Molecular Biology, volume 41, issue 6, pages 939-948

The interaction with Escherichia coli 23S rRNA helices 89 and 91 contributes to the IF2 activity but is insignificant for the functioning of the elongation factors

Publication typeJournal Article
Publication date2007-12-19
Quartile SCImago
Q4
Quartile WOS
Q4
Impact factor1.2
ISSN00268933, 16083245
Structural Biology
Biophysics
Abstract
The noncanonical pairing of C2475 with G2529 links 23S rRNA helices 89 and 91 in the Escherichia coli ribosome. These nucleotides are at the intersection of the peptidyltransferase center, the sarcin-ricin loop, and the GTPase-associated center of the ribosome. The functional significance of C2475 and G2529 was studied using the C2475G, C2475G/G2529C, and ΔA2471/U2479 mutations of the 23S rRNA. The mutations did not change the activity of the elongation factors, but affected the cell growth rate, the 23S rRNA conformation, and the translation initiation. The C2475G and C2475G/G2529C mutations substantially affected the binding of the IF2 · GDPNP complex to the ribosome and the IF2-dependent formation of the initiation complex and increased the ribosome-stimulated GTPase activity of IF2. The Δ A2471/U2479 mutation did not affect the binding of IF2 · GDPNP to the ribosome, but influenced the IF2-dependent formation of the initiation complex and GTPase activity of IF2. The contact between helices 89 and 91 was found to be important for the efficient translation initiation catalyzed by IF2.
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Burakovsky D. E. et al. The interaction with Escherichia coli 23S rRNA helices 89 and 91 contributes to the IF2 activity but is insignificant for the functioning of the elongation factors // Molecular Biology. 2007. Vol. 41. No. 6. pp. 939-948.
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Burakovsky D. E., Smirnova A. S., Lesnyak D. V., Kiparisov S. V., Leonov A. A., Sergiev P. V., Bogdanov A. A., Dontsova O. A. The interaction with Escherichia coli 23S rRNA helices 89 and 91 contributes to the IF2 activity but is insignificant for the functioning of the elongation factors // Molecular Biology. 2007. Vol. 41. No. 6. pp. 939-948.
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TY - JOUR
DO - 10.1134/S0026893307060118
UR - https://doi.org/10.1134%2FS0026893307060118
TI - The interaction with Escherichia coli 23S rRNA helices 89 and 91 contributes to the IF2 activity but is insignificant for the functioning of the elongation factors
T2 - Molecular Biology
AU - Burakovsky, D E
AU - Smirnova, A. S.
AU - Lesnyak, D V
AU - Kiparisov, S V
AU - Leonov, A. A.
AU - Sergiev, P V
AU - Bogdanov, A. A.
AU - Dontsova, O. A.
PY - 2007
DA - 2007/12/19 00:00:00
PB - Pleiades Publishing
SP - 939-948
IS - 6
VL - 41
SN - 0026-8933
SN - 1608-3245
ER -
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@article{2007_Burakovsky
author = {D E Burakovsky and A. S. Smirnova and D V Lesnyak and S V Kiparisov and A. A. Leonov and P V Sergiev and A. A. Bogdanov and O. A. Dontsova},
title = {The interaction with Escherichia coli 23S rRNA helices 89 and 91 contributes to the IF2 activity but is insignificant for the functioning of the elongation factors},
journal = {Molecular Biology},
year = {2007},
volume = {41},
publisher = {Pleiades Publishing},
month = {dec},
url = {https://doi.org/10.1134%2FS0026893307060118},
number = {6},
pages = {939--948},
doi = {10.1134/S0026893307060118}
}
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Burakovsky, D. E., et al. “The interaction with Escherichia coli 23S rRNA helices 89 and 91 contributes to the IF2 activity but is insignificant for the functioning of the elongation factors.” Molecular Biology, vol. 41, no. 6, Dec. 2007, pp. 939-948. https://doi.org/10.1134%2FS0026893307060118.
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