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International Journal of Molecular Sciences, том 22, издание 19, номер публикации: 10221

Comparative analysis of genome editors efficiency on a model of mice zygotes microinjection

Averina Olga A. 1, 2
Permyakov Oleg A. 1
Grigorieva Olga O. 1
Mazur Alexander M. 3
Prokhortchouk Egor B. 3
Dontsova Olga A. 4, 5, 6
Sergiev Petr V. 1, 4, 5
Тип документаJournal Article
Дата публикации2021-09-23
ИздательMultidisciplinary Digital Publishing Institute (MDPI)
Название журналаInternational Journal of Molecular Sciences
Квартиль по SCImagoQ1
Квартиль по Web of ScienceQ1
Импакт-фактор 20216.21
ISSN16616596, 14220067
Catalysis
Organic Chemistry
Inorganic Chemistry
Physical and Theoretical Chemistry
Computer Science Applications
Spectroscopy
Molecular Biology
General Medicine
Краткое описание

Genome editing is an indispensable tool for functional genomics. The caveat of the genome-editing pipeline is a prevalence of error-prone non-homologous end joining over homologous recombination, while only the latter is suitable to introduce particularly desired genetic variants. To overcome this problem, a toolbox of genome engineering was appended by a variety of improved instruments. In this work, we compared the efficiency of a number of recently suggested improved systems for genome editing applied to the same genome regions on a murine zygote model via microinjection. As a result, we observed that homologous recombination utilizing an ssDNA template following sgRNA directed Cas9 cleavage is still the method of choice for the creation of animals with precise genome alterations.

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1. Averina O.A. и др. Comparative Analysis of Genome Editors Efficiency on a Model of Mice Zygotes Microinjection // International Journal of Molecular Sciences. 2021. Т. 22. № 19. С. 10221.
RIS |
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TY - JOUR

DO - 10.3390/ijms221910221

UR - http://dx.doi.org/10.3390/ijms221910221

TI - Comparative Analysis of Genome Editors Efficiency on a Model of Mice Zygotes Microinjection

T2 - International Journal of Molecular Sciences

AU - Averina, Olga A.

AU - Permyakov, Oleg A.

AU - Grigorieva, Olga O.

AU - Starshin, Alexey S.

AU - Mazur, Alexander M.

AU - Prokhortchouk, Egor B.

AU - Dontsova, Olga A.

AU - Sergiev, Petr V.

PY - 2021

DA - 2021/09/23

PB - MDPI AG

SP - 10221

IS - 19

VL - 22

SN - 1422-0067

ER -

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@article{2021,

doi = {10.3390/ijms221910221},

url = {https://doi.org/10.3390%2Fijms221910221},

year = 2021,

month = {sep},

publisher = {{MDPI} {AG}},

volume = {22},

number = {19},

pages = {10221},

author = {Olga A. Averina and Oleg A. Permyakov and Olga O. Grigorieva and Alexey S. Starshin and Alexander M. Mazur and Egor B. Prokhortchouk and Olga A. Dontsova and Petr V. Sergiev},

title = {Comparative Analysis of Genome Editors Efficiency on a Model of Mice Zygotes Microinjection}

}

MLA
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Averina, Olga A. et al. “Comparative Analysis of Genome Editors Efficiency on a Model of Mice Zygotes Microinjection.” International Journal of Molecular Sciences 22.19 (2021): 10221. Crossref. Web.