Bone marrow cell morphology in congenital diserythropoietic anemia: selective enrichment of the studied cell population for light and electron microscopy using a microarray and centrifugation in a density gradient

Congenital diserythropoietic anemias are a group of rare inherited diseases causing uneffective erythropoiesis and multiple morphological changes in erythroblasts. Their differential diagnosis involves a study of the morphology of minor bone marrow cell populations that requires a selective cell enrichment. Here we describe two methods of the population enrichment by erythroid cells (including the self-developed cell-binding microarray) for their subsequent study by methods of light and electron microscopy.