Open Access
Biomedicines, volume 10, issue 2, pages 333
Repurposing Based Identification of Novel Inhibitors against MmpS5-MmpL5 Efflux Pump of Mycobacterium smegmatis: A Combined In Silico and In Vitro Study
Mohd. Shahbaaz
1
,
Dmitry A Maslov
2
,
Aleksey A Vatlin
2, 3
,
Valery N. Danilenko
2
,
Maria A Grishina
4
,
Alan Christoffels
1, 5
1
5
Africa Centres for Disease Control and Prevention, African Union Headquarters, Addis Ababa W21K19, Ethiopia
|
Publication type: Journal Article
Publication date: 2022-01-31
General Biochemistry, Genetics and Molecular Biology
Medicine (miscellaneous)
Abstract
In the current era of a pandemic, infections of COVID-19 and Tuberculosis (TB) enhance the detrimental effects of both diseases in suffering individuals. The resistance mechanisms evolving in Mycobacterium tuberculosis are limiting the efficiency of current therapeutic measures and pressurizing the stressed medical infrastructures. The bacterial efflux pumps enable the development of resistance against recently approved drugs such as bedaquiline and clofazimine. Consequently, the MmpS5-MmpL5 protein system was selected because of its role in efflux pumping of anti-TB drugs. The MmpS5-MmpL5 systems of Mycobacterium smegmatis were modelled and the virtual screening was performed using an ASINEX library of 5968 anti-bacterial compounds. The inhibitors with the highest binding affinities and QSAR based highest predicted inhibitory concentration were selected. The MmpS5-MmpL5 associated systems with BDE_26593610 and BDD_27860195 showed highest inhibitory parameters. These were subjected to 100 ns Molecular Dynamics simulations and provided the validation regarding the interaction studies. The in vitro studies demonstrated that the BDE_26593610 and BDD_27860195 can be considered as active inhibitors for M. smegmatis MmpS5-MmpL5. The outcomes of this study can be utilized in other experimentation aimed at drug design and discovery against the drug resistance strains of M. tuberculosis.
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Shahbaaz M. et al. Repurposing Based Identification of Novel Inhibitors against MmpS5-MmpL5 Efflux Pump of Mycobacterium smegmatis: A Combined In Silico and In Vitro Study // Biomedicines. 2022. Vol. 10. No. 2. p. 333.
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Shahbaaz M., Maslov D. A., Vatlin A. A., Danilenko V. N., Grishina M. A., Christoffels A. Repurposing Based Identification of Novel Inhibitors against MmpS5-MmpL5 Efflux Pump of Mycobacterium smegmatis: A Combined In Silico and In Vitro Study // Biomedicines. 2022. Vol. 10. No. 2. p. 333.
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TY - JOUR
DO - 10.3390/biomedicines10020333
UR - https://doi.org/10.3390/biomedicines10020333
TI - Repurposing Based Identification of Novel Inhibitors against MmpS5-MmpL5 Efflux Pump of Mycobacterium smegmatis: A Combined In Silico and In Vitro Study
T2 - Biomedicines
AU - Shahbaaz, Mohd.
AU - Vatlin, Aleksey A
AU - Danilenko, Valery N.
AU - Maslov, Dmitry A
AU - Grishina, Maria A
AU - Christoffels, Alan
PY - 2022
DA - 2022/01/31
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 333
IS - 2
VL - 10
SN - 2227-9059
ER -
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@article{2022_Shahbaaz,
author = {Mohd. Shahbaaz and Aleksey A Vatlin and Valery N. Danilenko and Dmitry A Maslov and Maria A Grishina and Alan Christoffels},
title = {Repurposing Based Identification of Novel Inhibitors against MmpS5-MmpL5 Efflux Pump of Mycobacterium smegmatis: A Combined In Silico and In Vitro Study},
journal = {Biomedicines},
year = {2022},
volume = {10},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {jan},
url = {https://doi.org/10.3390/biomedicines10020333},
number = {2},
pages = {333},
doi = {10.3390/biomedicines10020333}
}
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Shahbaaz, Mohd., et al. “Repurposing Based Identification of Novel Inhibitors against MmpS5-MmpL5 Efflux Pump of Mycobacterium smegmatis: A Combined In Silico and In Vitro Study.” Biomedicines, vol. 10, no. 2, Jan. 2022, p. 333. https://doi.org/10.3390/biomedicines10020333.