Open Access
Ellagic Acid Derivatives from Terminalia chebula Retz. Downregulate the Expression of Quorum Sensing Genes to Attenuate Pseudomonas aeruginosa PAO1 Virulence
Publication type: Journal Article
Publication date: 2013-01-08
scimago Q1
wos Q2
SJR: 0.803
CiteScore: 5.4
Impact factor: 2.6
ISSN: 19326203
PubMed ID:
23320085
Multidisciplinary
Abstract
Background Burgeoning antibiotic resistance in Pseudomonas aeruginosa has necessitated the development of anti pathogenic agents that can quench acylhomoserine lactone (AHL) mediated QS with least risk of resistance. This study explores the anti quorum sensing potential of T. chebula Retz. and identification of probable compounds(s) showing anti QS activity and the mechanism of attenuation of P. aeruginosa PAO1 virulence factors. Methods and Results Methanol extract of T. chebula Retz. fruit showed anti QS activity using Agrobacterium tumefaciens A136. Bioactive fraction (F7), obtained by fractionation of methanol extract using Sephadex LH20, showed significant reduction (p<0.001) in QS regulated production of extracellular virulence factors in P. aeruginosa PAO1. Biofilm formation and alginate were significantly (p<0.05) reduced with enhanced (20%) susceptibility to tobramycin. Real Time PCR of F7 treated P. aeruginosa showed down regulation of autoinducer synthase (lasI and rhlI) and their cognate receptor (lasR and rhlR) genes by 89, 90, 90 and 93%, respectively. Electrospray Ionization Mass Spectrometry also showed 90 and 64% reduction in the production of 3-oxo-C12HSL and C4HSL after treatment. Decrease in AHLs as one of the mechanisms of quorum quenching by F7 was supported by the reversal of inhibited swarming motility in F7-treated P. aeruginosa PAO1 on addition of C4HSL. F7 also showed antagonistic activity against 3-oxo-C12HSL-dependent QS in E. coli bioreporter. C. elegans fed on F7-treated P. aeruginosa showed enhanced survival with LT50 increasing from 24 to 72 h. LC-ESI-MS of F7 revealed the presence of ellagic acid derivatives responsible for anti QS activity in T. chebula extract. Conclusions This is the first report on anti QS activity of T. chebula fruit linked to EADs which down regulate the expression of lasIR and rhlIR genes with concomitant decrease in AHLs in P. aeruginosa PAO1 causing attenuation of its virulence factors and enhanced sensitivity of its biofilm towards tobramycin.
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Sarabhai S., Sharma P., CAPALASH N. Ellagic Acid Derivatives from Terminalia chebula Retz. Downregulate the Expression of Quorum Sensing Genes to Attenuate Pseudomonas aeruginosa PAO1 Virulence // PLoS ONE. 2013. Vol. 8. No. 1. p. e53441.
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Sarabhai S., Sharma P., CAPALASH N. Ellagic Acid Derivatives from Terminalia chebula Retz. Downregulate the Expression of Quorum Sensing Genes to Attenuate Pseudomonas aeruginosa PAO1 Virulence // PLoS ONE. 2013. Vol. 8. No. 1. p. e53441.
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TY - JOUR
DO - 10.1371/journal.pone.0053441
UR - https://doi.org/10.1371/journal.pone.0053441
TI - Ellagic Acid Derivatives from Terminalia chebula Retz. Downregulate the Expression of Quorum Sensing Genes to Attenuate Pseudomonas aeruginosa PAO1 Virulence
T2 - PLoS ONE
AU - Sarabhai, Sajal
AU - Sharma, Prince
AU - CAPALASH, NEENA
PY - 2013
DA - 2013/01/08
PB - Public Library of Science (PLoS)
SP - e53441
IS - 1
VL - 8
PMID - 23320085
SN - 1932-6203
ER -
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@article{2013_Sarabhai,
author = {Sajal Sarabhai and Prince Sharma and NEENA CAPALASH},
title = {Ellagic Acid Derivatives from Terminalia chebula Retz. Downregulate the Expression of Quorum Sensing Genes to Attenuate Pseudomonas aeruginosa PAO1 Virulence},
journal = {PLoS ONE},
year = {2013},
volume = {8},
publisher = {Public Library of Science (PLoS)},
month = {jan},
url = {https://doi.org/10.1371/journal.pone.0053441},
number = {1},
pages = {e53441},
doi = {10.1371/journal.pone.0053441}
}
Cite this
MLA
Copy
Sarabhai, Sajal, et al. “Ellagic Acid Derivatives from Terminalia chebula Retz. Downregulate the Expression of Quorum Sensing Genes to Attenuate Pseudomonas aeruginosa PAO1 Virulence.” PLoS ONE, vol. 8, no. 1, Jan. 2013, p. e53441. https://doi.org/10.1371/journal.pone.0053441.