Open Access
Journal of Nanobiotechnology, volume 17, issue 1, publication number 27
Long-term live cells observation of internalized fluorescent Fe@C nanoparticles in constant magnetic field
Garanina Anastasiia
1, 2, 3
,
Kireev Igor
4
,
Zhironkina Oxana
4
,
Strelkova Olga
4
,
Shakhov Anton
4
,
Alieva Irina
4
,
Davydov Valery
5
,
Murugesan Sankaran
6
,
Khabashesku Valery
6
,
Majouga Alexander
2, 3, 7
,
Agafonov Viatcheslav
1
,
Uzbekov Rustem
8, 9
1
GREMAN, UMR CNRS 7347, Université de Tours, Tours, France
|
6
Center for Technology Innovation, Baker Hughes a GE Company, Houston, USA
|
8
Faculté de médecine, Université François Rabelais, Tours, France
|
Publication type: Journal Article
Publication date: 2019-02-06
Journal:
Journal of Nanobiotechnology
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 10.2
ISSN: 14773155
PubMed ID:
30728022
Medicine (miscellaneous)
Pharmaceutical Science
Molecular Medicine
Applied Microbiology and Biotechnology
Bioengineering
Biomedical Engineering
Abstract
BackgroundTheranostics application of superparamagnetic nanoparticles based on magnetite and maghemite is impeded by their toxicity. The use of additional protective shells significantly reduced the magnetic properties of the nanoparticles. Therefore, iron carbides and pure iron nanoparticles coated with multiple layers of onion-like carbon sheath seem to be optimal for biomedicine. Fluorescent markers associated with magnetic nanoparticles provide reliable means for their multimodal visualization. Here, biocompatibility of iron nanoparticles coated with graphite-like shell and labeled with Alexa 647 fluorescent marker has been investigated.MethodsIron core nanoparticles with intact carbon shells were purified by magnetoseparation after hydrochloric acid treatment. The structure of the NPs (nanoparticles) was examined with a high resolution electron microscopy. The surface of the NPs was alkylcarboxylated and further aminated for covalent linking with Alexa Fluor 647 fluorochrome to produce modified fluorescent magnetic nanoparticles (MFMNPs). Live fluorescent imaging and correlative light-electron microscopy were used to study the NPs intracellular distribution and the effects of constant magnetic field on internalized NPs in the cell culture were analyzed. Cell viability was assayed by measuring a proliferative pool with Click-IT labeling.ResultsThe microstructure and magnetic properties of superparamagnetic Fe@C core–shell NPs as well as their endocytosis by living tumor cells, and behavior inside the cells in constant magnetic field (150 mT) were studied. Correlative light-electron microscopy demonstrated that NPs retained their microstructure after internalization by the living cells. Application of constant magnetic field caused orientation of internalized NPs along power lines thus demonstrating their magnetocontrollability. Carbon onion-like shells make these NPs biocompatible and enable long-term observation with confocal microscope. It was found that iron core of NPs shows no toxic effect on the cell physiology, does not inhibit the cell proliferation and also does not induce apoptosis.ConclusionsNon-toxic, biologically compatible superparamagnetic fluorescent MFMNPs can be further used for biological application such as delivery of biologically active compounds both inside the cell and inside the whole organism, magnetic separation, and magnetic resonance imaging (MRI) diagnostics.
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1
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Garanina A. et al. Long-term live cells observation of internalized fluorescent Fe@C nanoparticles in constant magnetic field // Journal of Nanobiotechnology. 2019. Vol. 17. No. 1. 27
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Garanina A., Kireev I., Zhironkina O., Strelkova O., Shakhov A., Alieva I., Davydov V., Murugesan S., Khabashesku V., Majouga A., Agafonov V., Uzbekov R. Long-term live cells observation of internalized fluorescent Fe@C nanoparticles in constant magnetic field // Journal of Nanobiotechnology. 2019. Vol. 17. No. 1. 27
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TY - JOUR
DO - 10.1186/s12951-019-0463-5
UR - https://doi.org/10.1186%2Fs12951-019-0463-5
TI - Long-term live cells observation of internalized fluorescent Fe@C nanoparticles in constant magnetic field
T2 - Journal of Nanobiotechnology
AU - Alieva, Irina
AU - Murugesan, Sankaran
AU - Khabashesku, Valery
AU - Agafonov, Viatcheslav
AU - Uzbekov, Rustem
AU - Kireev, Igor
AU - Majouga, Alexander
AU - Garanina, Anastasiia
AU - Shakhov, Anton
AU - Zhironkina, Oxana
AU - Strelkova, Olga
AU - Davydov, Valery
PY - 2019
DA - 2019/02/06 00:00:00
PB - Springer Nature
IS - 1
VL - 17
PMID - 30728022
SN - 1477-3155
ER -
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@article{2019_Garanina,
author = {Irina Alieva and Sankaran Murugesan and Valery Khabashesku and Viatcheslav Agafonov and Rustem Uzbekov and Igor Kireev and Alexander Majouga and Anastasiia Garanina and Anton Shakhov and Oxana Zhironkina and Olga Strelkova and Valery Davydov},
title = {Long-term live cells observation of internalized fluorescent Fe@C nanoparticles in constant magnetic field},
journal = {Journal of Nanobiotechnology},
year = {2019},
volume = {17},
publisher = {Springer Nature},
month = {feb},
url = {https://doi.org/10.1186%2Fs12951-019-0463-5},
number = {1},
doi = {10.1186/s12951-019-0463-5}
}