Open Access
International Journal of Molecular Sciences, volume 23, issue 12, pages 6453
RtcB2‐PrfH Operon Protects E. coli ATCC25992 Strain from Colicin E3 Toxin
Maviza Tinashe P
1
,
Zarechenskaia Anastasiia S
1, 2
,
Burmistrova Nadezhda R
2
,
Tchoub Andrey S
2
,
Dontsova Olga A.
1, 2, 3
,
Sergiev Petr V.
1, 2
,
Osterman Ilya A
1, 2, 4
Publication type: Journal Article
Publication date: 2022-06-09
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 5.6
ISSN: 16616596, 14220067
PubMed ID:
35742896
Catalysis
Organic Chemistry
Inorganic Chemistry
Physical and Theoretical Chemistry
Computer Science Applications
Spectroscopy
Molecular Biology
General Medicine
Abstract
In the bid to survive and thrive in an environmental setting, bacterial species constantly interact and compete for resources and space in the microbial ecosystem. Thus, they have adapted to use various antibiotics and toxins to fight their rivals. Simultaneously, they have evolved an ability to withstand weapons that are directed against them. Several bacteria harbor colicinogenic plasmids which encode toxins that impair the translational apparatus. One of them, colicin E3 ribotoxin, mediates cleavage of the 16S rRNA in the decoding center of the ribosome. In order to thrive upon deployment of such ribotoxins, competing bacteria may have evolved counter-conflict mechanisms to prevent their demise. A recent study demonstrated the role of PrfH and the RtcB2 module in rescuing a damaged ribosome and the subsequent re-ligation of the cleaved 16S rRNA by colicin E3 in vitro. The rtcB2-prfH genes coexist as gene neighbors in an operon that is sporadically spread among different bacteria. In the current study, we report that the RtcB2-PrfH module confers resistance to colicin E3 toxicity in E. coli ATCC25922 cells in vivo. We demonstrated that the viability of E. coli ATCC25922 strain that is devoid of rtcB2 and prfH genes is impaired upon action of colicin E3, in contrast to the parental strain which has intact rtcB2 and prfH genes. Complementation of the rtcB2 and prfH gene knockout with a high copy number-plasmid (encoding either rtcB2 alone or both rtcB2-prfH operon) restored resistance to colicin E3. These results highlight a counter-conflict system that may have evolved to thwart colicin E3 activity.
Citations by journals
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1
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Nucleic Acids Research
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Nucleic Acids Research
1 publication, 33.33%
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Nature Reviews Microbiology
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Nature Reviews Microbiology
1 publication, 33.33%
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1
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Citations by publishers
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1
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Oxford University Press
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Oxford University Press
1 publication, 33.33%
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Springer Nature
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Springer Nature
1 publication, 33.33%
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1
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- We do not take into account publications that without a DOI.
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- Statistics recalculated weekly.
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Maviza T. P. et al. RtcB2‐PrfH Operon Protects E. coli ATCC25992 Strain from Colicin E3 Toxin // International Journal of Molecular Sciences. 2022. Vol. 23. No. 12. p. 6453.
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Maviza T. P., Zarechenskaia A. S., Burmistrova N. R., Tchoub A. S., Dontsova O. A., Sergiev P. V., Osterman I. A. RtcB2‐PrfH Operon Protects E. coli ATCC25992 Strain from Colicin E3 Toxin // International Journal of Molecular Sciences. 2022. Vol. 23. No. 12. p. 6453.
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TY - JOUR
DO - 10.3390/ijms23126453
UR - https://doi.org/10.3390%2Fijms23126453
TI - RtcB2‐PrfH Operon Protects E. coli ATCC25992 Strain from Colicin E3 Toxin
T2 - International Journal of Molecular Sciences
AU - Zarechenskaia, Anastasiia S
AU - Burmistrova, Nadezhda R
AU - Tchoub, Andrey S
AU - Dontsova, Olga A.
AU - Sergiev, Petr V.
AU - Maviza, Tinashe P
AU - Osterman, Ilya A
PY - 2022
DA - 2022/06/09 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 6453
IS - 12
VL - 23
PMID - 35742896
SN - 1661-6596
SN - 1422-0067
ER -
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@article{2022_Maviza,
author = {Anastasiia S Zarechenskaia and Nadezhda R Burmistrova and Andrey S Tchoub and Olga A. Dontsova and Petr V. Sergiev and Tinashe P Maviza and Ilya A Osterman},
title = {RtcB2‐PrfH Operon Protects E. coli ATCC25992 Strain from Colicin E3 Toxin},
journal = {International Journal of Molecular Sciences},
year = {2022},
volume = {23},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {jun},
url = {https://doi.org/10.3390%2Fijms23126453},
number = {12},
pages = {6453},
doi = {10.3390/ijms23126453}
}
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MLA
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Maviza, Tinashe P., et al. “RtcB2‐PrfH Operon Protects E. coli ATCC25992 Strain from Colicin E3 Toxin.” International Journal of Molecular Sciences, vol. 23, no. 12, Jun. 2022, p. 6453. https://doi.org/10.3390%2Fijms23126453.