Moscow University Biological Sciences Bulletin, volume 74, issue 3, pages 163-169
Studies into the Effect of “Mild” Uncoupling with 2,4-Dinitrophenol on the Growth of Chinese Hamster Cell Culture and Its Subsequent Dying out in the Stationary Phase
Publication type: Journal Article
Publication date: 2019-07-01
Quartile SCImago
Q4
Quartile WOS
—
Impact factor: —
ISSN: 00963925, 1934791X
General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences
General Environmental Science
Abstract
Partial uncoupling of the processes of oxidative phosphorylation and energy storage in the form of ATP (“mild” uncoupling) helps reduce the production of reactive oxygen species and can also mimic the effect of calorie restriction. A number of studies have shown that uncouplers, such as 2,4-dinitrophenol (DNP), affect the lifespan of Drosophila, yeast, mice, and rats as well as the manifestation of “age-related” changes in cultures of mammalian and human cells undergoing replicative senescence. This paper is devoted to studying the effect of DNP on the growth and subsequent dying out of “stationary phase aging” Chinese hamster cells. Using the method for evaluating the colony-forming efficiency of cells, the maximum permissible concentration was selected, 5 ×10–5 M, in which the substance presumably induces “mild” uncoupling and does not inhibit cell proliferation. At higher concentrations, DNP has a cytotoxic effect on the studied cell culture. Under the influence of DNP in the potentially “mild” uncoupling concentration (5.6 × 10–7 M), the kinetics of cell growth and dying out does not change, and the lifespan of the cell culture does not increase. This effect may be due to the type of cells studied. In addition, there is a probability that the optimal concentration lies in the range from 5 ×10–7 to 5 × 10–5 M or even lower than 5 × 10–7 M.
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Pleiades Publishing
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6 publications, 85.71%
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1 publication, 14.29%
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Morgunova G. V. et al. Studies into the Effect of “Mild” Uncoupling with 2,4-Dinitrophenol on the Growth of Chinese Hamster Cell Culture and Its Subsequent Dying out in the Stationary Phase // Moscow University Biological Sciences Bulletin. 2019. Vol. 74. No. 3. pp. 163-169.
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Morgunova G. V., Karmushakov A. F., Klebanov A. A., Khokhlov A. N. Studies into the Effect of “Mild” Uncoupling with 2,4-Dinitrophenol on the Growth of Chinese Hamster Cell Culture and Its Subsequent Dying out in the Stationary Phase // Moscow University Biological Sciences Bulletin. 2019. Vol. 74. No. 3. pp. 163-169.
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TY - JOUR
DO - 10.3103/S0096392519030088
UR - https://doi.org/10.3103%2FS0096392519030088
TI - Studies into the Effect of “Mild” Uncoupling with 2,4-Dinitrophenol on the Growth of Chinese Hamster Cell Culture and Its Subsequent Dying out in the Stationary Phase
T2 - Moscow University Biological Sciences Bulletin
AU - Karmushakov, A F
AU - Klebanov, A A
AU - Morgunova, G V
AU - Khokhlov, A N
PY - 2019
DA - 2019/07/01 00:00:00
PB - Pleiades Publishing
SP - 163-169
IS - 3
VL - 74
SN - 0096-3925
SN - 1934-791X
ER -
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@article{2019_Morgunova,
author = {A F Karmushakov and A A Klebanov and G V Morgunova and A N Khokhlov},
title = {Studies into the Effect of “Mild” Uncoupling with 2,4-Dinitrophenol on the Growth of Chinese Hamster Cell Culture and Its Subsequent Dying out in the Stationary Phase},
journal = {Moscow University Biological Sciences Bulletin},
year = {2019},
volume = {74},
publisher = {Pleiades Publishing},
month = {jul},
url = {https://doi.org/10.3103%2FS0096392519030088},
number = {3},
pages = {163--169},
doi = {10.3103/S0096392519030088}
}
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Morgunova, G. V., et al. “Studies into the Effect of “Mild” Uncoupling with 2,4-Dinitrophenol on the Growth of Chinese Hamster Cell Culture and Its Subsequent Dying out in the Stationary Phase.” Moscow University Biological Sciences Bulletin, vol. 74, no. 3, Jul. 2019, pp. 163-169. https://doi.org/10.3103%2FS0096392519030088.
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