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International Journal of Molecular Sciences, volume 24, issue 6, pages 5104

Dissection of Antibody Responses of Gam-COVID-Vac-Vaccinated Subjects Suggests Involvement of Epitopes Outside RBD in SARS-CoV-2 Neutralization

Byazrova Maria ^{1, 2, 3}

Gattinger Pia ⁴

Astakhova Ekaterina ^{1, 2}

HOFER Gerhard ⁵

Khaitov Musa ^{1, 6}

Filatov A V ^{1, 2}

Valenta Rudolf ^{1, 4, 7, 8}

Hide authors affiliations Show authors affiliations: 8 affiliations

National Research Center—Institute of Immunology FMBA of Russia, 115478 Moscow, Russia |

Department of Immunology, Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia |

Department of Immunology, Peoples’ Friendship University of Russia (RUDN University) of Ministry of Science and Higher Education of the Russian Federation, 117198 Moscow, Russia |

⁴

Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, Austria |

⁵

Department of Materials and Environmental Chemistry, University of Stockholm, 106 91 Stockholm, Sweden |

⁶

Department of Immunology, Pirogov Russian National Research Medical University, 117997 Moscow, Russia |

⁷

Department of Clinical Immunology and Allergy, Sechenov First Moscow State Medical University, 119435 Moscow, Russia |

⁸

Karl Landsteiner University of Health Sciences, 3500 Krems, Austria |

Publication type: Journal Article

Publication date: 2023-03-07

Multidisciplinary Digital Publishing Institute (MDPI)

Journal: International Journal of Molecular Sciences

Quartile SCImago

Quartile WOS

Impact factor: 5.6

ISSN: 16616596, 14220067

DOI: 10.3390/ijms24065104

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PubMed ID: 36982183

Catalysis

Organic Chemistry

Inorganic Chemistry

Physical and Theoretical Chemistry

Computer Science Applications

Spectroscopy

Molecular Biology

General Medicine

Abstract

Millions of people have been vaccinated with Gam-COVID-Vac but fine specificities of induced antibodies have not been fully studied. Plasma from 12 naïve and 10 coronavirus disease 2019 (COVID-19) convalescent subjects was obtained before and after two immunizations with Gam-COVID-Vac. Antibody reactivity in the plasma samples (n = 44) was studied on a panel of micro-arrayed recombinant folded and unfolded severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteins and 46 peptides spanning the spike protein (S) and by immunoglobulin G (IgG) subclass enzyme-linked immunosorbent assay (ELISA). The ability of Gam-COVID-Vac-induced antibodies to inhibit binding of the receptor-binding domain (RBD) to its receptor angiotensin converting enzyme 2 (ACE2) was investigated in a molecular interaction assay (MIA). The virus-neutralizing capacity of antibodies was studied by the pseudo-typed virus neutralization test (pVNT) for Wuhan-Hu-1 and Omicron. We found that Gam-COVID-Vac vaccination induced significant increases of IgG1 but not of other IgG subclasses against folded S, spike protein subunit 1 (S1), spike protein subunit 2 (S2), and RBD in a comparable manner in naïve and convalescent subjects. Virus neutralization was highly correlated with vaccination-induced antibodies specific for folded RBD and a novel peptide (i.e., peptide 12). Peptide 12 was located close to RBD in the N-terminal part of S1 and may potentially be involved in the transition of the pre- to post-fusion conformation of the spike protein. In summary, Gam-COVID-Vac vaccination induced S-specific IgG1 antibodies in naive and convalescent subjects in a comparable manner. Besides the antibodies specific for RBD, the antibodies induced against a peptide close to the N-terminus of RBD were also associated with virus-neutralization.

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International Journal of Molecular Sciences	International Journal of Molecular Sciences, 1, 50% International Journal of Molecular Sciences 1 publication, 50%
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Byazrova M. et al. Dissection of Antibody Responses of Gam-COVID-Vac-Vaccinated Subjects Suggests Involvement of Epitopes Outside RBD in SARS-CoV-2 Neutralization // International Journal of Molecular Sciences. 2023. Vol. 24. No. 6. p. 5104.

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Byazrova M., Gattinger P., Astakhova E., HOFER G., Khaitov M., Filatov A. V., Valenta R. Dissection of Antibody Responses of Gam-COVID-Vac-Vaccinated Subjects Suggests Involvement of Epitopes Outside RBD in SARS-CoV-2 Neutralization // International Journal of Molecular Sciences. 2023. Vol. 24. No. 6. p. 5104.

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TY - JOUR

DO - 10.3390/ijms24065104

UR - https://doi.org/10.3390%2Fijms24065104

TI - Dissection of Antibody Responses of Gam-COVID-Vac-Vaccinated Subjects Suggests Involvement of Epitopes Outside RBD in SARS-CoV-2 Neutralization

T2 - International Journal of Molecular Sciences

AU - Byazrova, Maria

AU - Gattinger, Pia

AU - Astakhova, Ekaterina

AU - HOFER, Gerhard

AU - Khaitov, Musa

AU - Filatov, A V

AU - Valenta, Rudolf

PY - 2023

DA - 2023/03/07 00:00:00

PB - Multidisciplinary Digital Publishing Institute (MDPI)

SP - 5104

IS - 6

VL - 24

PMID - 36982183

SN - 1661-6596

SN - 1422-0067

ER -

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@article{2023_Byazrova,

author = {Maria Byazrova and Pia Gattinger and Ekaterina Astakhova and Gerhard HOFER and Musa Khaitov and A V Filatov and Rudolf Valenta},

title = {Dissection of Antibody Responses of Gam-COVID-Vac-Vaccinated Subjects Suggests Involvement of Epitopes Outside RBD in SARS-CoV-2 Neutralization},

journal = {International Journal of Molecular Sciences},

year = {2023},

volume = {24},

publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},

month = {mar},

url = {https://doi.org/10.3390%2Fijms24065104},

number = {6},

pages = {5104},

doi = {10.3390/ijms24065104}

}

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Byazrova, Maria, et al. “Dissection of Antibody Responses of Gam-COVID-Vac-Vaccinated Subjects Suggests Involvement of Epitopes Outside RBD in SARS-CoV-2 Neutralization.” International Journal of Molecular Sciences, vol. 24, no. 6, Mar. 2023, p. 5104. https://doi.org/10.3390%2Fijms24065104.

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Publisher

Multidisciplinary Digital Publishing Institute (MDPI)

Journal

International Journal of Molecular Sciences

Quartile SCImago

Quartile WOS

Impact factor

5.6

ISSN

16616596 (Print)
14220067 (Electronic)

Labs

Laboratory of Immunochemistry

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