Development (Cambridge), volume 148, issue 17
The 3′UTR of the Drosophila CPEB translation factor gene orb2 plays a crucial role in spermatogenesis
Kozlov Eugene
1
,
Yakovlev Konstantin V.
1, 2
,
Kotov Alexei A.
3
,
Barr Justinn
4
,
Zhukova Mariya
1
,
Schedl Paul D
4
,
Shidlovskii Y. V.
1, 5
3
4
Department of Molecular Biology, Princeton University, Princeton, NJ 08544-1014, USA
|
Publication type: Journal Article
Publication date: 2021-09-01
Journal:
Development (Cambridge)
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 4.6
ISSN: 09501991, 14779129
PubMed ID:
34473243
Molecular Biology
Developmental Biology
Abstract
ABSTRACT
CPEB proteins are conserved translation regulators involved in multiple biological processes. One of these proteins in Drosophila, Orb2, is a principal player in spermatogenesis. It is required for meiosis and spermatid differentiation. During the later process, orb2 mRNA and protein are localized within the developing spermatid. To evaluate the role of the orb2 mRNA 3′UTR in spermatogenesis, we used the CRISPR/Cas9 system to generate a deletion of the orb2 3′UTR, orb2R. This deletion disrupts the process of spermatid differentiation but has no apparent effect on meiosis. Differentiation abnormalities include defects in the initial polarization of the 64-cell spermatid cysts, mislocalization of mRNAs and proteins in the elongating spermatid tails, altered morphology of the elongating spermatid tails, and defects in the assembly of the individualization complex. These disruptions in differentiation appear to arise because orb2 mRNA and protein are not properly localized within the 64-cell spermatid cyst.
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Gilmutdinov R. et al. The 3′UTR of the Drosophila CPEB translation factor gene orb2 plays a crucial role in spermatogenesis // Development (Cambridge). 2021. Vol. 148. No. 17.
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Gilmutdinov R., Kozlov E., Yakovlev K. V., Olenina L. V., Kotov A. A., Barr J., Zhukova M., Schedl P. D., Shidlovskii Y. V. The 3′UTR of the Drosophila CPEB translation factor gene orb2 plays a crucial role in spermatogenesis // Development (Cambridge). 2021. Vol. 148. No. 17.
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TY - JOUR
DO - 10.1242/dev.198788
UR - https://doi.org/10.1242%2Fdev.198788
TI - The 3′UTR of the Drosophila CPEB translation factor gene orb2 plays a crucial role in spermatogenesis
T2 - Development (Cambridge)
AU - Gilmutdinov, Rudolf
AU - Kozlov, Eugene
AU - Yakovlev, Konstantin V.
AU - Olenina, Ludmila V.
AU - Kotov, Alexei A.
AU - Barr, Justinn
AU - Zhukova, Mariya
AU - Schedl, Paul D
AU - Shidlovskii, Y. V.
PY - 2021
DA - 2021/09/01 00:00:00
PB - The Company of Biologists
IS - 17
VL - 148
PMID - 34473243
SN - 0950-1991
SN - 1477-9129
ER -
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@article{2021_Gilmutdinov,
author = {Rudolf Gilmutdinov and Eugene Kozlov and Konstantin V. Yakovlev and Ludmila V. Olenina and Alexei A. Kotov and Justinn Barr and Mariya Zhukova and Paul D Schedl and Y. V. Shidlovskii},
title = {The 3′UTR of the Drosophila CPEB translation factor gene orb2 plays a crucial role in spermatogenesis},
journal = {Development (Cambridge)},
year = {2021},
volume = {148},
publisher = {The Company of Biologists},
month = {sep},
url = {https://doi.org/10.1242%2Fdev.198788},
number = {17},
doi = {10.1242/dev.198788}
}