Intermediates of the Autocatalytic Reaction of the Formation of a Chromophore in a Green Fluorescent Protein

The autocatalytic formation of a chromophore in a green fluorescent protein, which is actively used as a marker for visualizing processes in living systems, is an important example of multistage reactions in protein macromolecules. The kinetic, spectral, and X-ray structural experimental studies and supercomputer calculations by molecular modeling methods are devoted to the study of intermediates of the elementary reaction stages, which are grouped into cyclization and oxidation blocks. The article analyzes the results, compatible with the experimental data, of the structure of intermediates of the reactions of chromophore formation for the native protein form obtained through computer modeling, with the aim of including this information in the database of the reactions’ intermediates in enzymatic reactions.