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Antioxidants, том 10, издание 12, номер публикации: 1985

Oxidative Damage Induced by Phototoxic Pheophorbide a 17-Diethylene Glycol Ester Encapsulated in PLGA Nanoparticles

Nikolskaya Elena 1, 2
Beganovskaya Veronika 2, 3
Sokol Maria 1, 2
Obydennyi Sergey 4, 5
Mollaev Murad D. 5
Тип документаJournal Article
Дата публикации2021-12-13
ИздательMultidisciplinary Digital Publishing Institute (MDPI)
Название журналаAntioxidants
Квартиль по SCImagoQ1
Квартиль по Web of ScienceQ1
Импакт-фактор 20217.68
Molecular Biology
Cell Biology
Clinical Biochemistry
Краткое описание

Pheophorbide a 17-diethylene glycol ester (XL-8), is a promising high-active derivative of known photosensitizer chlorin e6 used in photodynamic therapy. However, high lipophilicity and poor tumor accumulation limit XL-8 therapeutic application. We developed a novel XL-8 loaded with poly(D,L-lactide-co-glycolide) nanoparticles using the single emulsion-solvent evaporation method. The nanoparticles possessed high XL-8 loading content (4.6%) and encapsulation efficiency (87.7%) and a small size (182 ± 19 nm), and negative surface charge (−22.2 ± 3.8 mV) contributed to a specific intracellular accumulation. Sustained biphasic XL-8 release from nanoparticles enhanced the photosensitizer photostability upon irradiation that could potentially reduce the quantity of the drug applied. Additionally, the encapsulation of XL-8 in the polymer matrix preserved phototoxic activity of the payload. The nanoparticles displayed enhanced cellular internalization. Flow cytometry and confocal laser-scanning microscopy studies revealed rapid XL-8 loaded nanoparticles distribution throughout the cell and initiation of DNA damage, glutathione depletion, and lipid peroxidation via reactive oxygen species formation. The novel nanoformulated XL-8 simultaneously revealed a significant phototoxicity accompanied with enhanced photostability, in contrast with traditional photosensitizers, and demonstrated a great potential for further in vivo studies.

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1. Mollaeva M.R. и др. Oxidative Damage Induced by Phototoxic Pheophorbide a 17-Diethylene Glycol Ester Encapsulated in PLGA Nanoparticles // Antioxidants. 2021. Т. 10. № 12. С. 1985.


DO - 10.3390/antiox10121985

UR -

TI - Oxidative Damage Induced by Phototoxic Pheophorbide a 17-Diethylene Glycol Ester Encapsulated in PLGA Nanoparticles

T2 - Antioxidants

AU - Mollaeva, Mariia R.

AU - Nikolskaya, Elena

AU - Beganovskaya, Veronika

AU - Sokol, Maria

AU - Chirkina, Margarita

AU - Obydennyi, Sergey

AU - Belykh, Dmitry

AU - Startseva, Olga

AU - Mollaev, Murad D.

AU - Yabbarov, Nikita

PY - 2021

DA - 2021/12/13


SP - 1985

IS - 12

VL - 10

SN - 2076-3921

ER -

BibTex |


doi = {10.3390/antiox10121985},

url = {},

year = 2021,

month = {dec},

publisher = {{MDPI} {AG}},

volume = {10},

number = {12},

pages = {1985},

author = {Mariia R. Mollaeva and Elena Nikolskaya and Veronika Beganovskaya and Maria Sokol and Margarita Chirkina and Sergey Obydennyi and Dmitry Belykh and Olga Startseva and Murad D. Mollaev and Nikita Yabbarov},

title = {Oxidative Damage Induced by Phototoxic Pheophorbide a 17-Diethylene Glycol Ester Encapsulated in {PLGA} Nanoparticles},

journal = {Antioxidants}


Mollaeva, Mariia R. et al. “Oxidative Damage Induced by Phototoxic Pheophorbide a 17-Diethylene Glycol Ester Encapsulated in PLGA Nanoparticles.” Antioxidants 10.12 (2021): 1985. Crossref. Web.