Open Access
Frontiers in Cardiovascular Medicine, volume 9
Multi-omics of in vitro aortic valve calcification
Semenova Daria
1, 2
,
Zabirnyk Arsenii
3, 4
,
Lobov Arseniy
2
,
Boyarskaya Nadezda
1
,
Kachanova Olga
1
,
Uspensky Vladimir
1
,
Zainullina Bozhana
5
,
Denisov Evgeny
6
,
Gerashchenko Tatiana
6
,
Kvitting John-Peder Escobar
3, 4
,
Kaljusto Mari Liis
3
,
Thiede Bernd
4
,
Kostareva Anna
1
,
Stensløkken Kåre-Olav
4
,
Vaage Jarle
3, 4
,
Publication type: Journal Article
Publication date: 2022-11-03
Journal:
Frontiers in Cardiovascular Medicine
Quartile SCImago
Q1
Quartile WOS
Q2
Impact factor: 3.6
ISSN: 2297055X
PubMed ID:
36407442
Cardiology and Cardiovascular Medicine
Abstract
Heart valve calcification is an active cellular and molecular process that partly remains unknown. Osteogenic differentiation of valve interstitial cells (VIC) is a central mechanism in calcific aortic valve disease (CAVD). Studying mechanisms in CAVD progression is clearly needed. In this study, we compared molecular mechanisms of osteogenic differentiation of human VIC isolated from healthy donors or patients with CAVD by RNA-seq transcriptomics in early timepoint (48 h) and by shotgun proteomics at later timepoint (10th day). Bioinformatic analysis revealed genes and pathways involved in the regulation of VIC osteogenic differentiation. We found a high amount of stage-specific differentially expressed genes and good accordance between transcriptomic and proteomic data. Functional annotation of differentially expressed proteins revealed that osteogenic differentiation of VIC involved many signaling cascades such as: PI3K-Akt, MAPK, Ras, TNF signaling pathways. Wnt, FoxO, and HIF-1 signaling pathways were modulated only at the early timepoint and thus probably involved in the commitment of VIC to osteogenic differentiation. We also observed a significant shift of some metabolic pathways in the early stage of VIC osteogenic differentiation. Lentiviral overexpression of one of the most upregulated genes (ZBTB16, PLZF) increased calcification of VIC after osteogenic stimulation. Analysis with qPCR and shotgun proteomics suggested a proosteogenic role of ZBTB16 in the early stages of osteogenic differentiation.
Citations by journals
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1
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Frontiers in Cardiovascular Medicine
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1 publication, 16.67%
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1 publication, 16.67%
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Russian Journal for Personalized Medicine
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International Journal of Molecular Sciences
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1 publication, 16.67%
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Journal of Cardiovascular Development and Disease
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1 publication, 16.67%
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Basic Research in Cardiology
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1 publication, 16.67%
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1
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Citations by publishers
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1
2
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
2 publications, 33.33%
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Frontiers Media S.A.
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Frontiers Media S.A.
1 publication, 16.67%
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Saint Petersburg State University
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Saint Petersburg State University
1 publication, 16.67%
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Almazov National Medical Research Centre
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Almazov National Medical Research Centre
1 publication, 16.67%
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Springer Nature
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Springer Nature
1 publication, 16.67%
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1
2
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Semenova D. et al. Multi-omics of in vitro aortic valve calcification // Frontiers in Cardiovascular Medicine. 2022. Vol. 9.
GOST all authors (up to 50)
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Semenova D., Zabirnyk A., Lobov A., Boyarskaya N., Kachanova O., Uspensky V., Zainullina B., Denisov E., Gerashchenko T., Kvitting J. E., Kaljusto M. L., Thiede B., Kostareva A., Stensløkken K., Vaage J., Malashicheva A. Multi-omics of in vitro aortic valve calcification // Frontiers in Cardiovascular Medicine. 2022. Vol. 9.
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TY - JOUR
DO - 10.3389/fcvm.2022.1043165
UR - https://doi.org/10.3389%2Ffcvm.2022.1043165
TI - Multi-omics of in vitro aortic valve calcification
T2 - Frontiers in Cardiovascular Medicine
AU - Semenova, Daria
AU - Zabirnyk, Arsenii
AU - Lobov, Arseniy
AU - Boyarskaya, Nadezda
AU - Kachanova, Olga
AU - Uspensky, Vladimir
AU - Zainullina, Bozhana
AU - Denisov, Evgeny
AU - Gerashchenko, Tatiana
AU - Kvitting, John-Peder Escobar
AU - Kaljusto, Mari Liis
AU - Thiede, Bernd
AU - Kostareva, Anna
AU - Stensløkken, Kåre-Olav
AU - Vaage, Jarle
AU - Malashicheva, Anna
PY - 2022
DA - 2022/11/03 00:00:00
PB - Frontiers Media S.A.
VL - 9
PMID - 36407442
SN - 2297-055X
ER -
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@article{2022_Semenova,
author = {Daria Semenova and Arsenii Zabirnyk and Arseniy Lobov and Nadezda Boyarskaya and Olga Kachanova and Vladimir Uspensky and Bozhana Zainullina and Evgeny Denisov and Tatiana Gerashchenko and John-Peder Escobar Kvitting and Mari Liis Kaljusto and Bernd Thiede and Anna Kostareva and Kåre-Olav Stensløkken and Jarle Vaage and Anna Malashicheva},
title = {Multi-omics of in vitro aortic valve calcification},
journal = {Frontiers in Cardiovascular Medicine},
year = {2022},
volume = {9},
publisher = {Frontiers Media S.A.},
month = {nov},
url = {https://doi.org/10.3389%2Ffcvm.2022.1043165},
doi = {10.3389/fcvm.2022.1043165}
}
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