Open Access
Open access
MDPI
Cells
,  volume 11 ,  issue 13 ,  pages 1991

Functional Profiling of In Vitro Reactivated Memory B Cells Following Natural SARS-CoV-2 Infection and Gam-COVID-Vac Vaccination

Ekaterina A Astakhova 1, 2
, 
Maria G Byazrova 1, 2, 3
, 
Gaukhar M Yusubalieva 4
, 
Sergey V Kulemzin 5
, 
Natal’ya S. Kruglova 6
, 
Alexey G Prilipov 1, 7
, 
Vladimir P. Baklaushev 4
, 
Andrey A Gorchakov 5
, 
Alexander Taranin 5
, 
A V Filatov 1, 2
1
 
2
 
3
 
4
 
5
 
6
 
7
 
Publication type: Journal Article
Publication date: 2022-06-21
MDPI
scimago Q1
wos Q2
SJR: 1.670
CiteScore: 10.5
Impact factor: 5.2
ISSN: 20734409
General Medicine
Abstract

Both SARS-CoV-2 infection and vaccination have previously been demonstrated to elicit robust, yet somewhat limited immunity against the evolving variants of SARS-CoV-2. Nevertheless, reports performing side-by-side comparison of immune responses following infection vs. vaccination have been relatively scarce. The aim of this study was to compare B-cell response to adenovirus-vectored vaccination in SARS-CoV-2-naive individuals with that observed in the COVID-19 convalescent patients six months after the first encounter with the viral antigens. We set out to use a single analytical platform and performed comprehensive analysis of serum levels of receptor binding domain (RBD)-specific and virus-neutralizing antibodies, frequencies of RBD-binding circulating memory B cells (MBCs), MBC-derived antibody-secreting cells, as well as RBD-specific and virus-neutralizing activity of MBC-derived antibodies after Gam-COVID-Vac (Sputnik V) vaccination and/or natural SARS-CoV-2 infection. Overall, natural immunity was superior to Gam-COVID-Vac vaccination. The levels of neutralizing MBC-derived antibodies in the convalescent patients turned out to be significantly higher than those found following vaccination. Our results suggest that after six months, SARS-CoV-2-specific MBC immunity is more robust in COVID-19 convalescent patients than in Gam-COVID-Vac recipients. Collectively, our data unambiguously indicate that natural immunity outperforms Gam-COVID-Vac-induced immunity six months following recovery/vaccination, which should inform healthcare and vaccination decisions.

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GOST Copy
Astakhova E. A. et al. Functional Profiling of In Vitro Reactivated Memory B Cells Following Natural SARS-CoV-2 Infection and Gam-COVID-Vac Vaccination // Cells. 2022. Vol. 11. No. 13. p. 1991.
GOST all authors (up to 50) Copy
Astakhova E. A., Byazrova M. G., Yusubalieva G. M., Kulemzin S. V., Kruglova N. S., Prilipov A. G., Baklaushev V. P., Gorchakov A. A., Taranin A., Filatov A. V. Functional Profiling of In Vitro Reactivated Memory B Cells Following Natural SARS-CoV-2 Infection and Gam-COVID-Vac Vaccination // Cells. 2022. Vol. 11. No. 13. p. 1991.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.3390/cells11131991
UR - https://doi.org/10.3390/cells11131991
TI - Functional Profiling of In Vitro Reactivated Memory B Cells Following Natural SARS-CoV-2 Infection and Gam-COVID-Vac Vaccination
T2 - Cells
AU - Astakhova, Ekaterina A
AU - Byazrova, Maria G
AU - Yusubalieva, Gaukhar M
AU - Kulemzin, Sergey V
AU - Kruglova, Natal’ya S.
AU - Prilipov, Alexey G
AU - Baklaushev, Vladimir P.
AU - Gorchakov, Andrey A
AU - Taranin, Alexander
AU - Filatov, A V
PY - 2022
DA - 2022/06/21
PB - MDPI
SP - 1991
IS - 13
VL - 11
PMID - 35805076
SN - 2073-4409
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{2022_Astakhova,
author = {Ekaterina A Astakhova and Maria G Byazrova and Gaukhar M Yusubalieva and Sergey V Kulemzin and Natal’ya S. Kruglova and Alexey G Prilipov and Vladimir P. Baklaushev and Andrey A Gorchakov and Alexander Taranin and A V Filatov},
title = {Functional Profiling of In Vitro Reactivated Memory B Cells Following Natural SARS-CoV-2 Infection and Gam-COVID-Vac Vaccination},
journal = {Cells},
year = {2022},
volume = {11},
publisher = {MDPI},
month = {jun},
url = {https://doi.org/10.3390/cells11131991},
number = {13},
pages = {1991},
doi = {10.3390/cells11131991}
}
MLA
Cite this
MLA Copy
Astakhova, Ekaterina A., et al. “Functional Profiling of In Vitro Reactivated Memory B Cells Following Natural SARS-CoV-2 Infection and Gam-COVID-Vac Vaccination.” Cells, vol. 11, no. 13, Jun. 2022, p. 1991. https://doi.org/10.3390/cells11131991.