Open Access
Antibiotics, volume 11, issue 9, pages 1198
Mechanism-Based Approach to New Antibiotic Producers Screening among Actinomycetes in the Course of the Citizen Science Project
Volynkina Inna A
1, 2
,
Zakalyukina Yuliya V
3, 4
,
Alferova Vera A
5, 6
,
Belik Albina R
3
,
Yagoda Daria K
7
,
Buyuklyan Yuliya A
3
,
Udalov Andrei V
3
,
Golovin Evgenii V
3
,
Kryakvin Maxim A
7
,
Lukianov Dmitrii A
1, 2
,
Biryukov Mikhail V
2, 3, 8
,
Sergiev Petr V.
1, 2
,
Dontsova Olga A.
1, 2, 6
,
Osterman Ilya A
1, 2, 3
1
Publication type: Journal Article
Publication date: 2022-09-05
PubMed ID:
36139977
Biochemistry
Microbiology (medical)
Microbiology
Infectious Diseases
Pharmacology (medical)
General Pharmacology, Toxicology and Pharmaceutics
Abstract
Since the discovery of streptomycin, actinomycetes have been a useful source for new antibiotics, but there have been diminishing rates of new finds since the 1960s. The decreasing probability of identifying new active agents led to reduced interest in soil bacteria as a source for new antibiotics. At the same time, actinomycetes remain a promising reservoir for new active molecules. In this work, we present several reporter plasmids encoding visible fluorescent protein genes. These plasmids provide primary information about the action mechanism of antimicrobial agents at an early stage of screening. The reporters and the pipeline described have been optimized and designed to employ citizen scientists without specialized skills or equipment with the aim of essentially crowdsourcing the search for new antibiotic producers in the vast natural reservoir of soil bacteria. The combination of mechanism-based approaches and citizen science has proved its effectiveness in practice, revealing a significant increase in the screening rate. As a proof of concept, two new strains, Streptomyces sp. KB-1 and BV113, were found to produce the antibiotics pikromycin and chartreusin, respectively, demonstrating the efficiency of the pipeline.
Citations by journals
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1
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Russian Chemical Bulletin
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Russian Chemical Bulletin
1 publication, 50%
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Russian Entomological Journal
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Russian Entomological Journal
1 publication, 50%
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1
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Citations by publishers
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1
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Springer Nature
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Springer Nature
1 publication, 50%
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KMK Scientific Press
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KMK Scientific Press
1 publication, 50%
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1
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- We do not take into account publications that without a DOI.
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- Statistics recalculated weekly.
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Volynkina I. A. et al. Mechanism-Based Approach to New Antibiotic Producers Screening among Actinomycetes in the Course of the Citizen Science Project // Antibiotics. 2022. Vol. 11. No. 9. p. 1198.
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Volynkina I. A., Zakalyukina Y. V., Alferova V. A., Belik A. R., Yagoda D. K., Nikandrova A. A., Buyuklyan Y. A., Udalov A. V., Golovin E. V., Kryakvin M. A., Lukianov D. A., Biryukov M. V., Sergiev P. V., Dontsova O. A., Osterman I. A. Mechanism-Based Approach to New Antibiotic Producers Screening among Actinomycetes in the Course of the Citizen Science Project // Antibiotics. 2022. Vol. 11. No. 9. p. 1198.
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TY - JOUR
DO - 10.3390/antibiotics11091198
UR - https://doi.org/10.3390%2Fantibiotics11091198
TI - Mechanism-Based Approach to New Antibiotic Producers Screening among Actinomycetes in the Course of the Citizen Science Project
T2 - Antibiotics
AU - Belik, Albina R
AU - Yagoda, Daria K
AU - Buyuklyan, Yuliya A
AU - Udalov, Andrei V
AU - Golovin, Evgenii V
AU - Dontsova, Olga A.
AU - Kryakvin, Maxim A
AU - Volynkina, Inna A
AU - Zakalyukina, Yuliya V
AU - Alferova, Vera A
AU - Nikandrova, Arina A
AU - Lukianov, Dmitrii A
AU - Biryukov, Mikhail V
AU - Sergiev, Petr V.
AU - Osterman, Ilya A
PY - 2022
DA - 2022/09/05 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 1198
IS - 9
VL - 11
PMID - 36139977
SN - 2079-6382
ER -
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@article{2022_Volynkina,
author = {Albina R Belik and Daria K Yagoda and Yuliya A Buyuklyan and Andrei V Udalov and Evgenii V Golovin and Olga A. Dontsova and Maxim A Kryakvin and Inna A Volynkina and Yuliya V Zakalyukina and Vera A Alferova and Arina A Nikandrova and Dmitrii A Lukianov and Mikhail V Biryukov and Petr V. Sergiev and Ilya A Osterman},
title = {Mechanism-Based Approach to New Antibiotic Producers Screening among Actinomycetes in the Course of the Citizen Science Project},
journal = {Antibiotics},
year = {2022},
volume = {11},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {sep},
url = {https://doi.org/10.3390%2Fantibiotics11091198},
number = {9},
pages = {1198},
doi = {10.3390/antibiotics11091198}
}
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MLA
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Volynkina, Inna A., et al. “Mechanism-Based Approach to New Antibiotic Producers Screening among Actinomycetes in the Course of the Citizen Science Project.” Antibiotics, vol. 11, no. 9, Sep. 2022, p. 1198. https://doi.org/10.3390%2Fantibiotics11091198.