Open Access
Molecules, volume 26, issue 8, pages 2151
Impaired expression of cytoplasmic actins leads to chromosomal instability of mda-mb-231 basal-like mammary gland cancer cell line
Publication type: Journal Article
Publication date: 2021-04-08
PubMed ID:
33917969
Organic Chemistry
Drug Discovery
Physical and Theoretical Chemistry
Pharmaceutical Science
Molecular Medicine
Analytical Chemistry
Chemistry (miscellaneous)
Abstract
We have shown previously that two cytoplasmic actin isoforms play different roles in neoplastic cell transformation. Namely, β-cytoplasmic actin acts as a tumor suppressor, whereas γ-cytoplasmic actin enhances malignant features of tumor cells. The distinct participation of each cytoplasmic actin in the cell cycle driving was also observed. The goal of this study was to describe the diverse roles of cytoplasmic actins in the progression of chromosomal instability of MDA-MB-231 basal-like human carcinoma cell line. We performed traditional methods of chromosome visualization, as well as 3D-IF microscopy and western blotting for CENP-A detection/quantification, to investigate chromosome morphology. Downregulation of cytoplasmic actin isoforms alters the phenotype and karyotype of MDA-MB-231 breast cancer cells. Moreover, β-actin depletion leads to the progression of chromosomal instability with endoreduplication and aneuploidy increase. On the contrary, γ-actin downregulation results not only in reduced percentage of mitotic carcinoma cells, but leads to chromosome stability, reduced polyploidy, and aneuploidy.
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Frontiers Media S.A.
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Dugina V. et al. Impaired expression of cytoplasmic actins leads to chromosomal instability of mda-mb-231 basal-like mammary gland cancer cell line // Molecules. 2021. Vol. 26. No. 8. p. 2151.
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Dugina V., Shagieva G., Novikova M., Lavrushkina S., Sokova O., Kireev I., Kopnin P. Impaired expression of cytoplasmic actins leads to chromosomal instability of mda-mb-231 basal-like mammary gland cancer cell line // Molecules. 2021. Vol. 26. No. 8. p. 2151.
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TY - JOUR
DO - 10.3390/molecules26082151
UR - https://doi.org/10.3390%2Fmolecules26082151
TI - Impaired expression of cytoplasmic actins leads to chromosomal instability of mda-mb-231 basal-like mammary gland cancer cell line
T2 - Molecules
AU - Sokova, Olga
AU - Kireev, Igor
AU - Lavrushkina, Svetlana
AU - Dugina, Vera
AU - Novikova, Mariya
AU - Shagieva, Galina
AU - Kopnin, Pavel
PY - 2021
DA - 2021/04/08 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 2151
IS - 8
VL - 26
PMID - 33917969
SN - 1420-3049
ER -
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@article{2021_Dugina,
author = {Olga Sokova and Igor Kireev and Svetlana Lavrushkina and Vera Dugina and Mariya Novikova and Galina Shagieva and Pavel Kopnin},
title = {Impaired expression of cytoplasmic actins leads to chromosomal instability of mda-mb-231 basal-like mammary gland cancer cell line},
journal = {Molecules},
year = {2021},
volume = {26},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {apr},
url = {https://doi.org/10.3390%2Fmolecules26082151},
number = {8},
pages = {2151},
doi = {10.3390/molecules26082151}
}
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Dugina, Vera, et al. “Impaired expression of cytoplasmic actins leads to chromosomal instability of mda-mb-231 basal-like mammary gland cancer cell line.” Molecules, vol. 26, no. 8, Apr. 2021, p. 2151. https://doi.org/10.3390%2Fmolecules26082151.