Open Access
Open access
Terapevticheskii Arkhiv
,  volume 89 ,  issue 11 ,  pages 21-26

[Overexpression of DNA-methyltransferases in persistency of cccDNA pool in chronic hepatitis B].

Publication type: Journal Article
Publication date: 2017-11-15
scimago Q2
wos Q4
SJR: 0.200
CiteScore: 1.5
Impact factor: 0.3
ISSN: 00403660, 23095342
General Medicine
Endocrinology, Diabetes and Metabolism
History
Family Practice
Abstract
AIM To define the role of DNA-methyltransferases of type 1 and type 3A in hepatitis B viral cycle. MATERIAL AND METHODS Human hepatoma cells HepG2 with stable expression of 1.1-mer HBV genome were transfected with vectors encoding DNA-methyltransferase 1 (DNMT1), DNA-methyltransferase 3A (DNMT3A) or were co-transfected with these vectors. Total HBV DNA copy number, relative expression of pregenomic RNA (pgRNA), S-protein-encoding RNA (S-RNA) and cccDNA were analyzed by quantitative and semi-quantitative real-time PCR-analysis with TaqMan probes for assessment of DNMTs-mediated effects on HBV. RESULTS DNMT1 and DNMT3A suppress HBV transcription and replication, though to different magnitude. cccDNA pool is enlarged statistically significantly ≈2-fold (P<0.005) after transfection of DNMT3A, but is unaltered under DNMT1 treatment. CONCLUSION DNMT3A regulates the size of cccDNA pool and is important for persistency of HBV infection.
Found 
Found 
2 citations
Kostyushev Dmitry
DSc in Biological/biomedical sciences
76 publications,  1 144 citations,  54 reviews
h-index: 18
Sechenov First Moscow State Medical University
Sechenov First Moscow State Medical University
Lomonosov Moscow State University
Lomonosov Moscow State University
Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences
Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences
Research interests
Drug delivery
Gene editing
Infectious Diseases
Small molecules
Stem cells
2 citations
Kostyusheva Anastasiya
🥼 🤝
PhD in Biological/biomedical sciences
43 publications,  888 citations
h-index: 15
Sechenov First Moscow State Medical University
Sechenov First Moscow State Medical University
Research interests
Molecular biology
Virology
2 citations
Brezgin Sergey
🤝
PhD in Biological/biomedical sciences
51 publications,  892 citations,  1 review
h-index: 15
Sechenov First Moscow State Medical University
Sechenov First Moscow State Medical University
Sirius University of Science and Technology
Sirius University of Science and Technology
Research interests
Exosomes
1 citation
Bayurova Ekaterina
PhD in Biological/biomedical sciences
36 publications,  381 citations
h-index: 11
M.P. Chumakov Federal Scientific Center for Research and Development of Immunobiological Drugs of the Russian Academy of Sciences
M.P. Chumakov Federal Scientific Center for Research and Development of Immunobiological Drugs of the Russian Academy of Sciences
Research interests
Molecular Virology
Virology
1 citation
Gordeychuk Ilya
PhD in Health sciences
57 publications,  494 citations,  16 reviews
h-index: 14
M.P. Chumakov Federal Scientific Center for Research and Development of Immunobiological Drugs of the Russian Academy of Sciences
M.P. Chumakov Federal Scientific Center for Research and Development of Immunobiological Drugs of the Russian Academy of Sciences
Research interests
Animal models

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Kostyushev D. S. et al. [Overexpression of DNA-methyltransferases in persistency of cccDNA pool in chronic hepatitis B]. // Terapevticheskii Arkhiv. 2017. Vol. 89. No. 11. pp. 21-26.
GOST all authors (up to 50) Copy
Kostyushev D. S., Zueva A. P., Brezgin S., Lipatnikov A. D., Simirskii V. N., Glebe D., Volchkova E. V., Shipulin G. A., Chulanov V. P. [Overexpression of DNA-methyltransferases in persistency of cccDNA pool in chronic hepatitis B]. // Terapevticheskii Arkhiv. 2017. Vol. 89. No. 11. pp. 21-26.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.17116/terarkh2017891121-26
UR - https://doi.org/10.17116/terarkh2017891121-26
TI - [Overexpression of DNA-methyltransferases in persistency of cccDNA pool in chronic hepatitis B].
T2 - Terapevticheskii Arkhiv
AU - Kostyushev, D S
AU - Zueva, A P
AU - Brezgin, Sergey
AU - Lipatnikov, A D
AU - Simirskii, V N
AU - Glebe, Dieter
AU - Volchkova, E V
AU - Shipulin, G A
AU - Chulanov, V P
PY - 2017
DA - 2017/11/15
PB - Consilium Medicum
SP - 21-26
IS - 11
VL - 89
PMID - 29260742
SN - 0040-3660
SN - 2309-5342
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{2017_Kostyushev,
author = {D S Kostyushev and A P Zueva and Sergey Brezgin and A D Lipatnikov and V N Simirskii and Dieter Glebe and E V Volchkova and G A Shipulin and V P Chulanov},
title = {[Overexpression of DNA-methyltransferases in persistency of cccDNA pool in chronic hepatitis B].},
journal = {Terapevticheskii Arkhiv},
year = {2017},
volume = {89},
publisher = {Consilium Medicum},
month = {nov},
url = {https://doi.org/10.17116/terarkh2017891121-26},
number = {11},
pages = {21--26},
doi = {10.17116/terarkh2017891121-26}
}
MLA
Cite this
MLA Copy
Kostyushev, D. S., et al. “[Overexpression of DNA-methyltransferases in persistency of cccDNA pool in chronic hepatitis B]..” Terapevticheskii Arkhiv, vol. 89, no. 11, Nov. 2017, pp. 21-26. https://doi.org/10.17116/terarkh2017891121-26.