Laboratory of Proteomic analysis
Publications
22
Citations
418
h-index
10
Authorization required.
Investigation of the interaction of bacterial and eukaryotic cells ("pathogen-host") to search for and clarify the fundamental mechanisms of pathogenesis of multifactorial diseases.
- Next Generation Sequencing (NGS)
- MALDI mass spectroscopy
- Real-time PCR and digital PCR, including design and validation of primers and probes to your target
- Cloning
- Working with bacterial strains
- Oxford Nanopore Technologies
- Sequencing by Sanger
- Gel electrophoresis
- Mass spectrometry
Mariya Galyamina
Junior researcher
Research directions
Study of the molecular mechanisms of long-term epigenetic memory in the adhesive-invasive form of E. coli (AIEC)
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This study is aimed at studying the molecular mechanisms of epigenetic memory in the adhesive-invasive form of Escherichia coli (AIEC). Epigenetic memory is defined as the ability of an organism to register previous events at the level of gene expression without changing the DNA sequence. This phenomenon plays a significant role in the progression of many diseases, including inflammatory bowel diseases. The adhesive-invasive form of Escherichia coli (AIEC) is a bacterium significantly present in patients suffering from Crohn's disease. This microorganism has the ability to penetrate into intestinal cells and initiate an inflammatory process. In the context of the ongoing study, scientists are trying to figure out how AIEC "registers" previous interactions with the host immune system and the intestinal environment, and how this affects its ability to initiate inflammation. This may include analysis of various molecular mechanisms such as DNA modifications, changes in chromatin structure, and regulation of gene expression. Awareness of these mechanisms may contribute to the development of new strategies for the treatment of inflammatory bowel diseases such as Crohn's disease. This may involve the creation of drugs that affect the epigenetic memory of AIEC, or strategies that enhance the effectiveness of the immune system in the fight against this bacterium.
Publications and patents
2023
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2024
| Галямина Мария Алексеевна
Lab address
Москва, Малая Пироговская ул., 1, стр. 3
Authorization required.