Open Access
Biology, volume 10, issue 2, pages 1-21
Osteoclasts differentiation from murine RAW 264.7 cells stimulated by RANKL: Timing and behavior
Publication type: Journal Article
Publication date: 2021-02-04
PubMed ID:
33557437
General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences
General Immunology and Microbiology
Abstract
The development of multi-nucleated cells is critical for osteoclasts (OCs) maturation and function. Our objective was to extend knowledge on osteoclastogenesis, focusing on pre-OC fusion timing and behavior. RAW 264.7 cells, which is a murine monocyte-macrophage cell line, provide a valuable and widely used tool for in vitro studies on osteoclastogenesis mechanisms. Cells were treated with the receptor activator of nuclear factor κ-B ligand (RANKL) for 1–4 days and effects on cell morphology, cytoskeletal organization, protein distribution, and OC-specific gene expression examined by TEM, immunofluorescence, and qPCR. Multinucleated cells began to appear at two days of Receptor Activator of Nuclear factor κ-B Ligand (RANKL) stimulation, increasing in number and size in the following days, associated with morphological and cytoskeletal organization changes. Interesting cellular extensions were observed in three days within cells labeled with wheat germ agglutinin (WGA)-Fluorescein isothiocyanate (FITC). The membrane, cytoplasmic, or nuclear distribution of RANK, TRAF6, p-p38, pERK1/2, and NFATc1, respectively, was related to OCs maturation timing. The gene expression for transcription factors regulating osteoclastogenesis (NFATc1, c-fos, RelA, MITF), molecules involved in RANKL-signaling transduction (TRAF6), cytoskeleton regulation (RhoA), fusion (DC-STAMP), migration (MMP9), and OC-specific enzymes (TRAP, CtsK), showed different trends related to OC differentiation timing. Our findings provide an integrated view on the morphological and molecular changes occurring during RANKL stimulation of RAW 264.7 cells, which are important to better understand the OCs’ maturation processes.
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Lampiasi N. et al. Osteoclasts differentiation from murine RAW 264.7 cells stimulated by RANKL: Timing and behavior // Biology. 2021. Vol. 10. No. 2. pp. 1-21.
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Lampiasi N., Russo R., Kireev I., Strelkova O., Zhironkina O., Zito F. Osteoclasts differentiation from murine RAW 264.7 cells stimulated by RANKL: Timing and behavior // Biology. 2021. Vol. 10. No. 2. pp. 1-21.
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TY - JOUR
DO - 10.3390/biology10020117
UR - https://doi.org/10.3390%2Fbiology10020117
TI - Osteoclasts differentiation from murine RAW 264.7 cells stimulated by RANKL: Timing and behavior
T2 - Biology
AU - Kireev, Igor
AU - Strelkova, Olga
AU - Zhironkina, Oxana
AU - Lampiasi, Nadia
AU - Russo, Roberta
AU - Zito, Francesca
PY - 2021
DA - 2021/02/04 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 1-21
IS - 2
VL - 10
PMID - 33557437
SN - 2079-7737
ER -
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@article{2021_Lampiasi,
author = {Igor Kireev and Olga Strelkova and Oxana Zhironkina and Nadia Lampiasi and Roberta Russo and Francesca Zito},
title = {Osteoclasts differentiation from murine RAW 264.7 cells stimulated by RANKL: Timing and behavior},
journal = {Biology},
year = {2021},
volume = {10},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {feb},
url = {https://doi.org/10.3390%2Fbiology10020117},
number = {2},
pages = {1--21},
doi = {10.3390/biology10020117}
}
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Lampiasi, Nadia, et al. “Osteoclasts differentiation from murine RAW 264.7 cells stimulated by RANKL: Timing and behavior.” Biology, vol. 10, no. 2, Feb. 2021, pp. 1-21. https://doi.org/10.3390%2Fbiology10020117.