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International Journal of Molecular Sciences, volume 22, issue 24, pages 13663

Effects of Microvesicles Derived from NK Cells Stimulated with IL-1β on the Phenotype and Functional Activity of Endothelial Cells

Markova Kseniia ¹

Mikhailova Valentina ¹

Milyutina Yulia ¹

Korenevsky Andrey ¹

Sirotskaya Anastasia ¹

Rodygina Veronika ¹

Tyshchuk Elizaveta ¹

Grebenkina P V ¹

Simbirtsev Andrey ²

Selkov Sergey ¹

Sokolov Dmitry I. ¹

Hide authors affiliations Show authors affiliations: 2 affiliations

Department of Immunology and Intercellular Interactions, Federal State Budgetary Scientific Institution, Research Institute of Obstetrics, Gynecology, and Reproductology Named after D.O. Ott, 199034 St. Petersburg, Russia |

State Research Institute of Highly Pure Biopreparations, 197110 St. Petersburg, Russia |

Publication type: Journal Article

Publication date: 2021-12-20

Multidisciplinary Digital Publishing Institute (MDPI)

Journal: International Journal of Molecular Sciences

Quartile SCImago

Quartile WOS

Impact factor: 5.6

ISSN: 16616596, 14220067

DOI: 10.3390/ijms222413663

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PubMed ID: 34948459

Catalysis

Organic Chemistry

Inorganic Chemistry

Physical and Theoretical Chemistry

Computer Science Applications

Spectroscopy

Molecular Biology

General Medicine

Abstract

Microvesicles (MVs) are plasma extracellular vesicles ranging from 100 (150) to 1000 nm in diameter. These are generally produced by different cells through their vital activity and are a source of various protein and non-protein molecules. It is assumed that MVs can mediate intercellular communication and modulate cell functions. The interaction between natural killer cells (NK cells) and endothelial cells underlies multiple pathological conditions. The ability of MVs derived from NK cells to influence the functional state of endothelial cells in inflammatory conditions has yet to be studied well. In this regard, we aimed to study the effects of MVs derived from NK cells of the NK-92 cell line stimulated with IL-1β on the phenotype, caspase activity, proliferation and migration of endothelial cells of the EA.hy926 cell line. Endothelial cells were cultured with MVs derived from cells of the NK-92 cell line after their stimulation with IL-1β. Using flow cytometry, we evaluated changes in the expression of endothelial cell surface molecules and endothelial cell death. We evaluated the effect of MVs derived from stimulated NK cells on the proliferative and migratory activity of endothelial cells, as well as the activation of caspase-3 and caspase-9 therein. It was established that the incubation of endothelial cells with MVs derived from cells of the NK-92 cell line stimulated with IL-1β and with MVs derived from unstimulated NK cells, leads to the decrease in the proliferative activity of endothelial cells, appearance of the pan leukocyte marker CD45 on them, caspase-3 activation and partial endothelial cell death, and reduced CD105 expression. However, compared with MVs derived from unstimulated NK cells, a more pronounced effect of MVs derived from cells of the NK-92 cell line stimulated with IL-1β was found in relation to the decrease in the endothelial cell migratory activity and the intensity of the CD54 molecule expression on them. The functional activity of MVs is therefore mediated by the conditions they are produced under, as well as their internal contents.

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International Journal of Molecular Sciences	International Journal of Molecular Sciences, 1, 20% International Journal of Molecular Sciences 1 publication, 20%
Membranes	Membranes, 1, 20% Membranes 1 publication, 20%
Biomolecules	Biomolecules, 1, 20% Biomolecules 1 publication, 20%
Frontiers in Bioengineering and Biotechnology	Frontiers in Bioengineering and Biotechnology, 1, 20% Frontiers in Bioengineering and Biotechnology 1 publication, 20%
Frontiers in Immunology	Frontiers in Immunology, 1, 20% Frontiers in Immunology 1 publication, 20%
	1

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Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 3, 60% Multidisciplinary Digital Publishing Institute (MDPI) 3 publications, 60%
Frontiers Media S.A.	Frontiers Media S.A., 2, 40% Frontiers Media S.A. 2 publications, 40%
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Markova K. et al. Effects of Microvesicles Derived from NK Cells Stimulated with IL-1β on the Phenotype and Functional Activity of Endothelial Cells // International Journal of Molecular Sciences. 2021. Vol. 22. No. 24. p. 13663.

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Markova K., Mikhailova V., Milyutina Y., Korenevsky A., Sirotskaya A., Rodygina V., Tyshchuk E., Grebenkina P. V., Simbirtsev A., Selkov S., Sokolov D. I. Effects of Microvesicles Derived from NK Cells Stimulated with IL-1β on the Phenotype and Functional Activity of Endothelial Cells // International Journal of Molecular Sciences. 2021. Vol. 22. No. 24. p. 13663.

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TY - JOUR

DO - 10.3390/ijms222413663

UR - https://doi.org/10.3390%2Fijms222413663

TI - Effects of Microvesicles Derived from NK Cells Stimulated with IL-1β on the Phenotype and Functional Activity of Endothelial Cells

T2 - International Journal of Molecular Sciences

AU - Markova, Kseniia

AU - Milyutina, Yulia

AU - Sirotskaya, Anastasia

AU - Rodygina, Veronika

AU - Simbirtsev, Andrey

AU - Selkov, Sergey

AU - Mikhailova, Valentina

AU - Korenevsky, Andrey

AU - Tyshchuk, Elizaveta

AU - Grebenkina, P V

AU - Sokolov, Dmitry I.

PY - 2021

DA - 2021/12/20 00:00:00

PB - Multidisciplinary Digital Publishing Institute (MDPI)

SP - 13663

IS - 24

VL - 22

PMID - 34948459

SN - 1661-6596

SN - 1422-0067

ER -

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@article{2021_Markova,

author = {Kseniia Markova and Yulia Milyutina and Anastasia Sirotskaya and Veronika Rodygina and Andrey Simbirtsev and Sergey Selkov and Valentina Mikhailova and Andrey Korenevsky and Elizaveta Tyshchuk and P V Grebenkina and Dmitry I. Sokolov},

title = {Effects of Microvesicles Derived from NK Cells Stimulated with IL-1β on the Phenotype and Functional Activity of Endothelial Cells},

journal = {International Journal of Molecular Sciences},

year = {2021},

volume = {22},

publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},

month = {dec},

url = {https://doi.org/10.3390%2Fijms222413663},

number = {24},

pages = {13663},

doi = {10.3390/ijms222413663}

}

MLA

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MLA Copy

Markova, Kseniia, et al. “Effects of Microvesicles Derived from NK Cells Stimulated with IL-1β on the Phenotype and Functional Activity of Endothelial Cells.” International Journal of Molecular Sciences, vol. 22, no. 24, Dec. 2021, p. 13663. https://doi.org/10.3390%2Fijms222413663.

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Publisher

Multidisciplinary Digital Publishing Institute (MDPI)

Journal

International Journal of Molecular Sciences

Quartile SCImago

Quartile WOS

Impact factor

5.6

ISSN

16616596 (Print)
14220067 (Electronic)

Labs

Department of Immunology and Intercellular Interactions

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