Biophysics (Russian Federation)

, том 57 , издание 3 , страницы 336-342

Obtaining phage mini-antibodies and using them for detection of microbial cells with an electroacoustic sensor

O I Guliy ¹

B D Zaitsev ²

I. E. Kuznetsova ²

A M Shikhabudinov ²

O.A. Karavaeva ¹

L A Dykman ¹

S A Staroverov ¹

O. V. Ignatov ¹

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Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia |

Saratov Branch, Institute of Radio Engineering and Electronics, Russian Academy of Sciences, Saratov, Russia |

Тип публикации: Journal Article

Дата публикации: 2012-05-01

Pleiades Publishing

Biophysics (Russian Federation)

scimago Q4

БС3

SJR: 0.185

CiteScore: 1.2

Impact factor: —

ISSN: 00063509, 15556654

DOI: 10.1134/S0006350912030086

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Biophysics

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Phage mini-antibodies to bacterial cells of strain Azospirillum brasilense Sp245 were obtained, and the possibility of using them for detection of microbial cells with a lateral field excited piezoelectric resonator was studied. It has been found that the frequency dependences of the real and imaginary parts of electrical impedance of such a resonator loaded with a suspension of A. brasilense Sp245 cells with the mini-antibodies differ significantly from the dependences of the resonator with a control cell suspension without mini-anti-bodies. The limit of possible determination of the concentration of microbial cells is found to be 103 cells/mL upon interaction with mini-antibodies. It has been ascertained that detection of A. brasilense Sp245 cells with the aid of mini-antibodies is possible even in the presence of other cultures, for example, E. coli BL-Ril and A. brasilense Sp7. Therefore, it has been shown for the first time that detection of microbial cells with an electroacoustic sensor is feasible.

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Guliy O. I. et al. Obtaining phage mini-antibodies and using them for detection of microbial cells with an electroacoustic sensor // Biophysics (Russian Federation). 2012. Vol. 57. No. 3. pp. 336-342.

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Guliy O. I., Zaitsev B. D., Kuznetsova I. E., Shikhabudinov A. M., Karavaeva O., Dykman L. A., Staroverov S. A., Ignatov O. V. Obtaining phage mini-antibodies and using them for detection of microbial cells with an electroacoustic sensor // Biophysics (Russian Federation). 2012. Vol. 57. No. 3. pp. 336-342.

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TY - JOUR

DO - 10.1134/S0006350912030086

UR - http://link.springer.com/10.1134/S0006350912030086

TI - Obtaining phage mini-antibodies and using them for detection of microbial cells with an electroacoustic sensor

T2 - Biophysics (Russian Federation)

AU - Guliy, O I

AU - Zaitsev, B D

AU - Kuznetsova, I. E.

AU - Shikhabudinov, A M

AU - Karavaeva, O.A.

AU - Dykman, L A

AU - Staroverov, S A

AU - Ignatov, O. V.

PY - 2012

DA - 2012/05/01

PB - Pleiades Publishing

SP - 336-342

IS - 3

VL - 57

SN - 0006-3509

SN - 1555-6654

ER -

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@article{2012_Guliy,

author = {O I Guliy and B D Zaitsev and I. E. Kuznetsova and A M Shikhabudinov and O.A. Karavaeva and L A Dykman and S A Staroverov and O. V. Ignatov},

title = {Obtaining phage mini-antibodies and using them for detection of microbial cells with an electroacoustic sensor},

journal = {Biophysics (Russian Federation)},

year = {2012},

volume = {57},

publisher = {Pleiades Publishing},

month = {may},

url = {http://link.springer.com/10.1134/S0006350912030086},

number = {3},

pages = {336--342},

doi = {10.1134/S0006350912030086}

}

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Guliy, O. I., et al. “Obtaining phage mini-antibodies and using them for detection of microbial cells with an electroacoustic sensor.” Biophysics (Russian Federation), vol. 57, no. 3, May. 2012, pp. 336-342. http://link.springer.com/10.1134/S0006350912030086.