Head of Laboratory

Evtushenko, Lyudmila

DSc in Biological/biomedical sciences, Professor
Publications
110
Citations
1 619
h-index
21
Authorization required.

- The study of the biological (genetic) diversity of microorganisms living in various ecosystems and associated with higher organisms;

- Introduction to culture and comprehensive study of new microorganisms of all major super-kingdoms and various physiological groups, identification and description of new taxa, improvement of the classification system and identification methods, including at the level of species (subspecies);

- Search and study of new biopolymers, enzymes and secondary metabolites of microbial origin;

- Study of the possibilities of using anaerobic bacteria and archaea to produce biohydrogen and biogas, antifreeze proteins and cold-resistant enzymes;

- Sequencing and annotation of complete genomes of bacteria and fungi to expand the information base for the development of the concept of species in microorganisms and the construction of a modern "single tree of life", understanding strategies for adapting microorganisms to extreme conditions, mechanisms of symbiotic interactions and phytopathogenicity, the search for new enzymes and their complexes of fundamental and applied importance;

- Development of accelerated methods for identification of microorganisms at the species (subspecies) level based on MALDI mass spectrometry and analysis of "housing-keeping" genes.

  1. MALDI mass spectroscopy
  2. Chromatography
  3. Working with bacterial strains
  4. Gas chromatography
  5. High-efficiency liquid chromatography (HPLC)
  6. Spectrophotometry
  7. Lyophilization of microbial cultures
  8. Genome annotation
  9. Working with yeast strains
  10. Working with strains of mycelial fungi
  11. Working with archaea strains
Lyudmila Evtushenko
Head of Laboratory
Yury Ospennikov
Junior researcher

Research directions

Abstract of complete genomes of microorganisms

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Abstract of complete genomes of microorganisms (including for the description of new taxa, understanding the mechanisms of symbiotic interactions and phytopathogenicity, searching for new enzymes and their complexes of fundamental and applied importance).

Analysis (determination) of products of metabolism of microorganisms

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Analysis of microbial metabolic products using HPLC and gas chromatography. Data on metabolic products (organic acids, mono- and polyatomic alcohols, esters and gaseous products) are among the important taxonomic characteristics of a number of groups of bacteria and archaea and are necessary for the description of new species and genera. The determination of the above properties also makes it possible to evaluate the possibility of using the studied microorganisms as producers of the corresponding compounds.

Determination of chemotaxonomic characteristics of microbial strains

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Determination of taxonomic characteristics of strains of microorganisms (bacteria, archaea, fungi, yeast) required to describe new species and genera of microorganisms in accordance with international standards. The list of such characteristics includes: the isomer of diaminopimelic acid of the cell wall, the composition of amino acids of the cell wall, the composition of sugars of whole cells/cell walls, the type of mycolic acids, the composition of fatty acids, the composition of isoprenoid quinones of the respiratory chain (menaquinones, ubiquinones), the composition of phospholipids, the nucleotide composition of DNA.

Identification of microbial strains

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Identification of microbial strains (the MALDI method of mass spectrometry) using the manufacturer's database (Brooker) and local databases created at the CCP VKM. The method is based on comparing the mass spectra of a mixture of components of destroyed cells (the components of the MALDI spectra in the mass range 2000-20000 m/z correspond to polar cytoplasmic proteins, mainly ribosomal). The advantages of the method are the speed and small amount of material for analysis (one colony is enough).

Phylogenetic analysis of microorganisms

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Phylogenetic analysis of microorganisms based on 16S rRNA gene sequences and "housing-keeping" genes (gyrB, RecA, rpoB, etc.) in order to determine the generic and species belonging of microorganisms

Publications and patents

Анатолий Григорьевич Козловский (RU), Анатолий Григорьевич Козловский, Валентина Павловна Желифонова (RU), Валентина Павловна Желифонова, Татьяна Валентиновна Антипова (RU), Татьяна Валентиновна Антипова, Светлана Михайловна Озерская (RU), Светлана Михайловна Озерская, Галина Александровна Кочкина (RU), Галина Александровна Кочкина, Наталия Евгеньевна Иванушкина (RU), Наталия Евгеньевна Иванушкина
RU2386692C1, 2010

Partners

Lab address

Пущино, проспект Науки, д. 5
Authorization required.