Biophysics (Russian Federation)

, том 52 , издание 6 , страницы 575-581

Protein-filled polyelectrolyte microcapsules in the design of enzymic microdiagnostics

B.I. Sukhorukov ¹

S. A. Tikhonenko ¹

E A Saburova ¹

A V Dubrovskii ¹

Yu N Dybovskaya ¹

L.I. Shabarchina ¹

Скрыть аффилиации авторов Показать аффилиации авторов: 1 аффилиация

institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia |

Тип публикации: Journal Article

Дата публикации: 2007-12-01

Pleiades Publishing

Biophysics (Russian Federation)

scimago Q4

БС3

SJR: 0.185

CiteScore: 1.2

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ISSN: 00063509, 15556654

DOI: 10.1134/S0006350907060085

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Biophysics

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Encapsulation of enzymes (lactate dehydrogenase and urease) in polyelectrolyte shells was assessed with a view to designing enzymic microdiagnostics for low-molecular compounds in native biological fluids. Polyelectrolyte microcapsules were prepared with two polyanions [poly(styrenesulfonate) PSS and dextran sulfate DS] and two polycations [poly(allylamine) PAA and poly(diallyldimethylammonium) PDADMA]; calcium carbonate microspherulites with embedded enzymes served as “cores.” It was demonstrated that the main problem in making such a biosensor is to select a pair of oppositely charged polyelectrolytes that would be optimal for enzyme functioning. The best pairs were PAA/DS and PAA/PSS for lactate dehydrogenase, and PSS/PAA and PSS/PDADMA for urease. We designed and prepared enzyme-containing microcapsules differing in polyelectrolyte composition and number of layers, and investigated their properties.

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Ким Александр

34 публикации, 190 цитирований, 5 рецензий

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Институт теоретической и экспериментальной биофизики РАН

7 цитирований

Мусин Егор

PhD, доц.

26 публикаций, 156 цитирований, 62 рецензии

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Институт теоретической и экспериментальной биофизики РАН

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Кузнецова Ирен

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Институт радиотехники и электроники имени В.А. Котельникова РАН

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Sukhorukov B. et al. Protein-filled polyelectrolyte microcapsules in the design of enzymic microdiagnostics // Biophysics (Russian Federation). 2007. Vol. 52. No. 6. pp. 575-581.

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Sukhorukov B., Tikhonenko S. A., Saburova E. A., Dubrovskii A. V., Dybovskaya Yu. N., Shabarchina L. Protein-filled polyelectrolyte microcapsules in the design of enzymic microdiagnostics // Biophysics (Russian Federation). 2007. Vol. 52. No. 6. pp. 575-581.

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TY - JOUR

DO - 10.1134/S0006350907060085

UR - http://link.springer.com/10.1134/S0006350907060085

TI - Protein-filled polyelectrolyte microcapsules in the design of enzymic microdiagnostics

T2 - Biophysics (Russian Federation)

AU - Sukhorukov, B.I.

AU - Tikhonenko, S. A.

AU - Saburova, E A

AU - Dubrovskii, A V

AU - Dybovskaya, Yu N

AU - Shabarchina, L.I.

PY - 2007

DA - 2007/12/01

PB - Pleiades Publishing

SP - 575-581

IS - 6

VL - 52

SN - 0006-3509

SN - 1555-6654

ER -

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@article{2007_Sukhorukov,

author = {B.I. Sukhorukov and S. A. Tikhonenko and E A Saburova and A V Dubrovskii and Yu N Dybovskaya and L.I. Shabarchina},

title = {Protein-filled polyelectrolyte microcapsules in the design of enzymic microdiagnostics},

journal = {Biophysics (Russian Federation)},

year = {2007},

volume = {52},

publisher = {Pleiades Publishing},

month = {dec},

url = {http://link.springer.com/10.1134/S0006350907060085},

number = {6},

pages = {575--581},

doi = {10.1134/S0006350907060085}

}

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Sukhorukov, B.I., et al. “Protein-filled polyelectrolyte microcapsules in the design of enzymic microdiagnostics.” Biophysics (Russian Federation), vol. 52, no. 6, Dec. 2007, pp. 575-581. http://link.springer.com/10.1134/S0006350907060085.