Open Access

Cells, volume 12, issue 2, pages 290

Chloroquine Enhances Death in Lung Adenocarcinoma A549 Cells Exposed to Cold Atmospheric Plasma Jet

Patrakova Ekaterina ^{1, 2}

Biryukov Mikhail ^{1, 2, 3}

Troitskaya Olga ^{2, 3}

Gugin Pavel ^{3, 4}

Milakhina Elena ^{3, 4, 5}

Semenov Dmitriy ²

Poletaeva Julia ²

Рябчикова Е. И. ^{1, 2}

Novak Diana ^{1, 2, 3}

Kryachkova Nadezhda ¹

Polyakova Alina ¹

Zhilnikova Maria ¹

Zakrevsky Dm. E. ^{3, 4, 5}

Schweigert I.V. ³

Koval Olga ^{1, 2, 3}

Hide authors affiliations Show authors affiliations: 5 affiliations

Department of Natural Sciences, Novosibirsk State University, 630090 Novosibirsk, Russia |

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia |

Khristianovich Institute of Theoretical and Applied Mechanics, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia |

⁴

Rzhanov Institute of Semiconductor Physic, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia |

⁵

Department of Radio Engineering and Electronics, Novosibirsk State Technical University, 630073 Novosibirsk, Russia |

Publication type: Journal Article

Publication date: 2023-01-12

Multidisciplinary Digital Publishing Institute (MDPI)

Journal: Cells

Quartile SCImago

Quartile WOS

Impact factor: 6

ISSN: 20734409

DOI: 10.3390/cells12020290

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General Medicine

Abstract

Cold atmospheric plasma (CAP) is an intensively-studied approach for the treatment of malignant neoplasms. Various active oxygen and nitrogen compounds are believed to be the main cytotoxic effectors on biotargets; however, the comprehensive mechanism of CAP interaction with living cells and tissues remains elusive. In this study, we experimentally determined the optimal discharge regime (or semi-selective regime) for the direct CAP jet treatment of cancer cells, under which lung adenocarcinoma A549, A427 and NCI-H23 cells demonstrated substantial suppression of viability, coupled with a weak viability decrease of healthy lung fibroblasts Wi-38 and MRC-5. The death of CAP-exposed cancer and healthy cells under semi-selective conditions was caspase-dependent. We showed that there was an accumulation of lysosomes in the treated cells. The increased activity of lysosomal protease Cathepsin D, the transcriptional upregulation of autophagy-related MAPLC3B gene in cancer cells and the changes in autophagy-related proteins may have indicated the activation of autophagy. The addition of the autophagy inhibitor chloroquine (CQ) after the CAP jet treatment increased the death of A549 cancer cells in a synergistic manner and showed a low effect on the viability of CAP-treated Wi-38 cells. Downregulation of Drp1 mitochondrial protein and upregulation of PINK1 protein in CAP + CQ treated cells indicated that CQ increased the CAP-dependent destabilization of mitochondria. We concluded that CAP weakly activated pro-survival autophagy in irradiated cells, and CQ promoted CAP-dependent cell death due to the destabilization of autophagosomes formation and mitochondria homeostasis. To summarize, the combination of CAP treatment with CQ could be useful for the development of cold plasma-based antitumor approaches for clinical application.

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	1 2
Applied Sciences (Switzerland)	Applied Sciences (Switzerland), 2, 33.33% Applied Sciences (Switzerland) 2 publications, 33.33%
Catalysts	Catalysts, 1, 16.67% Catalysts 1 publication, 16.67%
Biomolecules	Biomolecules, 1, 16.67% Biomolecules 1 publication, 16.67%
International Journal of Molecular Sciences	International Journal of Molecular Sciences, 1, 16.67% International Journal of Molecular Sciences 1 publication, 16.67%
Journal Physics D: Applied Physics	Journal Physics D: Applied Physics, 1, 16.67% Journal Physics D: Applied Physics 1 publication, 16.67%
	1 2

Citations by publishers

	1 2 3 4 5
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 5, 83.33% Multidisciplinary Digital Publishing Institute (MDPI) 5 publications, 83.33%
IOP Publishing	IOP Publishing, 1, 16.67% IOP Publishing 1 publication, 16.67%
	1 2 3 4 5

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Patrakova E. et al. Chloroquine Enhances Death in Lung Adenocarcinoma A549 Cells Exposed to Cold Atmospheric Plasma Jet // Cells. 2023. Vol. 12. No. 2. p. 290.

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Patrakova E., Biryukov M., Troitskaya O., Gugin P., Milakhina E., Semenov D., Poletaeva J., Рябчикова Е. И., Novak D., Kryachkova N., Polyakova A., Zhilnikova M., Zakrevsky D. E., Schweigert I., Koval O. Chloroquine Enhances Death in Lung Adenocarcinoma A549 Cells Exposed to Cold Atmospheric Plasma Jet // Cells. 2023. Vol. 12. No. 2. p. 290.

RIS |

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RIS Copy

TY - JOUR

DO - 10.3390/cells12020290

UR - https://doi.org/10.3390%2Fcells12020290

TI - Chloroquine Enhances Death in Lung Adenocarcinoma A549 Cells Exposed to Cold Atmospheric Plasma Jet

T2 - Cells

AU - Patrakova, Ekaterina

AU - Biryukov, Mikhail

AU - Troitskaya, Olga

AU - Gugin, Pavel

AU - Milakhina, Elena

AU - Semenov, Dmitriy

AU - Poletaeva, Julia

AU - Рябчикова, Е. И.

AU - Novak, Diana

AU - Kryachkova, Nadezhda

AU - Polyakova, Alina

AU - Zhilnikova, Maria

AU - Zakrevsky, Dm. E.

AU - Schweigert, I.V.

AU - Koval, Olga

PY - 2023

DA - 2023/01/12 00:00:00

PB - Multidisciplinary Digital Publishing Institute (MDPI)

SP - 290

IS - 2

VL - 12

SN - 2073-4409

ER -

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@article{2023_Patrakova,

author = {Ekaterina Patrakova and Mikhail Biryukov and Olga Troitskaya and Pavel Gugin and Elena Milakhina and Dmitriy Semenov and Julia Poletaeva and Е. И. Рябчикова and Diana Novak and Nadezhda Kryachkova and Alina Polyakova and Maria Zhilnikova and Dm. E. Zakrevsky and I.V. Schweigert and Olga Koval},

title = {Chloroquine Enhances Death in Lung Adenocarcinoma A549 Cells Exposed to Cold Atmospheric Plasma Jet},

journal = {Cells},

year = {2023},

volume = {12},

publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},

month = {jan},

url = {https://doi.org/10.3390%2Fcells12020290},

number = {2},

pages = {290},

doi = {10.3390/cells12020290}

}

MLA

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MLA Copy

Patrakova, Ekaterina, et al. “Chloroquine Enhances Death in Lung Adenocarcinoma A549 Cells Exposed to Cold Atmospheric Plasma Jet.” Cells, vol. 12, no. 2, Jan. 2023, p. 290. https://doi.org/10.3390%2Fcells12020290.

Found error?

Publisher

Multidisciplinary Digital Publishing Institute (MDPI)

Journal

Cells

Quartile SCImago

Quartile WOS

Impact factor

ISSN

20734409 (Print)

Labs

Laboratory of Genomic Editing

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