Open Access
Cells, volume 11, issue 12, pages 1888
Synthetic Thymidine Analog Labeling without Misconceptions
Ivanova Anna
1, 2
,
Gruzova Olesya
1
,
Ermolaeva Elizaveta
1
,
Astakhova Olga
1, 2
,
Itaman Sheed
3, 4
,
Enikolopov Grigori
3
,
Lazutkin Alexander A.
1, 2, 3
Publication type: Journal Article
Publication date: 2022-06-10
PubMed ID:
35741018
General Medicine
Abstract
Tagging proliferating cells with thymidine analogs is an indispensable research tool; however, the issue of the potential in vivo cytotoxicity of these compounds remains unresolved. Here, we address these concerns by examining the effects of BrdU and EdU on adult hippocampal neurogenesis and EdU on the perinatal somatic development of mice. We show that, in a wide range of doses, EdU and BrdU label similar numbers of cells in the dentate gyrus shortly after administration. Furthermore, whereas the administration of EdU does not affect the division and survival of neural progenitor within 48 h after injection, it does affect cell survival, as evaluated 6 weeks later. We also show that a single injection of various doses of EdU on the first postnatal day does not lead to noticeable changes in a panel of morphometric criteria within the first week; however, higher doses of EdU adversely affect the subsequent somatic maturation and brain growth of the mouse pups. Our results indicate the potential caveats in labeling the replicating DNA using thymidine analogs and suggest guidelines for applying this approach.
Citations by journals
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1
2
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Cells
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Cells
2 publications, 33.33%
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Cell Proliferation
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1 publication, 16.67%
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Frontiers in Neuroscience
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Frontiers in Neuroscience
1 publication, 16.67%
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Journal of Asthma
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1 publication, 16.67%
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1
2
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Citations by publishers
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1
2
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
2 publications, 33.33%
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Wiley
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Wiley
1 publication, 16.67%
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Frontiers Media S.A.
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Frontiers Media S.A.
1 publication, 16.67%
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Taylor & Francis
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Taylor & Francis
1 publication, 16.67%
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1
2
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Ivanova A. et al. Synthetic Thymidine Analog Labeling without Misconceptions // Cells. 2022. Vol. 11. No. 12. p. 1888.
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Ivanova A., Gruzova O., Ermolaeva E., Astakhova O., Itaman S., Enikolopov G., Lazutkin A. A. Synthetic Thymidine Analog Labeling without Misconceptions // Cells. 2022. Vol. 11. No. 12. p. 1888.
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TY - JOUR
DO - 10.3390/cells11121888
UR - https://doi.org/10.3390%2Fcells11121888
TI - Synthetic Thymidine Analog Labeling without Misconceptions
T2 - Cells
AU - Gruzova, Olesya
AU - Itaman, Sheed
AU - Enikolopov, Grigori
AU - Ivanova, Anna
AU - Ermolaeva, Elizaveta
AU - Astakhova, Olga
AU - Lazutkin, Alexander A.
PY - 2022
DA - 2022/06/10 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 1888
IS - 12
VL - 11
PMID - 35741018
SN - 2073-4409
ER -
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@article{2022_Ivanova,
author = {Olesya Gruzova and Sheed Itaman and Grigori Enikolopov and Anna Ivanova and Elizaveta Ermolaeva and Olga Astakhova and Alexander A. Lazutkin},
title = {Synthetic Thymidine Analog Labeling without Misconceptions},
journal = {Cells},
year = {2022},
volume = {11},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {jun},
url = {https://doi.org/10.3390%2Fcells11121888},
number = {12},
pages = {1888},
doi = {10.3390/cells11121888}
}
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MLA
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Ivanova, Anna, et al. “Synthetic Thymidine Analog Labeling without Misconceptions.” Cells, vol. 11, no. 12, Jun. 2022, p. 1888. https://doi.org/10.3390%2Fcells11121888.
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