Cell Wall Galactofuranan of “Paenarthrobacter pyridinovorans” VKM Ac-1098D

The structure of the cell wall glycopolymer and the taxonomic position of the pyridine-degrading strain VKM Ac-1098D were established. By using chemical and NMR spectroscopic methods, the glycopopolymer was identified as 1,6-linked β-D-galactofuranan with side diaminoglucose residue (2,3-diacetamido-2,3-dideoxy-β-glucopyranose, β-GlcpNAc3NAc). This polymer structure has not been previously described in prokaryotes. Structurally similar galactofuranan which differed from the polymer of strain VKM Ac-1098D by the α-configuration of diaminoglucose (α-GlcpNAc3NAc) in the side chain was found previously in some studied Paenarthrobacter species. The results of the 16S rRNA gene sequence analysis and MALDI-TOF clustering showed that strain VKM Ac-1098D represents a new species of the genus Paenarthrobacter tentatively named “Paenarthrobacter pyridinovorans”. The data obtained in this and earlier works indicate that galactofuranan with diaminoglucose in the side chain may serve as a chemotaxonomic marker of the genus Paenarthrobacter and the α-configuration of the glycosidic bond of diaminoglucose may be a feature characteristic of the putative new species.