Open Access
Frontiers in Microbiology, volume 11
A Novel Peptide Antibiotic Produced by Streptomyces roseoflavus Strain INA-Ac-5812 With Directed Activity Against Gram-Positive Bacteria
Julian William T
1
,
Lapchinskaya Olda A
2
,
Katrukha Genrikh S
2
,
Sadykova Vera S
2
,
Rogozhin Eugene A.
2, 3
Publication type: Journal Article
Publication date: 2020-09-15
Journal:
Frontiers in Microbiology
Quartile SCImago
Q1
Quartile WOS
Q2
Impact factor: 5.2
ISSN: 1664302X
PubMed ID:
33072016
Microbiology (medical)
Microbiology
Abstract
In this work, we report the isolation and detailed functional characterization for the new non-ribosomally synthesized antibiotic 5812-A/C, which was derived from metabolites of Streptomyces roseoflavus INA-Ac-5812. According to its chemical structure the studied 5812-A/C preliminary is composed of a cyclic peptide part covalently bounded with an arabinose residue. N-terminal amino acid sequencing of the native peptide has identified its partial structure of Leu-Asp-Gly-Ser-Gly and consisting of a Tyr residue that it supposed two-component peptide nature for the molecule studied. However, the structural analysis of the antibiotic complex derived from Streptomyces roseoflavus INA-Ac-5812 is still ongoing. The mechanism of action of 5812-A/C was assessed in comparison with its most related analogue, the lipopeptide antibiotic daptomycin, given the presence in both antimicrobials of an L-kynurenine amino acid residue. The inhibitory activity of 5812-A/C against Gram-positive bacteria including methicillin-resistant strains of S. aureus was similar to daptomycin. The mechanism of action of 5812-A/C was associated with the disruption of membrane integrity, which differs in comparison to daptomycin and is most similar to the antimicrobial membrane-disturbing peptides. However, 5812-A/C demonstrated a calcium-dependent mode of action. In addition, unlike daptomycin, 5812-A/C was able to penetrate mature biofilms and inhibit the metabolic activity of embedded S. aureus cells. At the same time, 5812-A/C has no hemolytic activity toward erythrocyte but possessed weak cytotoxic activity represented by heterochromatin condensation in human buccal epithelium cells. The biological properties of the peptide 5812-A/C suggest its classification as a calcium-dependent antibiotic effective against a wide spectrum of Gram-positive pathogenic bacteria.
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Citations by publishers
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4 publications, 23.53%
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Springer Nature
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1 publication, 5.88%
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Vasilchenko A. S. et al. A Novel Peptide Antibiotic Produced by Streptomyces roseoflavus Strain INA-Ac-5812 With Directed Activity Against Gram-Positive Bacteria // Frontiers in Microbiology. 2020. Vol. 11.
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Vasilchenko A. S., Julian W. T., Lapchinskaya O. A., Katrukha G. S., Sadykova V. S., Rogozhin E. A. A Novel Peptide Antibiotic Produced by Streptomyces roseoflavus Strain INA-Ac-5812 With Directed Activity Against Gram-Positive Bacteria // Frontiers in Microbiology. 2020. Vol. 11.
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TY - JOUR
DO - 10.3389/fmicb.2020.556063
UR - https://doi.org/10.3389%2Ffmicb.2020.556063
TI - A Novel Peptide Antibiotic Produced by Streptomyces roseoflavus Strain INA-Ac-5812 With Directed Activity Against Gram-Positive Bacteria
T2 - Frontiers in Microbiology
AU - Vasilchenko, Alexey S.
AU - Julian, William T
AU - Lapchinskaya, Olda A
AU - Katrukha, Genrikh S
AU - Sadykova, Vera S
AU - Rogozhin, Eugene A.
PY - 2020
DA - 2020/09/15 00:00:00
PB - Frontiers Media S.A.
VL - 11
PMID - 33072016
SN - 1664-302X
ER -
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@article{2020_Vasilchenko,
author = {Alexey S. Vasilchenko and William T Julian and Olda A Lapchinskaya and Genrikh S Katrukha and Vera S Sadykova and Eugene A. Rogozhin},
title = {A Novel Peptide Antibiotic Produced by Streptomyces roseoflavus Strain INA-Ac-5812 With Directed Activity Against Gram-Positive Bacteria},
journal = {Frontiers in Microbiology},
year = {2020},
volume = {11},
publisher = {Frontiers Media S.A.},
month = {sep},
url = {https://doi.org/10.3389%2Ffmicb.2020.556063},
doi = {10.3389/fmicb.2020.556063}
}
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