Pleiades Publishing
Molecular Biology
,  том 51 ,  издание 1 ,  страницы 72-79

RNA immunoprecipitation technique for Drosophila melanogaster S2 cells

Тип публикации: Journal Article
Дата публикации: 2017-01-01
Pleiades Publishing
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БС3
SJR: 0.331
CiteScore: 1.6
Impact factor: 1.2
ISSN: 00268933, 16083245, 21689547
Structural Biology
Biophysics
Краткое описание
RNA-binding proteins play an important role in RNA metabolism, especially in mRNA biogenesis and subsequent expression patterns regulation. RNA immunoprecipitation (RIP) is a powerful tool for detecting protein–RNA associations. In this paper, we briefly cover the history of this method for analyzing RNA–protein interactions and reviewing a number of modifications of the RIP technique. We also present an adjusted RIP protocol that was modified for Drosophila S2 cell culture. The use of this protocol allows one to perform the efficient precipitation of RNA–protein complexes and harvest RNA in amounts that are sufficient for its downstream analysis.
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Kachaev Z. M. et al. RNA immunoprecipitation technique for Drosophila melanogaster S2 cells // Molecular Biology. 2017. Vol. 51. No. 1. pp. 72-79.
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Kachaev Z. M., Gilmutdinov R. A., Kopytova D. V., Zheludkevich A. A., Shidlovskii Y. V., Kurbidaeva A. S. RNA immunoprecipitation technique for Drosophila melanogaster S2 cells // Molecular Biology. 2017. Vol. 51. No. 1. pp. 72-79.
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TY - JOUR
DO - 10.1134/S002689331606008X
UR - https://doi.org/10.1134/S002689331606008X
TI - RNA immunoprecipitation technique for Drosophila melanogaster S2 cells
T2 - Molecular Biology
AU - Kachaev, Z M
AU - Gilmutdinov, R A
AU - Kopytova, D V
AU - Zheludkevich, A A
AU - Shidlovskii, Y V
AU - Kurbidaeva, A. S.
PY - 2017
DA - 2017/01/01
PB - Pleiades Publishing
SP - 72-79
IS - 1
VL - 51
SN - 0026-8933
SN - 1608-3245
SN - 2168-9547
ER -
BibTex |
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@article{2017_Kachaev,
author = {Z M Kachaev and R A Gilmutdinov and D V Kopytova and A A Zheludkevich and Y V Shidlovskii and A. S. Kurbidaeva},
title = {RNA immunoprecipitation technique for Drosophila melanogaster S2 cells},
journal = {Molecular Biology},
year = {2017},
volume = {51},
publisher = {Pleiades Publishing},
month = {jan},
url = {https://doi.org/10.1134/S002689331606008X},
number = {1},
pages = {72--79},
doi = {10.1134/S002689331606008X}
}
MLA
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Kachaev, Z. M., et al. “RNA immunoprecipitation technique for Drosophila melanogaster S2 cells.” Molecular Biology, vol. 51, no. 1, Jan. 2017, pp. 72-79. https://doi.org/10.1134/S002689331606008X.