Obtaining phage mini-antibodies and using them for detection of microbial cells with an electroacoustic sensor
O I Guliy
1
,
B D Zaitsev
2
,
I. E. Kuznetsova
2
,
A M Shikhabudinov
2
,
O.A. Karavaeva
1
,
L A Dykman
1
,
S A Staroverov
1
,
O. V. Ignatov
1
Publication type: Journal Article
Publication date: 2012-05-01
scimago Q4
SJR: 0.185
CiteScore: 1.2
Impact factor: —
ISSN: 00063509, 15556654
Biophysics
Abstract
Phage mini-antibodies to bacterial cells of strain Azospirillum brasilense Sp245 were obtained, and the possibility of using them for detection of microbial cells with a lateral field excited piezoelectric resonator was studied. It has been found that the frequency dependences of the real and imaginary parts of electrical impedance of such a resonator loaded with a suspension of A. brasilense Sp245 cells with the mini-antibodies differ significantly from the dependences of the resonator with a control cell suspension without mini-anti-bodies. The limit of possible determination of the concentration of microbial cells is found to be 103 cells/mL upon interaction with mini-antibodies. It has been ascertained that detection of A. brasilense Sp245 cells with the aid of mini-antibodies is possible even in the presence of other cultures, for example, E. coli BL-Ril and A. brasilense Sp7. Therefore, it has been shown for the first time that detection of microbial cells with an electroacoustic sensor is feasible.
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Guliy O. I. et al. Obtaining phage mini-antibodies and using them for detection of microbial cells with an electroacoustic sensor // Biophysics (Russian Federation). 2012. Vol. 57. No. 3. pp. 336-342.
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Guliy O. I., Zaitsev B. D., Kuznetsova I. E., Shikhabudinov A. M., Karavaeva O., Dykman L. A., Staroverov S. A., Ignatov O. V. Obtaining phage mini-antibodies and using them for detection of microbial cells with an electroacoustic sensor // Biophysics (Russian Federation). 2012. Vol. 57. No. 3. pp. 336-342.
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RIS
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TY - JOUR
DO - 10.1134/S0006350912030086
UR - http://link.springer.com/10.1134/S0006350912030086
TI - Obtaining phage mini-antibodies and using them for detection of microbial cells with an electroacoustic sensor
T2 - Biophysics (Russian Federation)
AU - Guliy, O I
AU - Zaitsev, B D
AU - Kuznetsova, I. E.
AU - Shikhabudinov, A M
AU - Karavaeva, O.A.
AU - Dykman, L A
AU - Staroverov, S A
AU - Ignatov, O. V.
PY - 2012
DA - 2012/05/01
PB - Pleiades Publishing
SP - 336-342
IS - 3
VL - 57
SN - 0006-3509
SN - 1555-6654
ER -
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BibTex (up to 50 authors)
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@article{2012_Guliy,
author = {O I Guliy and B D Zaitsev and I. E. Kuznetsova and A M Shikhabudinov and O.A. Karavaeva and L A Dykman and S A Staroverov and O. V. Ignatov},
title = {Obtaining phage mini-antibodies and using them for detection of microbial cells with an electroacoustic sensor},
journal = {Biophysics (Russian Federation)},
year = {2012},
volume = {57},
publisher = {Pleiades Publishing},
month = {may},
url = {http://link.springer.com/10.1134/S0006350912030086},
number = {3},
pages = {336--342},
doi = {10.1134/S0006350912030086}
}
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MLA
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Guliy, O. I., et al. “Obtaining phage mini-antibodies and using them for detection of microbial cells with an electroacoustic sensor.” Biophysics (Russian Federation), vol. 57, no. 3, May. 2012, pp. 336-342. http://link.springer.com/10.1134/S0006350912030086.
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