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International Journal of Molecular Sciences, том 23, издание 12, номер публикации: 6453

RtcB2‐PrfH Operon Protects E. coli ATCC25992 Strain from Colicin E3 Toxin

Тип документаJournal Article
Дата публикации2022-06-09
ИздательMultidisciplinary Digital Publishing Institute (MDPI)
Название журналаInternational Journal of Molecular Sciences
Квартиль по SCImagoQ1
Квартиль по Web of ScienceQ1
Импакт-фактор 20216.21
ISSN16616596, 14220067
Catalysis
Organic Chemistry
Inorganic Chemistry
Physical and Theoretical Chemistry
Computer Science Applications
Spectroscopy
Molecular Biology
General Medicine
Краткое описание
In the bid to survive and thrive in an environmental setting, bacterial species constantly interact and compete for resources and space in the microbial ecosystem. Thus, they have adapted to use various antibiotics and toxins to fight their rivals. Simultaneously, they have evolved an ability to withstand weapons that are directed against them. Several bacteria harbor colicinogenic plasmids which encode toxins that impair the translational apparatus. One of them, colicin E3 ribotoxin, mediates cleavage of the 16S rRNA in the decoding center of the ribosome. In order to thrive upon deployment of such ribotoxins, competing bacteria may have evolved counter-conflict mechanisms to prevent their demise. A recent study demonstrated the role of PrfH and the RtcB2 module in rescuing a damaged ribosome and the subsequent re-ligation of the cleaved 16S rRNA by colicin E3 in vitro. The rtcB2-prfH genes coexist as gene neighbors in an operon that is sporadically spread among different bacteria. In the current study, we report that the RtcB2-PrfH module confers resistance to colicin E3 toxicity in E. coli ATCC25922 cells in vivo. We demonstrated that the viability of E. coli ATCC25922 strain that is devoid of rtcB2 and prfH genes is impaired upon action of colicin E3, in contrast to the parental strain which has intact rtcB2 and prfH genes. Complementation of the rtcB2 and prfH gene knockout with a high copy number-plasmid (encoding either rtcB2 alone or both rtcB2-prfH operon) restored resistance to colicin E3. These results highlight a counter-conflict system that may have evolved to thwart colicin E3 activity.
Пристатейные ссылки: 51
Цитируется в публикациях: 1
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The RNA ligase RtcB reverses MazF-induced ribosome heterogeneity in Escherichia coli
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RNA damage in biological conflicts and the diversity of responding RNA repair systems
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Mechanisms of ribosome rescue in bacteria
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Accumulation of single-stranded DNA in Escherichia coli carrying the colicin plasmid pColE3-CA38.
Morales M., Attai H., Troy K., Bermudes D.
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Colicin import into E. coli cells: a model system for insights into the import mechanisms of bacteriocins.
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Winther K.S., Brodersen D.E., Brown A.K., Gerdes K.
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Rewriting the rules for end joining via enzymatic splicing of DNA 3′-PO4 and 5′-OH ends
Das U., Chakravarty A.K., Remus B.S., Shuman S.
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1. Maviza T. P. и др. RtcB2-PrfH Operon Protects E. coli ATCC25922 Strain from Colicin E3 Toxin // International Journal of Molecular Sciences. 2022. Т. 23. № 12. С. 6453.
RIS |
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TY - JOUR

DO - 10.3390/ijms23126453

UR - http://dx.doi.org/10.3390/ijms23126453

TI - RtcB2-PrfH Operon Protects E. coli ATCC25922 Strain from Colicin E3 Toxin

T2 - International Journal of Molecular Sciences

AU - Maviza, Tinashe P.

AU - Zarechenskaia, Anastasiia S.

AU - Burmistrova, Nadezhda R.

AU - Tchoub, Andrey S.

AU - Dontsova, Olga A.

AU - Sergiev, Petr V.

AU - Osterman, Ilya A.

PY - 2022

DA - 2022/06/09

PB - MDPI AG

SP - 6453

IS - 12

VL - 23

SN - 1422-0067

ER -

BibTex |
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@article{Maviza_2022,

doi = {10.3390/ijms23126453},

url = {https://doi.org/10.3390%2Fijms23126453},

year = 2022,

month = {jun},

publisher = {{MDPI} {AG}},

volume = {23},

number = {12},

pages = {6453},

author = {Tinashe P. Maviza and Anastasiia S. Zarechenskaia and Nadezhda R. Burmistrova and Andrey S. Tchoub and Olga A. Dontsova and Petr V. Sergiev and Ilya A. Osterman},

title = {{RtcB}2-{PrfH} Operon Protects E. coli {ATCC}25922 Strain from Colicin E3 Toxin},

journal = {International Journal of Molecular Sciences}

}

MLA
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Maviza, Tinashe P., et al. “RtcB2-PrfH Operon Protects E. Coli ATCC25922 Strain from Colicin E3 Toxin.” International Journal of Molecular Sciences, vol. 23, no. 12, June 2022, p. 6453. Crossref, https://doi.org/10.3390/ijms23126453.