Preparation and analysis of nuclear protein extract from Drosophila melanogaster embryos for studying transcription factors
Publication type: Journal Article
Publication date: 2016-11-01
scimago Q4
wos Q3
SJR: 0.267
CiteScore: 1.9
Impact factor: 1.7
ISSN: 10681620, 1608330X, 15739163
Organic Chemistry
Biochemistry
Abstract
Nuclear protein extract is an important substance for studying different molecular systems of the cell, particularly transcription machinery. The paper briefly describes extraction methods and provides a protocol for the preparation of extract from nuclei of Drosophila embryos using salt extraction. The extraction efficiency of transcription factors and chromatin proteins has been checked under different salt concentrations. The paper also provides data about chromatographic properties of some protein factors regulating gene activity.
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GOST
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Shaposhnikov A. V. et al. Preparation and analysis of nuclear protein extract from Drosophila melanogaster embryos for studying transcription factors // Russian Journal of Bioorganic Chemistry. 2016. Vol. 42. No. 6. pp. 646-654.
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Shaposhnikov A. V., Lebedeva L. A., Chernioglo E. S., Kachaev Z. M., Abdrakhmanov A., Shidlovskii Yu. V. Preparation and analysis of nuclear protein extract from Drosophila melanogaster embryos for studying transcription factors // Russian Journal of Bioorganic Chemistry. 2016. Vol. 42. No. 6. pp. 646-654.
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RIS
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TY - JOUR
DO - 10.1134/S1068162016060108
UR - https://doi.org/10.1134/S1068162016060108
TI - Preparation and analysis of nuclear protein extract from Drosophila melanogaster embryos for studying transcription factors
T2 - Russian Journal of Bioorganic Chemistry
AU - Shaposhnikov, A. V.
AU - Lebedeva, L. A.
AU - Chernioglo, E S
AU - Kachaev, Z M
AU - Abdrakhmanov, A.
AU - Shidlovskii, Yu V
PY - 2016
DA - 2016/11/01
PB - Pleiades Publishing
SP - 646-654
IS - 6
VL - 42
SN - 1068-1620
SN - 1608-330X
SN - 1573-9163
ER -
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BibTex (up to 50 authors)
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@article{2016_Shaposhnikov,
author = {A. V. Shaposhnikov and L. A. Lebedeva and E S Chernioglo and Z M Kachaev and A. Abdrakhmanov and Yu V Shidlovskii},
title = {Preparation and analysis of nuclear protein extract from Drosophila melanogaster embryos for studying transcription factors},
journal = {Russian Journal of Bioorganic Chemistry},
year = {2016},
volume = {42},
publisher = {Pleiades Publishing},
month = {nov},
url = {https://doi.org/10.1134/S1068162016060108},
number = {6},
pages = {646--654},
doi = {10.1134/S1068162016060108}
}
Cite this
MLA
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Shaposhnikov, A. V., et al. “Preparation and analysis of nuclear protein extract from Drosophila melanogaster embryos for studying transcription factors.” Russian Journal of Bioorganic Chemistry, vol. 42, no. 6, Nov. 2016, pp. 646-654. https://doi.org/10.1134/S1068162016060108.
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