Isolation and characterization of extracellular pectin lyase from Penicillium canescens
O A Sinitsyna
1
,
E. A. Fedorova
1
,
M V Semenova
1
,
A.V. Gusakov
1
,
L M Sokolova
2
,
T M Bubnova
2
,
O. N. Okunev
2
,
A. M. Chulkin
3
,
E. A. Vavilova
3
,
Y P Vinetsky
3
,
A. P. Sinitsyn
1, 4
Publication type: Journal Article
Publication date: 2007-05-25
scimago Q2
wos Q4
SJR: 0.659
CiteScore: 3.8
Impact factor: 2.2
ISSN: 00062979, 16083040
PubMed ID:
17573712
Biochemistry
General Medicine
Abstract
Pectin lyase A (molecular weight 38 kD by SDS-PAGE, pI 6.7) was purified to homogeneity from culture broth of the myoelial fungus Penicillium canescens using chromatographic techniques. During genomic library screening, the gene encoding pectin lyase A from P. canescens (pelA) was isolated and sequenced, and the amino acid sequence was generated by applying the multiple alignment procedure (360 residues). A theoretical model for the three dimensional structure of the protein molecule was also proposed. Different properties of pectin lyase A were investigated: substrate specificity, pH-and temperature optimum of activity, stability under different pH and temperature conditions, and the effect of Ca2+ on enzyme activity. In the course of the laboratory trials, it was demonstrated that pectin lyase A from P. canescens could be successfully applied to production and clarification of juice.
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Total citations:
30
Citations from 2025:
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(3.33%)
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GOST
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Sinitsyna O. A. et al. Isolation and characterization of extracellular pectin lyase from Penicillium canescens // Biochemistry (Moscow). 2007. Vol. 72. No. 5. pp. 565-571.
GOST all authors (up to 50)
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Sinitsyna O. A., Fedorova E. A., Semenova M. V., Gusakov A., Sokolova L. M., Bubnova T. M., Okunev O. N., Chulkin A. M., Vavilova E. A., Vinetsky Y. P., Sinitsyn A. P. Isolation and characterization of extracellular pectin lyase from Penicillium canescens // Biochemistry (Moscow). 2007. Vol. 72. No. 5. pp. 565-571.
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RIS
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TY - JOUR
DO - 10.1134/s0006297907050148
UR - https://doi.org/10.1134/s0006297907050148
TI - Isolation and characterization of extracellular pectin lyase from Penicillium canescens
T2 - Biochemistry (Moscow)
AU - Sinitsyna, O A
AU - Fedorova, E. A.
AU - Semenova, M V
AU - Gusakov, A.V.
AU - Sokolova, L M
AU - Bubnova, T M
AU - Okunev, O. N.
AU - Chulkin, A. M.
AU - Vavilova, E. A.
AU - Vinetsky, Y P
AU - Sinitsyn, A. P.
PY - 2007
DA - 2007/05/25
PB - Pleiades Publishing
SP - 565-571
IS - 5
VL - 72
PMID - 17573712
SN - 0006-2979
SN - 1608-3040
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2007_Sinitsyna,
author = {O A Sinitsyna and E. A. Fedorova and M V Semenova and A.V. Gusakov and L M Sokolova and T M Bubnova and O. N. Okunev and A. M. Chulkin and E. A. Vavilova and Y P Vinetsky and A. P. Sinitsyn},
title = {Isolation and characterization of extracellular pectin lyase from Penicillium canescens},
journal = {Biochemistry (Moscow)},
year = {2007},
volume = {72},
publisher = {Pleiades Publishing},
month = {may},
url = {https://doi.org/10.1134/s0006297907050148},
number = {5},
pages = {565--571},
doi = {10.1134/s0006297907050148}
}
Cite this
MLA
Copy
Sinitsyna, O. A., et al. “Isolation and characterization of extracellular pectin lyase from Penicillium canescens.” Biochemistry (Moscow), vol. 72, no. 5, May. 2007, pp. 565-571. https://doi.org/10.1134/s0006297907050148.