 0006-2979
SN - 1608-3040
ER -
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@article{2021_Kopylov,
author = {Lika V Fab and Alexander V Revishchin and Igor I Kireev and Dmitry Y Usachev and Ekaterina A Savchenko and Viktoriya V Parshina and Svetlana V Pavlova and Galina V Pavlova and Olga Antipova and Andrey V. Golovin and Alexey M Kopylov},
title = {RNA Aptamers for Theranostics of Glioblastoma of Human Brain},
journal = {Biochemistry (Moscow)},
year = {2021},
volume = {86},
publisher = {Pleiades Publishing},
month = {aug},
url = {https://doi.org/10.1134%2FS0006297921080113},
number = {8},
pages = {1012--1024},
doi = {10.1134/S0006297921080113}
}
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Kopylov, Alexey M., et al. “RNA Aptamers for Theranostics of Glioblastoma of Human Brain.” Biochemistry (Moscow), vol. 86, no. 8, Aug. 2021, pp. 1012-1024. https://doi.org/10.1134%2FS0006297921080113.
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