Molecular Biology, том 41, издание 6, номера страниц: 939-948

The interaction with Escherichia coli 23S rRNA helices 89 and 91 contributes to the IF2 activity but is insignificant for the functioning of the elongation factors

Burakovsky D. E. 1
Lesnyak D. V. 2
Kiparisov S. V. 1
Leonov A. A. 1
Bogdanov A. A. 1
Тип документаJournal Article
Дата публикации2007-12
ИздательPleiades Publishing
Название журналаMolecular Biology
Квартиль по SCImagoQ4
Квартиль по Web of ScienceQ4
Импакт-фактор 20211.54
ISSN00268933, 16083245
Structural Biology
Biophysics
Краткое описание
The noncanonical pairing of C2475 with G2529 links 23S rRNA helices 89 and 91 in the Escherichia coli ribosome. These nucleotides are at the intersection of the peptidyltransferase center, the sarcin-ricin loop, and the GTPase-associated center of the ribosome. The functional significance of C2475 and G2529 was studied using the C2475G, C2475G/G2529C, and ΔA2471/U2479 mutations of the 23S rRNA. The mutations did not change the activity of the elongation factors, but affected the cell growth rate, the 23S rRNA conformation, and the translation initiation. The C2475G and C2475G/G2529C mutations substantially affected the binding of the IF2 · GDPNP complex to the ribosome and the IF2-dependent formation of the initiation complex and increased the ribosome-stimulated GTPase activity of IF2. The Δ A2471/U2479 mutation did not affect the binding of IF2 · GDPNP to the ribosome, but influenced the IF2-dependent formation of the initiation complex and GTPase activity of IF2. The contact between helices 89 and 91 was found to be important for the efficient translation initiation catalyzed by IF2.
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1. Burakovsky D.E. и др. The interaction with Escherichia coli 23S rRNA helices 89 and 91 contributes to the IF2 activity but is insignificant for the functioning of the elongation factors // Molecular Biology. 2007. Т. 41. № 6. С. 939–948.
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TY - JOUR

DO - 10.1134/s0026893307060118

UR - http://dx.doi.org/10.1134/S0026893307060118

TI - The interaction with Escherichia coli 23S rRNA helices 89 and 91 contributes to the IF2 activity but is insignificant for the functioning of the elongation factors

T2 - Molecular Biology

AU - Burakovsky, D. E.

AU - Smirnova, A. S.

AU - Lesnyak, D. V.

AU - Kiparisov, S. V.

AU - Leonov, A. A.

AU - Sergiev, P. V.

AU - Bogdanov, A. A.

AU - Dontsova, O. A.

PY - 2007

DA - 2007/12

PB - Pleiades Publishing Ltd

SP - 939-948

IS - 6

VL - 41

SN - 0026-8933

SN - 1608-3245

ER -

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@article{2007,

doi = {10.1134/s0026893307060118},

url = {https://doi.org/10.1134%2Fs0026893307060118},

year = 2007,

month = {dec},

publisher = {Pleiades Publishing Ltd},

volume = {41},

number = {6},

pages = {939--948},

author = {D. E. Burakovsky and A. S. Smirnova and D. V. Lesnyak and S. V. Kiparisov and A. A. Leonov and P. V. Sergiev and A. A. Bogdanov and O. A. Dontsova},

title = {The interaction with Escherichia coli 23S {rRNA} helices 89 and 91 contributes to the {IF}2 activity but is insignificant for the functioning of the elongation factors}

}

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Burakovsky, D. E. et al. “The Interaction with Escherichia Coli 23S rRNA Helices 89 and 91 Contributes to the IF2 Activity but Is Insignificant for the Functioning of the Elongation Factors.” Molecular Biology 41.6 (2007): 939–948. Crossref. Web.