Obtaining and Purification of Recombinant Domain III of Human Alpha-Fetoprotein

Mollaev M.D., Zabolotskii A.I., Mazalev D.A., Gorokhovets N.V., Sokol M.B., Mollaeva M.R., Fomicheva M.V., Pshenichnikova A.B., Nikolskaya E.D.
Тип документа: Journal Article
Дата публикации: 2021-01-24
Название журнала: Biotekhnologiya
Издатель: State Research Institute for Genetics and Selection of Industrial Microorganisms
Квартиль по SCImago: Q4
Квартиль по Web of Science: —
Импакт-фактор 2021: —
ISSN: 02342758, 25002341
DOI:  10.21519/0234-2758-2021-37-4-32-42
Applied Microbiology and Biotechnology
Biotechnology
Ecology
Краткое описание
Previous studies have demonstrated the applicability of alpha-fetoprotein or its receptor-binding domain fused to the 6-histidine tag at the C-terminus (rAFP3D-His6) as vectors for targeted delivery of antitumor agents. This tag is undesirable for further preclinical trials. Therefore, we designed a recombinant protein rAFP3D without any affine tags, and accessed its functional activity. The protein was produced as inclusion bodies in Escherichia coli BL21 (DE3) cells. Optimal conditions for washing inclusion bodies and refolding the target protein were selected. We used a saturated solution of (NH4)2SO4 as the primary purification step to precipitate the main fraction of protein admixtures. The second purification step included hydrophobic chromatography using butyl-cellulose. The identity of the protein sequence was confirmed by tandem mass spectrometry. Circular dichroism demonstrated the authenticity of the secondary structure. Fluorescently labeled rAFP3D actively penetrated into MCF-7 tumor cells. These results indicate that rAFP3D can be used for targeted drug delivery. Key words: alpha-fetoprotein, receptor-binding domain, recombinant protein, MALDI-MS, HPLC, drug delivery system Funding - The project was supported with Russian Foundation for Basic Research (project no. 18-29-09022/20).
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1. Mollaev M.D. и др. Obtaining and Purification of Recombinant Domain III of Human Alpha-Fetoprotein // Biotekhnologiya. 2021. Т. 37. № 4. С. 32–42.
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TY - JOUR

DO - 10.21519/0234-2758-2021-37-4-32-42

UR - http://dx.doi.org/10.21519/0234-2758-2021-37-4-32-42

TI - Obtaining and Purification of Recombinant Domain III of Human Alpha-Fetoprotein

T2 - Biotekhnologiya

AU - Mollaev, M.D.

AU - Zabolotskii, A.I.

AU - Mazalev, D.A.

AU - Gorokhovets, N.V.

AU - Sokol, M.B.

AU - Mollaeva, M.R.

AU - Fomicheva, M.V.

AU - Pshenichnikova, A.B.

AU - Nikolskaya, E.D.

PY - 2021

PB - National Research Center Kurchatov Institute

SP - 32-42

IS - 4

VL - 37

SN - 2500-2341

SN - 0234-2758

ER -

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@article{Mollaev_2021,

doi = {10.21519/0234-2758-2021-37-4-32-42},

url = {https://doi.org/10.21519%2F0234-2758-2021-37-4-32-42},

year = 2021,

publisher = {National Research Center Kurchatov Institute},

volume = {37},

number = {4},

pages = {32--42},

author = {M.D. Mollaev and A.I. Zabolotskii and D.A. Mazalev and N.V. Gorokhovets and M.B. Sokol and M.R. Mollaeva and M.V. Fomicheva and A.B. Pshenichnikova and E.D. Nikolskaya},

title = {Obtaining and Purification of Recombinant Domain {III} of Human Alpha-Fetoprotein},

journal = {Biotekhnologiya}

}

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Mollaev, M.D. et al. “Obtaining and Purification of Recombinant Domain III of Human Alpha-Fetoprotein.” Biotekhnologiya 37.4 (2021): 32–42. Crossref. Web.