Open Access
Cells, volume 11, issue 8, pages 1369
Taste Cells of the Type III Employ CASR to Maintain Steady Serotonin Exocytosis at Variable Ca2+ in the Extracellular Medium
Cherkashin Aleksandr P
1
,
Rogachevskaja Olga A
1
,
Kabanova Natalia V
1
,
Kotova Polina D
1
,
Bystrova Marina F.
1
,
Publication type: Journal Article
Publication date: 2022-04-18
PubMed ID:
35456048
General Medicine
Abstract
Type III taste cells are the only taste bud cells which express voltage-gated (VG) Ca2+ channels and employ Ca2+-dependent exocytosis to release neurotransmitters, particularly serotonin. The taste bud is a tightly packed cell population, wherein extracellular Ca2+ is expected to fluctuate markedly due to the electrical activity of taste cells. It is currently unclear whether the Ca2+ entry-driven synapse in type III cells could be reliable enough at unsteady extracellular Ca2. Here we assayed depolarization-induced Ca2+ signals and associated serotonin release in isolated type III cells at varied extracellular Ca2+. It turned out that the same depolarizing stimulus elicited invariant Ca2+ signals in type III cells irrespective of bath Ca2+ varied within 0.5–5 mM. The serotonin release from type III cells was assayed with the biosensor approach by using HEK-293 cells co-expressing the recombinant 5-HT4 receptor and genetically encoded cAMP sensor Pink Flamindo. Consistently with the weak Ca2+ dependence of intracellular Ca2+ transients produced by VG Ca2+ entry, depolarization-triggered serotonin secretion varied negligibly with bath Ca2+. The evidence implicated the extracellular Ca2+-sensing receptor in mediating the negative feedback mechanism that regulates VG Ca2+ entry and levels off serotonin release in type III cells at deviating Ca2+ in the extracellular medium.
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Biochemistry and Biophysics Reports
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Biochemistry and Biophysics Reports
1 publication, 25%
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Cells
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Cells
1 publication, 25%
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Cold Spring Harbor Laboratory
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Cold Spring Harbor Laboratory
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Elsevier
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Elsevier
1 publication, 25%
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 25%
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Cherkashin A. P. et al. Taste Cells of the Type III Employ CASR to Maintain Steady Serotonin Exocytosis at Variable Ca2+ in the Extracellular Medium // Cells. 2022. Vol. 11. No. 8. p. 1369.
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Cherkashin A. P., Rogachevskaja O. A., Kabanova N. V., Kotova P. D., Bystrova M. F., Kolesnikov S. S. Taste Cells of the Type III Employ CASR to Maintain Steady Serotonin Exocytosis at Variable Ca2+ in the Extracellular Medium // Cells. 2022. Vol. 11. No. 8. p. 1369.
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TY - JOUR
DO - 10.3390/cells11081369
UR - https://doi.org/10.3390%2Fcells11081369
TI - Taste Cells of the Type III Employ CASR to Maintain Steady Serotonin Exocytosis at Variable Ca2+ in the Extracellular Medium
T2 - Cells
AU - Cherkashin, Aleksandr P
AU - Kotova, Polina D
AU - Bystrova, Marina F.
AU - Kolesnikov, Stanislav S
AU - Rogachevskaja, Olga A
AU - Kabanova, Natalia V
PY - 2022
DA - 2022/04/18 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 1369
IS - 8
VL - 11
PMID - 35456048
SN - 2073-4409
ER -
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@article{2022_Cherkashin,
author = {Aleksandr P Cherkashin and Polina D Kotova and Marina F. Bystrova and Stanislav S Kolesnikov and Olga A Rogachevskaja and Natalia V Kabanova},
title = {Taste Cells of the Type III Employ CASR to Maintain Steady Serotonin Exocytosis at Variable Ca2+ in the Extracellular Medium},
journal = {Cells},
year = {2022},
volume = {11},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {apr},
url = {https://doi.org/10.3390%2Fcells11081369},
number = {8},
pages = {1369},
doi = {10.3390/cells11081369}
}
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Cherkashin, Aleksandr P., et al. “Taste Cells of the Type III Employ CASR to Maintain Steady Serotonin Exocytosis at Variable Ca2+ in the Extracellular Medium.” Cells, vol. 11, no. 8, Apr. 2022, p. 1369. https://doi.org/10.3390%2Fcells11081369.
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