Open Access
Microorganisms, volume 9, issue 12, pages 2554
Steroid Metabolism in Thermophilic Actinobacterium Saccharopolyspora hirsuta VKM Ac-666T
Lobastova Tatyana
1
,
Fokina Victoria
1
,
Shutov Andrey
1
,
Bragin Eugeny
1
,
Kazantsev Alexey
2
,
Donova Marina
1
Publication type: Journal Article
Publication date: 2021-12-10
PubMed ID:
34946155
Microbiology (medical)
Microbiology
Virology
Abstract
The application of thermophilic microorganisms opens new prospects in steroid biotechnology, but little is known to date on steroid catabolism by thermophilic strains. The thermophilic strain Saccharopolyspora hirsuta VKM Ac-666T has been shown to convert various steroids and to fully degrade cholesterol. Cholest-4-en-3-one, cholesta-1,4-dien-3-one, 26-hydroxycholest-4-en-3-one, 3-oxo-cholest-4-en-26-oic acid, 3-oxo-cholesta-1,4-dien-26-oic acid, 26-hydroxycholesterol, 3β-hydroxy-cholest-5-en-26-oic acid were identified as intermediates in cholesterol oxidation. The structures were confirmed by 1H and 13C-NMR analyses. Aliphatic side chain hydroxylation at C26 and the A-ring modification at C3, which are putatively catalyzed by cytochrome P450 monooxygenase CYP125 and cholesterol oxidase, respectively, occur simultaneously in the strain and are followed by cascade reactions of aliphatic sidechain degradation and steroid core destruction via the known 9(10)-seco-pathway. The genes putatively related to the sterol and bile acid degradation pathways form three major clusters in the S. hirsuta genome. The sets of the genes include the orthologs of those involved in steroid catabolism in Mycobacterium tuberculosis H37Rv and Rhodococcus jostii RHA1 and related actinobacteria. Bioinformatics analysis of 52 publicly available genomes of thermophilic bacteria revealed only seven candidate strains that possess the key genes related to the 9(10)-seco pathway of steroid degradation, thus demonstrating that the ability to degrade steroids is not widespread among thermophilic bacteria.
Citations by journals
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1
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Bioresources and Bioprocessing
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Bioresources and Bioprocessing
1 publication, 25%
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Journal of Steroid Biochemistry and Molecular Biology
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Journal of Steroid Biochemistry and Molecular Biology
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Microbiology
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Microbiology
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Methods in Molecular Biology
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Methods in Molecular Biology
1 publication, 25%
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1
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Citations by publishers
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1
2
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Springer Nature
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Springer Nature
2 publications, 50%
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Elsevier
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Elsevier
1 publication, 25%
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Pleiades Publishing
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Pleiades Publishing
1 publication, 25%
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1
2
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- We do not take into account publications that without a DOI.
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Lobastova T. et al. Steroid Metabolism in Thermophilic Actinobacterium Saccharopolyspora hirsuta VKM Ac-666T // Microorganisms. 2021. Vol. 9. No. 12. p. 2554.
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Lobastova T., Fokina V., Tarlachkov S., Shutov A., Bragin E., Kazantsev A., Donova M. Steroid Metabolism in Thermophilic Actinobacterium Saccharopolyspora hirsuta VKM Ac-666T // Microorganisms. 2021. Vol. 9. No. 12. p. 2554.
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TY - JOUR
DO - 10.3390/microorganisms9122554
UR - https://doi.org/10.3390%2Fmicroorganisms9122554
TI - Steroid Metabolism in Thermophilic Actinobacterium Saccharopolyspora hirsuta VKM Ac-666T
T2 - Microorganisms
AU - Lobastova, Tatyana
AU - Fokina, Victoria
AU - Tarlachkov, Sergey
AU - Shutov, Andrey
AU - Bragin, Eugeny
AU - Kazantsev, Alexey
AU - Donova, Marina
PY - 2021
DA - 2021/12/10 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 2554
IS - 12
VL - 9
PMID - 34946155
SN - 2076-2607
ER -
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@article{2021_Lobastova,
author = {Tatyana Lobastova and Victoria Fokina and Sergey Tarlachkov and Andrey Shutov and Eugeny Bragin and Alexey Kazantsev and Marina Donova},
title = {Steroid Metabolism in Thermophilic Actinobacterium Saccharopolyspora hirsuta VKM Ac-666T},
journal = {Microorganisms},
year = {2021},
volume = {9},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {dec},
url = {https://doi.org/10.3390%2Fmicroorganisms9122554},
number = {12},
pages = {2554},
doi = {10.3390/microorganisms9122554}
}
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Lobastova, Tatyana, et al. “Steroid Metabolism in Thermophilic Actinobacterium Saccharopolyspora hirsuta VKM Ac-666T.” Microorganisms, vol. 9, no. 12, Dec. 2021, p. 2554. https://doi.org/10.3390%2Fmicroorganisms9122554.
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