Open Access
Sensors, volume 22, issue 16, pages 6049
Electroactive Biofilms of Activated Sludge Microorganisms on a Nanostructured Surface as the Basis for a Highly Sensitive Biochemical Oxygen Demand Biosensor
Kurbanalieva Saniyat
1
,
Kharkova Anna
1
,
Perchikov Roman
1
,
Kamanina Olga
1
,
Melnikov Pavel V
2
,
Popova Nadezhda
3
,
Мачулин А. В.
4
,
Tarasov Sergey E
4
,
Publication type: Journal Article
Publication date: 2022-08-12
PubMed ID:
36015810
Biochemistry
Analytical Chemistry
Atomic and Molecular Physics, and Optics
Electrical and Electronic Engineering
Instrumentation
Abstract
The possibility of the developing a biochemical oxygen demand (BOD) biosensor based on electroactive biofilms of activated sludge grown on the surface of a graphite-paste electrode modified with carbon nanotubes was studied. A complex of microscopic methods controlled biofilm formation: optical microscopy with phase contrast, scanning electron microscopy, and laser confocal microscopy. The features of charge transfer in the obtained electroactive biofilms were studied using the methods of cyclic voltammetry and electrochemical impedance spectroscopy. The rate constant of the interaction of microorganisms with the extracellular electron carrier (0.79 ± 0.03 dm3(g s)−1) and the heterogeneous rate constant of electron transfer (0.34 ± 0.02 cm s−1) were determined using the cyclic voltammetry method. These results revealed that the modification of the carbon nanotubes’ (CNT) electrode surface makes it possible to create electroactive biofilms. An analysis of the metrological and analytical characteristics of the created biosensors showed that the lower limit of the biosensor based on an electroactive biofilm of activated sludge is 0.41 mgO2/dm3, which makes it possible to analyze almost any water sample. Analysis of 12 surface water samples showed a high correlation (R2 = 0.99) with the results of the standard method for determining biochemical oxygen demand.
Citations by journals
|
1
2
3
|
|
|
Polymers
|
Polymers
3 publications, 33.33%
|
|
Biosensors
|
Biosensors
2 publications, 22.22%
|
|
Sensors
|
Sensors
2 publications, 22.22%
|
|
3 Biotech
|
3 Biotech
1 publication, 11.11%
|
|
ACS applied materials & interfaces
|
ACS applied materials & interfaces
1 publication, 11.11%
|
|
1
2
3
|
Citations by publishers
|
1
2
3
4
5
6
7
|
|
|
Multidisciplinary Digital Publishing Institute (MDPI)
|
Multidisciplinary Digital Publishing Institute (MDPI)
7 publications, 77.78%
|
|
Springer Nature
|
Springer Nature
1 publication, 11.11%
|
|
American Chemical Society (ACS)
|
American Chemical Society (ACS)
1 publication, 11.11%
|
|
1
2
3
4
5
6
7
|
- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2022,2023],"ids":[0,0],"codes":[0,0],"imageUrls":["",""],"datasets":[{"label":"Citations number","data":[4,5],"backgroundColor":["#3B82F6","#3B82F6"],"percentage":["44.44","55.56"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Polymers","Biosensors","Sensors","3 Biotech","ACS applied materials & interfaces"],"ids":[11204,5233,18435,20049,1458],"codes":[0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp"],"datasets":[{"label":"","data":[3,2,2,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[33.33,22.22,22.22,11.11,11.11],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Multidisciplinary Digital Publishing Institute (MDPI)","Springer Nature","American Chemical Society (ACS)"],"ids":[202,8,40],"codes":[0,0,0],"imageUrls":["\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/iLiQsFqFaSEx6chlGQ5fbAwF6VYU3WWa08hkss0g_medium.webp"],"datasets":[{"label":"","data":[7,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6"],"percentage":[77.78,11.11,11.11],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}
Metrics
Cite this
GOST |
RIS |
BibTex |
MLA
Cite this
GOST
Copy
Kurbanalieva S. et al. Electroactive Biofilms of Activated Sludge Microorganisms on a Nanostructured Surface as the Basis for a Highly Sensitive Biochemical Oxygen Demand Biosensor // Sensors. 2022. Vol. 22. No. 16. p. 6049.
GOST all authors (up to 50)
Copy
Kurbanalieva S., Arlyapov V. A., Kharkova A., Perchikov R., Kamanina O., Melnikov P. V., Popova N., Мачулин А. В., Tarasov S. E., Saverina E., Vereshchagin A. N., Reshetilov A. N. Electroactive Biofilms of Activated Sludge Microorganisms on a Nanostructured Surface as the Basis for a Highly Sensitive Biochemical Oxygen Demand Biosensor // Sensors. 2022. Vol. 22. No. 16. p. 6049.
Cite this
RIS
Copy
TY - JOUR
DO - 10.3390/s22166049
UR - https://doi.org/10.3390%2Fs22166049
TI - Electroactive Biofilms of Activated Sludge Microorganisms on a Nanostructured Surface as the Basis for a Highly Sensitive Biochemical Oxygen Demand Biosensor
T2 - Sensors
AU - Kurbanalieva, Saniyat
AU - Kharkova, Anna
AU - Perchikov, Roman
AU - Kamanina, Olga
AU - Saverina, Evgeniya
AU - Arlyapov, Vyacheslav A
AU - Melnikov, Pavel V
AU - Popova, Nadezhda
AU - Мачулин, А. В.
AU - Tarasov, Sergey E
AU - Vereshchagin, Anatoly N.
AU - Reshetilov, A. N.
PY - 2022
DA - 2022/08/12 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 6049
IS - 16
VL - 22
PMID - 36015810
SN - 1424-3210
SN - 1424-8220
ER -
Cite this
BibTex
Copy
@article{2022_Kurbanalieva,
author = {Saniyat Kurbanalieva and Anna Kharkova and Roman Perchikov and Olga Kamanina and Evgeniya Saverina and Vyacheslav A Arlyapov and Pavel V Melnikov and Nadezhda Popova and А. В. Мачулин and Sergey E Tarasov and Anatoly N. Vereshchagin and A. N. Reshetilov},
title = {Electroactive Biofilms of Activated Sludge Microorganisms on a Nanostructured Surface as the Basis for a Highly Sensitive Biochemical Oxygen Demand Biosensor},
journal = {Sensors},
year = {2022},
volume = {22},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {aug},
url = {https://doi.org/10.3390%2Fs22166049},
number = {16},
pages = {6049},
doi = {10.3390/s22166049}
}
Cite this
MLA
Copy
Kurbanalieva, Saniyat, et al. “Electroactive Biofilms of Activated Sludge Microorganisms on a Nanostructured Surface as the Basis for a Highly Sensitive Biochemical Oxygen Demand Biosensor.” Sensors, vol. 22, no. 16, Aug. 2022, p. 6049. https://doi.org/10.3390%2Fs22166049.