Open Access
PLoS ONE, volume 9, issue 3, pages e89312
Metabolomic Analysis of Three Mollicute Species
Publication type: Journal Article
Publication date: 2014-03-04
Multidisciplinary
Abstract
We present a systematic study of three bacterial species that belong to the class Mollicutes, the smallest and simplest bacteria, Spiroplasma melliferum, Mycoplasma gallisepticum, and Acholeplasma laidlawii. To understand the difference in the basic principles of metabolism regulation and adaptation to environmental conditions in the three species, we analyzed the metabolome of these bacteria. Metabolic pathways were reconstructed using the proteogenomic annotation data provided by our lab. The results of metabolome, proteome and genome profiling suggest a fundamental difference in the adaptation of the three closely related Mollicute species to stress conditions. As the transaldolase is not annotated in Mollicutes, we propose variants of the pentose phosphate pathway catalyzed by annotated enzymes for three species. For metabolite detection we employed high performance liquid chromatography coupled with mass spectrometry. We used liquid chromatography method - hydrophilic interaction chromatography with silica column - as it effectively separates highly polar cellular metabolites prior to their detection by mass spectrometer.
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1 publication, 5.26%
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1 publication, 5.26%
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Springer Nature
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Springer Nature
8 publications, 42.11%
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Frontiers Media S.A.
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3 publications, 15.79%
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Elsevier
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Vanyushkina A. A. et al. Metabolomic Analysis of Three Mollicute Species // PLoS ONE. 2014. Vol. 9. No. 3. p. e89312.
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Vanyushkina A. A., Fisunov G. Y., Gorbachev A. Y., Kamashev D. E., GOVORUN V. M. Metabolomic Analysis of Three Mollicute Species // PLoS ONE. 2014. Vol. 9. No. 3. p. e89312.
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TY - JOUR
DO - 10.1371/journal.pone.0089312
UR - https://doi.org/10.1371%2Fjournal.pone.0089312
TI - Metabolomic Analysis of Three Mollicute Species
T2 - PLoS ONE
AU - Vanyushkina, Anna A
AU - Fisunov, Gleb Y
AU - Gorbachev, Alexey Y
AU - Kamashev, Dmitri E
AU - GOVORUN, VADIM M.
PY - 2014
DA - 2014/03/04 00:00:00
PB - Public Library of Science (PLoS)
SP - e89312
IS - 3
VL - 9
SN - 1932-6203
ER -
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@article{2014_Vanyushkina,
author = {Anna A Vanyushkina and Gleb Y Fisunov and Alexey Y Gorbachev and Dmitri E Kamashev and VADIM M. GOVORUN},
title = {Metabolomic Analysis of Three Mollicute Species},
journal = {PLoS ONE},
year = {2014},
volume = {9},
publisher = {Public Library of Science (PLoS)},
month = {mar},
url = {https://doi.org/10.1371%2Fjournal.pone.0089312},
number = {3},
pages = {e89312},
doi = {10.1371/journal.pone.0089312}
}
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Vanyushkina, Anna A., et al. “Metabolomic Analysis of Three Mollicute Species.” PLoS ONE, vol. 9, no. 3, Mar. 2014, p. e89312. https://doi.org/10.1371%2Fjournal.pone.0089312.
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