Open Access
Frontiers in Genetics, volume 11
Comprehensive Functional Analysis of Escherichia coli Ribosomal RNA Methyltransferases
Pletnev Philipp
1, 2, 3
,
Guseva Ekaterina
4
,
Zanina Anna
1
,
Evfratov Sergey
1
,
Dzama Margarita
4
,
Treshin Vsevolod
1
,
Pogorelskaya Alexandra
4
,
Osterman Ilya
1, 3
,
Golovina Anna
5
,
Rubtsova Maria
1, 3
,
Serebryakova Marina
3, 5
,
Pobeguts Olga V
6
,
GOVORUN VADIM M.
6
,
Bogdanov Alexey A.
1, 5
,
Dontsova Olga A.
1, 2, 3, 5
,
Sergiev Petr V.
1, 3, 5, 7
Publication type: Journal Article
Publication date: 2020-02-27
Journal:
Frontiers in Genetics
Quartile SCImago
Q2
Quartile WOS
Q2
Impact factor: 3.7
ISSN: 16648021
PubMed ID:
32174967
Genetics
Molecular Medicine
Genetics(clinical)
Abstract
Ribosomal RNAs in all organisms are methylated. The functional role of the majority of modified nucleotides is unknown. We systematically questioned the influence of rRNA methylation in Escherichia coli on a number of characteristics of bacterial cells with the help of a set of rRNA methyltransferase (MT) gene knockout strains from the Keio collection. Analysis of ribosomal subunits sedimentation profiles of the knockout strains revealed a surprisingly small number of rRNA MT that significantly affected ribosome assembly. Accumulation of the assembly intermediates was observed only for the rlmE knockout strain whose growth was retarded most significantly among other rRNA MT knockout strains. Accumulation of the 17S rRNA precursor was observed for rsmA(ksgA) knockout cells as well as for cells devoid of functional rsmB and rlmC genes. Significant differences were found among the WT and the majority of rRNA MT knockout strains in their ability to sustain exogenous protein overexpression. While the majority of the rRNA MT knockout strains supported suboptimal reporter gene expression, the strain devoid of the rsmF gene demonstrated a moderate increase in the yield of ectopic gene expression. Comparative 2D protein gel analysis of rRNA MT knockout strains revealed only minor perturbations of the proteome.
Citations by journals
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1
2
3
4
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RNA
|
RNA
4 publications, 14.81%
|
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Microbiology spectrum
|
Microbiology spectrum
2 publications, 7.41%
|
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Biochemistry (Moscow)
|
Biochemistry (Moscow)
1 publication, 3.7%
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Cells
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Cells
1 publication, 3.7%
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Journal of Biological Chemistry
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Journal of Biological Chemistry
1 publication, 3.7%
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International Journal of Molecular Sciences
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International Journal of Molecular Sciences
1 publication, 3.7%
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Biomolecules
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Biomolecules
1 publication, 3.7%
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Genes
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Genes
1 publication, 3.7%
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Frontiers in Microbiology
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Frontiers in Microbiology
1 publication, 3.7%
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Environmental Pollution
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Environmental Pollution
1 publication, 3.7%
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FEBS Letters
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FEBS Letters
1 publication, 3.7%
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ACS Chemical Biology
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ACS Chemical Biology
1 publication, 3.7%
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Nucleic Acids Research
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Nucleic Acids Research
1 publication, 3.7%
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Bioinformatics
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Bioinformatics
1 publication, 3.7%
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Nature Structural and Molecular Biology
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Nature Structural and Molecular Biology
1 publication, 3.7%
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PLoS Pathogens
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PLoS Pathogens
1 publication, 3.7%
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1
2
3
4
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Citations by publishers
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1
2
3
4
5
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Cold Spring Harbor Laboratory
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Cold Spring Harbor Laboratory
5 publications, 18.52%
|
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Multidisciplinary Digital Publishing Institute (MDPI)
|
Multidisciplinary Digital Publishing Institute (MDPI)
4 publications, 14.81%
|
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American Society for Microbiology
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American Society for Microbiology
2 publications, 7.41%
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Oxford University Press
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Oxford University Press
2 publications, 7.41%
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Pleiades Publishing
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Pleiades Publishing
1 publication, 3.7%
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American Society for Biochemistry and Molecular Biology
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American Society for Biochemistry and Molecular Biology
1 publication, 3.7%
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Frontiers Media S.A.
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Frontiers Media S.A.
1 publication, 3.7%
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Elsevier
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Elsevier
1 publication, 3.7%
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Wiley
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Wiley
1 publication, 3.7%
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American Chemical Society (ACS)
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American Chemical Society (ACS)
1 publication, 3.7%
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Springer Nature
|
Springer Nature
1 publication, 3.7%
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Public Library of Science (PLoS)
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Public Library of Science (PLoS)
1 publication, 3.7%
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1
2
3
4
5
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Pletnev P. et al. Comprehensive Functional Analysis of Escherichia coli Ribosomal RNA Methyltransferases // Frontiers in Genetics. 2020. Vol. 11.
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Pletnev P., Guseva E., Zanina A., Evfratov S., Dzama M., Treshin V., Pogorelskaya A., Osterman I., Golovina A., Rubtsova M., Serebryakova M., Pobeguts O. V., GOVORUN V. M., Bogdanov A. A., Dontsova O. A., Sergiev P. V. Comprehensive Functional Analysis of Escherichia coli Ribosomal RNA Methyltransferases // Frontiers in Genetics. 2020. Vol. 11.
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TY - JOUR
DO - 10.3389/fgene.2020.00097
UR - https://doi.org/10.3389%2Ffgene.2020.00097
TI - Comprehensive Functional Analysis of Escherichia coli Ribosomal RNA Methyltransferases
T2 - Frontiers in Genetics
AU - Pletnev, Philipp
AU - Guseva, Ekaterina
AU - Zanina, Anna
AU - Evfratov, Sergey
AU - Dzama, Margarita
AU - Treshin, Vsevolod
AU - Pogorelskaya, Alexandra
AU - Osterman, Ilya
AU - Golovina, Anna
AU - Rubtsova, Maria
AU - Serebryakova, Marina
AU - Pobeguts, Olga V
AU - GOVORUN, VADIM M.
AU - Bogdanov, Alexey A.
AU - Dontsova, Olga A.
AU - Sergiev, Petr V.
PY - 2020
DA - 2020/02/27 00:00:00
PB - Frontiers Media S.A.
VL - 11
PMID - 32174967
SN - 1664-8021
ER -
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@article{2020_Pletnev,
author = {Philipp Pletnev and Ekaterina Guseva and Anna Zanina and Sergey Evfratov and Margarita Dzama and Vsevolod Treshin and Alexandra Pogorelskaya and Ilya Osterman and Anna Golovina and Maria Rubtsova and Marina Serebryakova and Olga V Pobeguts and VADIM M. GOVORUN and Alexey A. Bogdanov and Olga A. Dontsova and Petr V. Sergiev},
title = {Comprehensive Functional Analysis of Escherichia coli Ribosomal RNA Methyltransferases},
journal = {Frontiers in Genetics},
year = {2020},
volume = {11},
publisher = {Frontiers Media S.A.},
month = {feb},
url = {https://doi.org/10.3389%2Ffgene.2020.00097},
doi = {10.3389/fgene.2020.00097}
}