Moscow University Chemistry Bulletin, volume 65, issue 3, pages 165-169
Optimized Detection Method of Telomerase Activity in Cancer Diagnostics
Skvortsov D A
1
,
Zvereva M E
1
,
Rubtsova M. P.
1
,
Pavlova L S
2
,
Petrenko A A
2
,
Kisseljov F L
2
,
Publication type: Journal Article
Publication date: 2010-06-01
Journal:
Moscow University Chemistry Bulletin
Quartile SCImago
Q4
Quartile WOS
—
Impact factor: 0.6
ISSN: 00271314, 19350260
General Chemistry
Abstract
Telomerase is a ribonucleoprotein complex; it uses an internal RNA template to synthesize telomere DNA. Telomerase is active in 90% of cancers and can be used as a diagnostic marker. We have optimized conditions for the extraction from small tissue samples (<0.05 g) of cervical lesions to analyze telomerase activity and selected the optimal concentrations of the tissue extracts. Different concentrations of the extracts were used to determine the presence of possible telomerase inhibitors and Taq-polymerase in the extracts. Using lung and kidney cancer samples it was shown that these conditions are applicable for the estimation of telomerase activity in different cancer types. Many investigations of telomerase activity using different types of TRAP (Telomere Repeat Amplification Protocol) have been performed. The possibility of comparison of TRAP results with radioactive and Sybr green detection remains open. We compared these two types of detection for several samples of cervical intraepithelial neoplasias and conclude that they have similar sensitivities.
Citations by journals
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Biochemistry (Moscow)
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Biochemistry (Moscow)
1 publication, 100%
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Citations by publishers
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Pleiades Publishing
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Pleiades Publishing
1 publication, 100%
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Skvortsov D. A. et al. Optimized Detection Method of Telomerase Activity in Cancer Diagnostics // Moscow University Chemistry Bulletin. 2010. Vol. 65. No. 3. pp. 165-169.
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Skvortsov D. A., Zvereva M. E., Rubtsova M. P., Pavlova L. S., Petrenko A. A., Kisseljov F. L., Dontsova O. A. Optimized Detection Method of Telomerase Activity in Cancer Diagnostics // Moscow University Chemistry Bulletin. 2010. Vol. 65. No. 3. pp. 165-169.
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TY - JOUR
DO - 10.3103/S0027131410030119
UR - https://doi.org/10.3103%2FS0027131410030119
TI - Optimized Detection Method of Telomerase Activity in Cancer Diagnostics
T2 - Moscow University Chemistry Bulletin
AU - Skvortsov, D A
AU - Zvereva, M E
AU - Rubtsova, M. P.
AU - Pavlova, L S
AU - Petrenko, A A
AU - Kisseljov, F L
AU - Dontsova, O. A.
PY - 2010
DA - 2010/06/01 00:00:00
PB - Pleiades Publishing
SP - 165-169
IS - 3
VL - 65
SN - 0027-1314
SN - 1935-0260
ER -
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@article{2010_Skvortsov,
author = {D A Skvortsov and M E Zvereva and M. P. Rubtsova and L S Pavlova and A A Petrenko and F L Kisseljov and O. A. Dontsova},
title = {Optimized Detection Method of Telomerase Activity in Cancer Diagnostics},
journal = {Moscow University Chemistry Bulletin},
year = {2010},
volume = {65},
publisher = {Pleiades Publishing},
month = {jun},
url = {https://doi.org/10.3103%2FS0027131410030119},
number = {3},
pages = {165--169},
doi = {10.3103/S0027131410030119}
}
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MLA
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Skvortsov, D. A., et al. “Optimized Detection Method of Telomerase Activity in Cancer Diagnostics.” Moscow University Chemistry Bulletin, vol. 65, no. 3, Jun. 2010, pp. 165-169. https://doi.org/10.3103%2FS0027131410030119.