Laboratory of Gene Cell Therapy

The human endometrium is renewed in a unique way through regular cycles of hormone-dependent damage and recovery, during which not only rapid wound healing occurs without fibrosis, but also active tissue growth necessary to ensure its receptivity and the realization of reproductive function. In case of violations of this process, there is a whole range of pathological changes associated with thinning of the endometrium, its fibrosis and the formation of adhesions, which leads to infertility and is a risk factor for other diseases of the female reproductive sphere. In this research, the focus is on elucidating the fundamental cellular and molecular mechanisms that regulate endometrial growth during the menstrual cycle. The stromal cells of the endometrium were selected as the object, whose activity determines the course of its recovery, which in modern women occurs up to 300-400 times during reproductive age. The work will use in vitro and in vivo models, as well as methods of molecular biology (including generation of knockouts by target genes using the CRISPR/Cas9 system). Among the targets of particular interest at this stage are estrogen receptors, for which it is shown to be of critical importance in activating stroma growth and, accordingly, ensuring the thickness of the endometrium necessary for its receptivity. During the project, not only the mechanisms of regulation of endometrial regeneration and renewal will be clarified, but also targets for medicinal or biopharmaceutical effects with the aim of correcting the conditions described above will be proposed.