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Frontiers in Microbiology, volume 13

Staphylococcus aureus is able to generate resistance to novel lipoglycopeptide antibiotic gausemycin A

Poshvina Darya V. ¹

Dilbaryan Diana S ¹

Kasyanov Sergey P ²

Sadykova Vera S ³

Lapchinskaya Olda A ³

Rogozhin Eugene A. ^{3, 4}

Vasilchenko Alexey S. ¹

Hide authors affiliations Show authors affiliations: 4 affiliations

Laboratory of Antimicrobial Resistance, Institute of Environmental and Agricultural Biology (X-BIO), Tyumen State University, Russia |

A.V. Zhirmunsky National Scientific Center of Marine Biology, Russia |

Gause Institute of New Antibiotics, Russia |

⁴

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, Russia |

Publication type: Journal Article

Publication date: 2022-09-29

Frontiers Media S.A.

Journal: Frontiers in Microbiology

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Impact factor: 5.2

ISSN: 1664302X

DOI: 10.3389/fmicb.2022.963979

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PubMed ID: 36246291

Microbiology (medical)

Microbiology

Abstract

Gausemycin A is the first member of the novel lipoglycopeptides family produced by Streptomyces roseoflavus INA-Ac-5812. Gausemycin A has a pronounced bactericidal activity against methicillin-resistant Staphylococcus aureus. However, the ability of S. aureus to be resistant to gausemycin A has not been investigated yet. Using serial passaging, we have obtained the resistant variant S. aureus 5812R, which is 80 times more resistant compared to the parent strain. Susceptibility testing of S. aureus 5812R revealed the acquisition of cross-resistance to daptomycin, cefazolin, tetracycline, and gentamicin, while the resistance to vancomycin, nisin, and ramoplanin was absent. Whole genome sequencing revealed single nucleotide polymorphism (SNP) and deletions in S. aureus 5812R, among which are genes encoding efflux pump (sepA), the two-component Kdp system (kdpE), and the component of isoprenoid biosynthesis pathway (hepT). Phenotypically, S. aureus 5812R resembles a small-colony variant, as it is slow-growing, forms small colonies, and is deficient in pigments. Profiling of fatty acids (FA) composition constituting the cytoplasmic membrane of S. aureus 5812R revealed the prevalence of anteiso-branched FA, while straight FA was slightly less present. The evidence also showed that the gausemycin A-resistant strain has increased expression of the cls2 gene of the cardiolipin synthase. The performed checkerboard assay pointed out that the combination of gausemycin A and ciprofloxacin showed a synergistic effect against S. aureus 5812R.

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Microorganisms	Microorganisms, 1, 33.33% Microorganisms 1 publication, 33.33%
Antibiotics	Antibiotics, 1, 33.33% Antibiotics 1 publication, 33.33%
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Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 2, 66.67% Multidisciplinary Digital Publishing Institute (MDPI) 2 publications, 66.67%
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Poshvina D. V. et al. Staphylococcus aureus is able to generate resistance to novel lipoglycopeptide antibiotic gausemycin A // Frontiers in Microbiology. 2022. Vol. 13.

GOST all authors (up to 50) Copy

Poshvina D. V., Dilbaryan D. S., Kasyanov S. P., Sadykova V. S., Lapchinskaya O. A., Rogozhin E. A., Vasilchenko A. S. Staphylococcus aureus is able to generate resistance to novel lipoglycopeptide antibiotic gausemycin A // Frontiers in Microbiology. 2022. Vol. 13.

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RIS Copy

TY - JOUR

DO - 10.3389/fmicb.2022.963979

UR - https://doi.org/10.3389%2Ffmicb.2022.963979

TI - Staphylococcus aureus is able to generate resistance to novel lipoglycopeptide antibiotic gausemycin A

T2 - Frontiers in Microbiology

AU - Poshvina, Darya V.

AU - Dilbaryan, Diana S

AU - Kasyanov, Sergey P

AU - Sadykova, Vera S

AU - Lapchinskaya, Olda A

AU - Rogozhin, Eugene A.

AU - Vasilchenko, Alexey S.

PY - 2022

DA - 2022/09/29 00:00:00

PB - Frontiers Media S.A.

VL - 13

PMID - 36246291

SN - 1664-302X

ER -

BibTex

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BibTex Copy

@article{2022_Poshvina,

author = {Darya V. Poshvina and Diana S Dilbaryan and Sergey P Kasyanov and Vera S Sadykova and Olda A Lapchinskaya and Eugene A. Rogozhin and Alexey S. Vasilchenko},

title = {Staphylococcus aureus is able to generate resistance to novel lipoglycopeptide antibiotic gausemycin A},

journal = {Frontiers in Microbiology},

year = {2022},

volume = {13},

publisher = {Frontiers Media S.A.},

month = {sep},

url = {https://doi.org/10.3389%2Ffmicb.2022.963979},

doi = {10.3389/fmicb.2022.963979}

}

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Publisher

Frontiers Media S.A.

Journal

Frontiers in Microbiology

Quartile SCImago

Quartile WOS

Impact factor

5.2

ISSN

1664302X (Print)

Labs

Antimicrobial Resistance Laboratory

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