Open Access
Comparative analysis of genome editors efficiency on a model of mice zygotes microinjection
Olga A Averina
1, 2
,
Oleg A Permyakov
1
,
Olga O Grigorieva
1
,
Alexander M Mazur
3
,
Egor B Prokhortchouk
3
,
Olga A. Dontsova
4, 5, 6
,
Petr V. Sergiev
1, 4, 5
Publication type: Journal Article
Publication date: 2021-09-23
scimago Q1
wos Q1
SJR: 1.273
CiteScore: 9.0
Impact factor: 4.9
ISSN: 16616596, 14220067
PubMed ID:
34638562
Catalysis
Organic Chemistry
Inorganic Chemistry
Physical and Theoretical Chemistry
Computer Science Applications
Spectroscopy
Molecular Biology
General Medicine
Abstract
Genome editing is an indispensable tool for functional genomics. The caveat of the genome-editing pipeline is a prevalence of error-prone non-homologous end joining over homologous recombination, while only the latter is suitable to introduce particularly desired genetic variants. To overcome this problem, a toolbox of genome engineering was appended by a variety of improved instruments. In this work, we compared the efficiency of a number of recently suggested improved systems for genome editing applied to the same genome regions on a murine zygote model via microinjection. As a result, we observed that homologous recombination utilizing an ssDNA template following sgRNA directed Cas9 cleavage is still the method of choice for the creation of animals with precise genome alterations.
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Averina O. A. et al. Comparative analysis of genome editors efficiency on a model of mice zygotes microinjection // International Journal of Molecular Sciences. 2021. Vol. 22. No. 19. p. 10221.
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Averina O. A., Permyakov O. A., Grigorieva O. O., Starshin A. S., Mazur A. M., Prokhortchouk E. B., Dontsova O. A., Sergiev P. V. Comparative analysis of genome editors efficiency on a model of mice zygotes microinjection // International Journal of Molecular Sciences. 2021. Vol. 22. No. 19. p. 10221.
Cite this
RIS
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TY - JOUR
DO - 10.3390/ijms221910221
UR - https://www.mdpi.com/1422-0067/22/19/10221
TI - Comparative analysis of genome editors efficiency on a model of mice zygotes microinjection
T2 - International Journal of Molecular Sciences
AU - Averina, Olga A
AU - Permyakov, Oleg A
AU - Grigorieva, Olga O
AU - Starshin, Alexey S
AU - Mazur, Alexander M
AU - Prokhortchouk, Egor B
AU - Dontsova, Olga A.
AU - Sergiev, Petr V.
PY - 2021
DA - 2021/09/23
PB - MDPI
SP - 10221
IS - 19
VL - 22
PMID - 34638562
SN - 1661-6596
SN - 1422-0067
ER -
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BibTex (up to 50 authors)
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@article{2021_Averina,
author = {Olga A Averina and Oleg A Permyakov and Olga O Grigorieva and Alexey S Starshin and Alexander M Mazur and Egor B Prokhortchouk and Olga A. Dontsova and Petr V. Sergiev},
title = {Comparative analysis of genome editors efficiency on a model of mice zygotes microinjection},
journal = {International Journal of Molecular Sciences},
year = {2021},
volume = {22},
publisher = {MDPI},
month = {sep},
url = {https://www.mdpi.com/1422-0067/22/19/10221},
number = {19},
pages = {10221},
doi = {10.3390/ijms221910221}
}
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MLA
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Averina, Olga A., et al. “Comparative analysis of genome editors efficiency on a model of mice zygotes microinjection.” International Journal of Molecular Sciences, vol. 22, no. 19, Sep. 2021, p. 10221. https://www.mdpi.com/1422-0067/22/19/10221.
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