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International Journal of Molecular Sciences, volume 24, issue 23, pages 16723

Enhancing the Antimicrobial Properties of Peptides through Cell-Penetrating Peptide Conjugation: A Comprehensive Assessment

Kravchenko Sergey V. ¹

Domnin Pavel A ^{2, 3}

Grishin Sergei Y. ^{1, 4}

VERSHININ Nikita A. ¹

Gurina Elena V. ¹

Zakharova Anastasiia A ⁵

Azev Viatcheslav ⁶

Mustaeva Leila G. ⁶

Gorbunova Elena Y ⁶

Kobyakova M I ^{7, 8}

Surin Alexey K. ^{4, 6, 9}

Fadeev Roman S. ⁷

Ostroumova Olga S. ⁵

Ermolaeva Svetlana A. ³

Галзитская О. В. ^{4, 7}

Hide authors affiliations Show authors affiliations: 9 affiliations

Institute of Environmental and Agricultural Biology (X-BIO), Tyumen State University, 625003 Tyumen, Russia |

Biology Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia |

Gamaleya Research Centre of Epidemiology and Microbiology, 123098 Moscow, Russia |

⁴

Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Russia |

⁵

Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg, Russia |

⁶

The Branch of the Institute of Bioorganic Chemistry, Russian Academy of Sciences, 142290 Pushchino, Russia |

⁷

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Russia |

⁸

Research Institute of Clinical and Experimental Lymphology—Branch of the Institute of Cytology and Genetics Siberian Branch of Russian Academy of Sciences, 630060 Novosibirsk, Russia

Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences

Research Institute of Clinical and Experimental Lymphology ICG of the Siberian Branch of the Russian Academy of Sciences

⁹

State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Russia |

Publication type: Journal Article

Publication date: 2023-11-24

Multidisciplinary Digital Publishing Institute (MDPI)

Journal: International Journal of Molecular Sciences

Quartile SCImago

Quartile WOS

Impact factor: 5.6

ISSN: 16616596, 14220067

DOI: 10.3390/ijms242316723

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Catalysis

Organic Chemistry

Inorganic Chemistry

Physical and Theoretical Chemistry

Computer Science Applications

Spectroscopy

Molecular Biology

General Medicine

Abstract

Combining antimicrobial peptides (AMPs) with cell-penetrating peptides (CPPs) has shown promise in boosting antimicrobial potency, especially against Gram-negative bacteria. We examined the CPP-AMP interaction with distinct bacterial types based on cell wall differences. Our investigation focused on AMPs incorporating penetratin CPP and dihybrid peptides containing both cell-penetrating TAT protein fragments from the human immunodeficiency virus and Antennapedia peptide (Antp). Assessment of the peptides TAT-AMP, AMP-Antp, and TAT-AMP-Antp revealed their potential against Gram-positive strains (Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus (MRSA), and Bacillus cereus). Peptides TAT-AMP and AMP-Antp using an amyloidogenic AMP from S1 ribosomal protein Thermus thermophilus, at concentrations ranging from 3 to 12 μM, exhibited enhanced antimicrobial activity against B. cereus. TAT-AMP and TAT-AMP-Antp, using an amyloidogenic AMP from the S1 ribosomal protein Pseudomonas aeruginosa, at a concentration of 12 µM, demonstrated potent antimicrobial activity against S. aureus and MRSA. Notably, the TAT-AMP, at a concentration of 12 µM, effectively inhibited Escherichia coli (E. coli) growth and displayed antimicrobial effects similar to gentamicin after 15 h of incubation. Peptide characteristics determined antimicrobial activity against diverse strains. The study highlights the intricate relationship between peptide properties and antimicrobial potential. Mechanisms of AMP action are closely tied to bacterial cell wall attributes. Peptides with the TAT fragment exhibited enhanced antimicrobial activity against S. aureus, MRSA, and P. aeruginosa. Peptides containing only the Antp fragment displayed lower activity. None of the investigated peptides demonstrated cytotoxic or cytostatic effects on either BT-474 cells or human skin fibroblasts. In conclusion, CPP-AMPs offer promise against various bacterial strains, offering insights for targeted antimicrobial development.

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Kravchenko S. V. et al. Enhancing the Antimicrobial Properties of Peptides through Cell-Penetrating Peptide Conjugation: A Comprehensive Assessment // International Journal of Molecular Sciences. 2023. Vol. 24. No. 23. p. 16723.

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Kravchenko S. V., Domnin P. A., Grishin S. Y., VERSHININ N. A., Gurina E. V., Zakharova A. A., Azev V., Mustaeva L. G., Gorbunova E. Y., Kobyakova M. I., Surin A. K., Fadeev R. S., Ostroumova O. S., Ermolaeva S. A., Галзитская О. В. Enhancing the Antimicrobial Properties of Peptides through Cell-Penetrating Peptide Conjugation: A Comprehensive Assessment // International Journal of Molecular Sciences. 2023. Vol. 24. No. 23. p. 16723.

RIS |

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TY - JOUR

DO - 10.3390/ijms242316723

UR - https://doi.org/10.3390%2Fijms242316723

TI - Enhancing the Antimicrobial Properties of Peptides through Cell-Penetrating Peptide Conjugation: A Comprehensive Assessment

T2 - International Journal of Molecular Sciences

AU - Kravchenko, Sergey V.

AU - Domnin, Pavel A

AU - Grishin, Sergei Y.

AU - VERSHININ, Nikita A.

AU - Gurina, Elena V.

AU - Zakharova, Anastasiia A

AU - Azev, Viatcheslav

AU - Mustaeva, Leila G.

AU - Gorbunova, Elena Y

AU - Kobyakova, M I

AU - Surin, Alexey K.

AU - Fadeev, Roman S.

AU - Ostroumova, Olga S.

AU - Ermolaeva, Svetlana A.

AU - Галзитская, О. В.

PY - 2023

DA - 2023/11/24 00:00:00

PB - Multidisciplinary Digital Publishing Institute (MDPI)

SP - 16723

IS - 23

VL - 24

SN - 1661-6596

SN - 1422-0067

ER -

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@article{2023_Kravchenko,

author = {Sergey V. Kravchenko and Pavel A Domnin and Sergei Y. Grishin and Nikita A. VERSHININ and Elena V. Gurina and Anastasiia A Zakharova and Viatcheslav Azev and Leila G. Mustaeva and Elena Y Gorbunova and M I Kobyakova and Alexey K. Surin and Roman S. Fadeev and Olga S. Ostroumova and Svetlana A. Ermolaeva and О. В. Галзитская},

title = {Enhancing the Antimicrobial Properties of Peptides through Cell-Penetrating Peptide Conjugation: A Comprehensive Assessment},

journal = {International Journal of Molecular Sciences},

year = {2023},

volume = {24},

publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},

month = {nov},

url = {https://doi.org/10.3390%2Fijms242316723},

number = {23},

pages = {16723},

doi = {10.3390/ijms242316723}

}

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Kravchenko, Sergey V., et al. “Enhancing the Antimicrobial Properties of Peptides through Cell-Penetrating Peptide Conjugation: A Comprehensive Assessment.” International Journal of Molecular Sciences, vol. 24, no. 23, Nov. 2023, p. 16723. https://doi.org/10.3390%2Fijms242316723.

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Publisher

Multidisciplinary Digital Publishing Institute (MDPI)

Journal

International Journal of Molecular Sciences

Quartile SCImago

Quartile WOS

Impact factor

5.6

ISSN

16616596 (Print)
14220067 (Electronic)

Labs

Antimicrobial Resistance Laboratory

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