Open Access
Open access
International Journal of Molecular Sciences, volume 21, issue 19, pages 7362

Gene Editing by Extracellular Vesicles

Brezgin Sergey 1, 2
Smirnov Valery 2, 3
Volchkova Elena 3
Lukashev Alexander 3
Publication typeJournal Article
Publication date2020-10-05
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor5.6
ISSN16616596, 14220067
PubMed ID:  33028045
Catalysis
Organic Chemistry
Inorganic Chemistry
Physical and Theoretical Chemistry
Computer Science Applications
Spectroscopy
Molecular Biology
General Medicine
Abstract

CRISPR/Cas technologies have advanced dramatically in recent years. Many different systems with new properties have been characterized and a plethora of hybrid CRISPR/Cas systems able to modify the epigenome, regulate transcription, and correct mutations in DNA and RNA have been devised. However, practical application of CRISPR/Cas systems is severely limited by the lack of effective delivery tools. In this review, recent advances in developing vehicles for the delivery of CRISPR/Cas in the form of ribonucleoprotein complexes are outlined. Most importantly, we emphasize the use of extracellular vesicles (EVs) for CRISPR/Cas delivery and describe their unique properties: biocompatibility, safety, capacity for rational design, and ability to cross biological barriers. Available molecular tools that enable loading of desired protein and/or RNA cargo into the vesicles in a controllable manner and shape the surface of EVs for targeted delivery into specific tissues (e.g., using targeting ligands, peptides, or nanobodies) are discussed. Opportunities for both endogenous (intracellular production of CRISPR/Cas) and exogenous (post-production) loading of EVs are presented.

Citations by journals

1
2
3
Pharmaceutics
Pharmaceutics, 3, 10%
Pharmaceutics
3 publications, 10%
Molecular Biology
Molecular Biology, 2, 6.67%
Molecular Biology
2 publications, 6.67%
Journal of Nanobiotechnology
Journal of Nanobiotechnology, 2, 6.67%
Journal of Nanobiotechnology
2 publications, 6.67%
Molecular Therapy - Nucleic Acids
Molecular Therapy - Nucleic Acids, 2, 6.67%
Molecular Therapy - Nucleic Acids
2 publications, 6.67%
Viruses
Viruses, 1, 3.33%
Viruses
1 publication, 3.33%
Nucleic Acid Therapeutics
Nucleic Acid Therapeutics, 1, 3.33%
Nucleic Acid Therapeutics
1 publication, 3.33%
Human Gene Therapy
Human Gene Therapy, 1, 3.33%
Human Gene Therapy
1 publication, 3.33%
International Journal of Molecular Sciences
International Journal of Molecular Sciences, 1, 3.33%
International Journal of Molecular Sciences
1 publication, 3.33%
Biomedicines
Biomedicines, 1, 3.33%
Biomedicines
1 publication, 3.33%
Pharmaceuticals
Pharmaceuticals, 1, 3.33%
Pharmaceuticals
1 publication, 3.33%
Cells
Cells, 1, 3.33%
Cells
1 publication, 3.33%
Molecular Pharmaceutics
Molecular Pharmaceutics, 1, 3.33%
Molecular Pharmaceutics
1 publication, 3.33%
Frontiers in Genome Editing
Frontiers in Genome Editing, 1, 3.33%
Frontiers in Genome Editing
1 publication, 3.33%
Stem Cell Research and Therapy
Stem Cell Research and Therapy, 1, 3.33%
Stem Cell Research and Therapy
1 publication, 3.33%
Cell Biology International
Cell Biology International, 1, 3.33%
Cell Biology International
1 publication, 3.33%
Molecular Therapy
Molecular Therapy, 1, 3.33%
Molecular Therapy
1 publication, 3.33%
Advanced Drug Delivery Reviews
Advanced Drug Delivery Reviews, 1, 3.33%
Advanced Drug Delivery Reviews
1 publication, 3.33%
Biotechnology Advances
Biotechnology Advances, 1, 3.33%
Biotechnology Advances
1 publication, 3.33%
Reviews in Medical Virology
Reviews in Medical Virology, 1, 3.33%
Reviews in Medical Virology
1 publication, 3.33%
Seminars in Cancer Biology
Seminars in Cancer Biology, 1, 3.33%
Seminars in Cancer Biology
1 publication, 3.33%
Biomedicine and Pharmacotherapy
Biomedicine and Pharmacotherapy, 1, 3.33%
Biomedicine and Pharmacotherapy
1 publication, 3.33%
Advances in Experimental Medicine and Biology
Advances in Experimental Medicine and Biology, 1, 3.33%
Advances in Experimental Medicine and Biology
1 publication, 3.33%
1
2
3

Citations by publishers

1
2
3
4
5
6
7
8
Multidisciplinary Digital Publishing Institute (MDPI)
Multidisciplinary Digital Publishing Institute (MDPI), 8, 26.67%
Multidisciplinary Digital Publishing Institute (MDPI)
8 publications, 26.67%
Elsevier
Elsevier, 7, 23.33%
Elsevier
7 publications, 23.33%
Springer Nature
Springer Nature, 4, 13.33%
Springer Nature
4 publications, 13.33%
Mary Ann Liebert
Mary Ann Liebert, 2, 6.67%
Mary Ann Liebert
2 publications, 6.67%
Pleiades Publishing
Pleiades Publishing, 2, 6.67%
Pleiades Publishing
2 publications, 6.67%
Wiley
Wiley, 2, 6.67%
Wiley
2 publications, 6.67%
American Chemical Society (ACS)
American Chemical Society (ACS), 1, 3.33%
American Chemical Society (ACS)
1 publication, 3.33%
Frontiers Media S.A.
Frontiers Media S.A., 1, 3.33%
Frontiers Media S.A.
1 publication, 3.33%
1
2
3
4
5
6
7
8
  • We do not take into account publications that without a DOI.
  • Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
  • Statistics recalculated weekly.
Metrics
Share
Cite this
GOST |
Cite this
GOST Copy
Kostyushev D. et al. Gene Editing by Extracellular Vesicles // International Journal of Molecular Sciences. 2020. Vol. 21. No. 19. p. 7362.
GOST all authors (up to 50) Copy
Kostyushev D., Kostyusheva A., Brezgin S., Smirnov V., Volchkova E., Lukashev A., Chulanov V. Gene Editing by Extracellular Vesicles // International Journal of Molecular Sciences. 2020. Vol. 21. No. 19. p. 7362.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.3390/ijms21197362
UR - https://doi.org/10.3390%2Fijms21197362
TI - Gene Editing by Extracellular Vesicles
T2 - International Journal of Molecular Sciences
AU - Kostyusheva, Anastasiya
AU - Brezgin, Sergey
AU - Volchkova, Elena
AU - Lukashev, Alexander
AU - Smirnov, Valery
AU - Kostyushev, Dmitry
AU - Chulanov, Vladimir
PY - 2020
DA - 2020/10/05 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 7362
IS - 19
VL - 21
PMID - 33028045
SN - 1661-6596
SN - 1422-0067
ER -
BibTex |
Cite this
BibTex Copy
@article{2020_Kostyushev
author = {Anastasiya Kostyusheva and Sergey Brezgin and Elena Volchkova and Alexander Lukashev and Valery Smirnov and Dmitry Kostyushev and Vladimir Chulanov},
title = {Gene Editing by Extracellular Vesicles},
journal = {International Journal of Molecular Sciences},
year = {2020},
volume = {21},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {oct},
url = {https://doi.org/10.3390%2Fijms21197362},
number = {19},
pages = {7362},
doi = {10.3390/ijms21197362}
}
MLA
Cite this
MLA Copy
Kostyushev, Dmitry, et al. “Gene Editing by Extracellular Vesicles.” International Journal of Molecular Sciences, vol. 21, no. 19, Oct. 2020, p. 7362. https://doi.org/10.3390%2Fijms21197362.
Found error?