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Biomedicines, volume 9, issue 9, pages 1192

Self-Organization Provides Cell Fate Commitment in MSC Sheet Condensed Areas via ROCK-Dependent Mechanism

Nimiritsky Peter ^{1, 2}

Novoseletskaya Ekaterina ^{1, 2}

Eremichev Roman ²

Alexandrushkina Natalia A ^{1, 2}

Karagyaur Maxim ^{1, 2}

Vetrovoy Oleg ^{3, 4}

Basalova Nataliya ^{1, 2}

Khrustaleva Anastasia ⁵

Tyakht Alexander ⁶

Efimenko Anastasia ^{1, 2}

Tkachuk Vsevolod ^{1, 2}

Makarevich Pavel I ^{1, 2}

Hide authors affiliations Show authors affiliations: 6 affiliations

Faculty of Medicine, Lomonosov Moscow State University, 119192 Moscow, Russia |

Institute for Regenerative Medicine, Medical Research and Education Center, Lomonosov Moscow State University, 119192 Moscow, Russia |

Department of Biochemistry, Faculty of Biology, Saint-Petersburg State University, 199034 Saint Petersburg, Russia |

⁴

Laboratory of Regulation of Brain Neuron Functions, Pavlov Institute of Physiology, Russian Academy of Sciences, 199034 Saint Petersburg, Russia |

⁵

Department of the Bioinformatics, Institute of Gene Biology, Russian Academy of Sciences, 119334 Moscow, Russia |

⁶

Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology, Russian Academy of Sciences, 119334 Moscow, Russia |

Publication type: Journal Article

Publication date: 2021-09-10

Multidisciplinary Digital Publishing Institute (MDPI)

Journal: Biomedicines

Quartile SCImago

Quartile WOS

Impact factor: 4.7

ISSN: 22279059

DOI: 10.3390/biomedicines9091192

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General Biochemistry, Genetics and Molecular Biology

Medicine (miscellaneous)

Abstract

Multipotent mesenchymal stem/stromal cells (MSC) are one of the crucial regulators of regeneration and tissue repair and possess an intrinsic program from self-organization mediated by condensation, migration and self-patterning. The ability to self-organize has been successfully exploited in tissue engineering approaches using cell sheets (CS) and their modifications. In this study, we used CS as a model of human MSC spontaneous self-organization to demonstrate its structural, transcriptomic impact and multipotent stromal cell commitment. We used CS formation to visualize MSC self-organization and evaluated the role of the Rho-GTPase pathway in spontaneous condensation, resulting in a significant anisotropy of the cell density within the construct. Differentiation assays were carried out using conventional protocols, and microdissection and RNA-sequencing were applied to establish putative targets behind the observed phenomena. The differentiation of MSC to bone and cartilage, but not to adipocytes in CS, occurred more effectively than in the monolayer. RNA-sequencing indicated transcriptional shifts involving the activation of the Rho-GTPase pathway and repression of SREBP, which was concordant with the lack of adipogenesis in CS. Eventually, we used an inhibitory analysis to validate our findings and suggested a model where the self-organization of MSC defined their commitment and cell fate via ROCK1/2 and SREBP as major effectors under the putative switching control of AMP kinase.

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Citations by journals

	1 2
International Journal of Molecular Sciences	International Journal of Molecular Sciences, 2, 33.33% International Journal of Molecular Sciences 2 publications, 33.33%
Frontiers in Endocrinology	Frontiers in Endocrinology, 1, 16.67% Frontiers in Endocrinology 1 publication, 16.67%
International Journal of Nanomedicine	International Journal of Nanomedicine, 1, 16.67% International Journal of Nanomedicine 1 publication, 16.67%
Chemical Synthesis	Chemical Synthesis, 1, 16.67% Chemical Synthesis 1 publication, 16.67%
Cell Communication and Signaling	Cell Communication and Signaling, 1, 16.67% Cell Communication and Signaling 1 publication, 16.67%
	1 2

Citations by publishers

	1 2
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 2, 33.33% Multidisciplinary Digital Publishing Institute (MDPI) 2 publications, 33.33%
Frontiers Media S.A.	Frontiers Media S.A., 1, 16.67% Frontiers Media S.A. 1 publication, 16.67%
Dove Medical Press	Dove Medical Press, 1, 16.67% Dove Medical Press 1 publication, 16.67%
OAE Publishing Inc.	OAE Publishing Inc., 1, 16.67% OAE Publishing Inc. 1 publication, 16.67%
Springer Nature	Springer Nature, 1, 16.67% Springer Nature 1 publication, 16.67%
	1 2

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Nimiritsky P. et al. Self-Organization Provides Cell Fate Commitment in MSC Sheet Condensed Areas via ROCK-Dependent Mechanism // Biomedicines. 2021. Vol. 9. No. 9. p. 1192.

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Nimiritsky P., Novoseletskaya E., Eremichev R., Alexandrushkina N. A., Karagyaur M., Vetrovoy O., Basalova N., Khrustaleva A., Tyakht A., Efimenko A., Tkachuk V., Makarevich P. I. Self-Organization Provides Cell Fate Commitment in MSC Sheet Condensed Areas via ROCK-Dependent Mechanism // Biomedicines. 2021. Vol. 9. No. 9. p. 1192.

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TY - JOUR

DO - 10.3390/biomedicines9091192

UR - https://doi.org/10.3390%2Fbiomedicines9091192

TI - Self-Organization Provides Cell Fate Commitment in MSC Sheet Condensed Areas via ROCK-Dependent Mechanism

T2 - Biomedicines

AU - Nimiritsky, Peter

AU - Eremichev, Roman

AU - Karagyaur, Maxim

AU - Vetrovoy, Oleg

AU - Basalova, Nataliya

AU - Khrustaleva, Anastasia

AU - Tkachuk, Vsevolod

AU - Novoseletskaya, Ekaterina

AU - Alexandrushkina, Natalia A

AU - Tyakht, Alexander

AU - Efimenko, Anastasia

AU - Makarevich, Pavel I

PY - 2021

DA - 2021/09/10 00:00:00

PB - Multidisciplinary Digital Publishing Institute (MDPI)

SP - 1192

IS - 9

VL - 9

SN - 2227-9059

ER -

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@article{2021_Nimiritsky,

author = {Peter Nimiritsky and Roman Eremichev and Maxim Karagyaur and Oleg Vetrovoy and Nataliya Basalova and Anastasia Khrustaleva and Vsevolod Tkachuk and Ekaterina Novoseletskaya and Natalia A Alexandrushkina and Alexander Tyakht and Anastasia Efimenko and Pavel I Makarevich},

title = {Self-Organization Provides Cell Fate Commitment in MSC Sheet Condensed Areas via ROCK-Dependent Mechanism},

journal = {Biomedicines},

year = {2021},

volume = {9},

publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},

month = {sep},

url = {https://doi.org/10.3390%2Fbiomedicines9091192},

number = {9},

pages = {1192},

doi = {10.3390/biomedicines9091192}

}

MLA

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MLA Copy

Nimiritsky, Peter, et al. “Self-Organization Provides Cell Fate Commitment in MSC Sheet Condensed Areas via ROCK-Dependent Mechanism.” Biomedicines, vol. 9, no. 9, Sep. 2021, p. 1192. https://doi.org/10.3390%2Fbiomedicines9091192.

Found error?

Publisher

Multidisciplinary Digital Publishing Institute (MDPI)

Journal

Biomedicines

Quartile SCImago

Quartile WOS

Impact factor

4.7

ISSN

22279059 (Electronic)

Labs

Laboratory of Gene Cell Therapy

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