Open Access
[Overexpression of DNA-methyltransferases in persistency of cccDNA pool in chronic hepatitis B].
D S Kostyushev
1
,
A P Zueva
2, 3
,
Sergey Brezgin
2, 4
,
A D Lipatnikov
2, 5
,
V N Simirskii
6
,
Dieter Glebe
7
,
E V Volchkova
4
,
G A Shipulin
1
,
V P Chulanov
2, 4
Тип публикации: Journal Article
Дата публикации: 2017-11-15
Включен в RSCI
scimago Q2
wos Q4
БС2
SJR: 0.200
CiteScore: 1.5
Impact factor: 0.3
ISSN: 00403660, 23095342
PubMed ID:
29260742
General Medicine
Endocrinology, Diabetes and Metabolism
History
Family Practice
Краткое описание
AIM
To define the role of DNA-methyltransferases of type 1 and type 3A in hepatitis B viral cycle.
MATERIAL AND METHODS
Human hepatoma cells HepG2 with stable expression of 1.1-mer HBV genome were transfected with vectors encoding DNA-methyltransferase 1 (DNMT1), DNA-methyltransferase 3A (DNMT3A) or were co-transfected with these vectors. Total HBV DNA copy number, relative expression of pregenomic RNA (pgRNA), S-protein-encoding RNA (S-RNA) and cccDNA were analyzed by quantitative and semi-quantitative real-time PCR-analysis with TaqMan probes for assessment of DNMTs-mediated effects on HBV.
RESULTS
DNMT1 and DNMT3A suppress HBV transcription and replication, though to different magnitude. cccDNA pool is enlarged statistically significantly ≈2-fold (P<0.005) after transfection of DNMT3A, but is unaltered under DNMT1 treatment.
CONCLUSION
DNMT3A regulates the size of cccDNA pool and is important for persistency of HBV infection.
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ГОСТ
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Kostyushev D. S. et al. [Overexpression of DNA-methyltransferases in persistency of cccDNA pool in chronic hepatitis B]. // Terapevticheskii Arkhiv. 2017. Vol. 89. No. 11. pp. 21-26.
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Kostyushev D. S., Zueva A. P., Brezgin S., Lipatnikov A. D., Simirskii V. N., Glebe D., Volchkova E. V., Shipulin G. A., Chulanov V. P. [Overexpression of DNA-methyltransferases in persistency of cccDNA pool in chronic hepatitis B]. // Terapevticheskii Arkhiv. 2017. Vol. 89. No. 11. pp. 21-26.
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RIS
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TY - JOUR
DO - 10.17116/terarkh2017891121-26
UR - https://doi.org/10.17116/terarkh2017891121-26
TI - [Overexpression of DNA-methyltransferases in persistency of cccDNA pool in chronic hepatitis B].
T2 - Terapevticheskii Arkhiv
AU - Kostyushev, D S
AU - Zueva, A P
AU - Brezgin, Sergey
AU - Lipatnikov, A D
AU - Simirskii, V N
AU - Glebe, Dieter
AU - Volchkova, E V
AU - Shipulin, G A
AU - Chulanov, V P
PY - 2017
DA - 2017/11/15
PB - Consilium Medicum
SP - 21-26
IS - 11
VL - 89
PMID - 29260742
SN - 0040-3660
SN - 2309-5342
ER -
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BibTex (до 50 авторов)
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@article{2017_Kostyushev,
author = {D S Kostyushev and A P Zueva and Sergey Brezgin and A D Lipatnikov and V N Simirskii and Dieter Glebe and E V Volchkova and G A Shipulin and V P Chulanov},
title = {[Overexpression of DNA-methyltransferases in persistency of cccDNA pool in chronic hepatitis B].},
journal = {Terapevticheskii Arkhiv},
year = {2017},
volume = {89},
publisher = {Consilium Medicum},
month = {nov},
url = {https://doi.org/10.17116/terarkh2017891121-26},
number = {11},
pages = {21--26},
doi = {10.17116/terarkh2017891121-26}
}
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MLA
Скопировать
Kostyushev, D. S., et al. “[Overexpression of DNA-methyltransferases in persistency of cccDNA pool in chronic hepatitis B]..” Terapevticheskii Arkhiv, vol. 89, no. 11, Nov. 2017, pp. 21-26. https://doi.org/10.17116/terarkh2017891121-26.
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