Open Access
Plants, volume 11, issue 1, pages 51
Hairy CRISPR: Genome Editing in Plants Using Hairy Root Transformation
Publication type: Journal Article
Publication date: 2021-12-24
Plant Science
Ecology, Evolution, Behavior and Systematics
Ecology
Abstract
CRISPR/Cas-mediated genome editing is a powerful tool of plant functional genomics. Hairy root transformation is a rapid and convenient approach for obtaining transgenic roots. When combined, these techniques represent a fast and effective means of studying gene function. In this review, we outline the current state of the art reached by the combination of these approaches over seven years. Additionally, we discuss the origins of different Agrobacterium rhizogenes strains that are widely used for hairy root transformation; the components of CRISPR/Cas vectors, such as the promoters that drive Cas or gRNA expression, the types of Cas nuclease, and selectable and screenable markers; and the application of CRISPR/Cas genome editing in hairy roots. The modification of the already known vector pKSE401 with the addition of the rice translational enhancer OsMac3 and the gene encoding the fluorescent protein DsRed1 is also described.
Citations by journals
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International Journal of Molecular Sciences
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International Journal of Molecular Sciences
5 publications, 19.23%
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3 Biotech
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1 publication, 3.85%
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PLoS ONE
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PLoS ONE
1 publication, 3.85%
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1 publication, 3.85%
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1 publication, 3.85%
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1 publication, 3.85%
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1 publication, 3.85%
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1 publication, 3.85%
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1 publication, 3.85%
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1 publication, 3.85%
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Methods in Molecular Biology
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1 publication, 3.85%
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Citations by publishers
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
8 publications, 30.77%
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Springer Nature
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Springer Nature
6 publications, 23.08%
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Frontiers Media S.A.
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Frontiers Media S.A.
3 publications, 11.54%
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Public Library of Science (PLoS)
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Public Library of Science (PLoS)
1 publication, 3.85%
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Elsevier
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Elsevier
1 publication, 3.85%
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Wiley
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Wiley
1 publication, 3.85%
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American Society for Microbiology
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American Society for Microbiology
1 publication, 3.85%
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Eco-Vector LLC
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Eco-Vector LLC
1 publication, 3.85%
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Oxford University Press
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Oxford University Press
1 publication, 3.85%
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Cold Spring Harbor Laboratory
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Cold Spring Harbor Laboratory
1 publication, 3.85%
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Kiryushkin A. S. et al. Hairy CRISPR: Genome Editing in Plants Using Hairy Root Transformation // Plants. 2021. Vol. 11. No. 1. p. 51.
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Kiryushkin A. S., Ilina E. L., Guseva E. D., Pawlowski K., Demchenko K. Hairy CRISPR: Genome Editing in Plants Using Hairy Root Transformation // Plants. 2021. Vol. 11. No. 1. p. 51.
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TY - JOUR
DO - 10.3390/plants11010051
UR - https://doi.org/10.3390%2Fplants11010051
TI - Hairy CRISPR: Genome Editing in Plants Using Hairy Root Transformation
T2 - Plants
AU - Kiryushkin, Alexey S
AU - Ilina, Elena L
AU - Guseva, Elizaveta D
AU - Pawlowski, Katharina
AU - Demchenko, Kirill
PY - 2021
DA - 2021/12/24 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 51
IS - 1
VL - 11
SN - 2223-7747
ER -
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@article{2021_Kiryushkin,
author = {Alexey S Kiryushkin and Elena L Ilina and Elizaveta D Guseva and Katharina Pawlowski and Kirill Demchenko},
title = {Hairy CRISPR: Genome Editing in Plants Using Hairy Root Transformation},
journal = {Plants},
year = {2021},
volume = {11},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {dec},
url = {https://doi.org/10.3390%2Fplants11010051},
number = {1},
pages = {51},
doi = {10.3390/plants11010051}
}
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MLA
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Kiryushkin, Alexey S., et al. “Hairy CRISPR: Genome Editing in Plants Using Hairy Root Transformation.” Plants, vol. 11, no. 1, Dec. 2021, p. 51. https://doi.org/10.3390%2Fplants11010051.