Open Access

Pharmaceutics, volume 13, issue 8, pages 1145

Fluorescently Labeled PLGA Nanoparticles for Visualization In Vitro and In Vivo: The Importance of Dye Properties

Zhukova V ¹

Osipova Nadezhda ¹

Semyonkin Aleksey ¹

Malinovskaya Julia ¹

Melnikov Pavel A ²

Valikhov Marat ²

Porozov Yuri ^{3, 4}

Solovev Yaroslav ⁵

Kuliaev Pavel ⁶

Zhang Enqi ⁷

Sabel Bernhard A. ⁷

Chekhonin Vladimir ²

Abakumov Maxim A. ^{1, 8}

Majouga Alexander ¹

Kreuter Jörg ⁹

Henrich-Noack Petra ^{7, 10}

Gelperina S.E ¹

Maksimenko Olga ¹

Hide authors affiliations Show authors affiliations: 10 affiliations

Drug Delivery Systems Laboratory, D. Mendeleev University of Chemical Technology of Russia, Miusskaya pl. 9, 125047 Moscow, Russia |

Department of Neurobiology, V. Serbsky Federal Medical Research Centre of Psychiatry and Narcology of the Ministry of Health of the Russian Federation, Kropotkinskiy per. 23, 119034 Moscow, Russia |

Department of Computational Biology, Sirius University of Science and Technology, Olympic Ave 1, 354340 Sochi, Russia |

⁴

World-Class Research Center “Digital Biodesign and Personalized Healthcare”, I.M. Sechenov First Moscow State Medical University, Trubetskaya ul. 8, 119048 Moscow, Russia |

⁵

Laboratory of Bioinformatics Approaches in Combinatorial Chemistry and Biology, Department of Functioning of Living Systems, Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya ul. 16/10, 119991 Moscow, Russia |

⁶

TheoMAT Group, ITMO University, Kronverkskiy pr. 49, 197101 Saint Petersburg, Russia |

⁷

Institute of Medical Psychology, Otto-von-Guericke University, Leipziger Str. 44, 39120 Magdeburg, Germany |

⁸

Department of Medical Nanobiotecnology, Pirogov Russian National Research Medical University, Ostrovityanova ul 1, 117997 Moscow, Russia |

⁹

Institute of Pharmaceutical Technology, Biocenter, Goethe University, Max-von-Laue-Str. 9, 60438 Frankfurt am Main, Germany |

¹⁰

Clinic of Neurology with Institute of Translational Neurology, University Clinic Muenster, Mendel Str. 7, 48149 Muenster, Germany |

Publication type: Journal Article

Publication date: 2021-07-27

Multidisciplinary Digital Publishing Institute (MDPI)

Journal: Pharmaceutics

Quartile SCImago

Quartile WOS

Impact factor: 5.4

ISSN: 19994923

DOI: 10.3390/pharmaceutics13081145

Copy DOI

PubMed ID: 34452106

Pharmaceutical Science

Abstract

Fluorescently labeled nanoparticles are widely used for evaluating their distribution in the biological environment. However, dye leakage can lead to misinterpretations of the nanoparticles’ biodistribution. To better understand the interactions of dyes and nanoparticles and their biological environment, we explored PLGA nanoparticles labeled with four widely used dyes encapsulated (coumarin 6, rhodamine 123, DiI) or bound covalently to the polymer (Cy5.5.). The DiI label was stable in both aqueous and lipophilic environments, whereas the quick release of coumarin 6 was observed in model media containing albumin (42%) or liposomes (62%), which could be explained by the different affinity of these dyes to the polymer and lipophilic structures and which we also confirmed by computational modeling (log PDPPC/PLGA: DiI—2.3, Cou6—0.7). The importance of these factors was demonstrated by in vivo neuroimaging (ICON) of the rat retina using double-labeled Cy5.5/Cou6-nanoparticles: encapsulated Cou6 quickly leaked into the tissue, whereas the stably bound Cy.5.5 label remained associated with the vessels. This observation is a good example of the possible misinterpretation of imaging results because the coumarin 6 distribution creates the impression that nanoparticles effectively crossed the blood–retina barrier, whereas in fact no signal from the core material was found beyond the blood vessels.

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Citations by journals

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Pharmaceutics	Pharmaceutics, 2, 16.67% Pharmaceutics 2 publications, 16.67%
International Journal of Molecular Sciences	International Journal of Molecular Sciences, 2, 16.67% International Journal of Molecular Sciences 2 publications, 16.67%
Journal of Nanobiotechnology	Journal of Nanobiotechnology, 1, 8.33% Journal of Nanobiotechnology 1 publication, 8.33%
Advances in Colloid and Interface Science	Advances in Colloid and Interface Science, 1, 8.33% Advances in Colloid and Interface Science 1 publication, 8.33%
Journal of Drug Delivery Science and Technology	Journal of Drug Delivery Science and Technology, 1, 8.33% Journal of Drug Delivery Science and Technology 1 publication, 8.33%
ACS Applied Polymer Materials	ACS Applied Polymer Materials, 1, 8.33% ACS Applied Polymer Materials 1 publication, 8.33%
Mendeleev Communications	Mendeleev Communications, 1, 8.33% Mendeleev Communications 1 publication, 8.33%
Nature Reviews Methods Primers	Nature Reviews Methods Primers, 1, 8.33% Nature Reviews Methods Primers 1 publication, 8.33%
	1 2

Citations by publishers

	1 2 3 4
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 4, 33.33% Multidisciplinary Digital Publishing Institute (MDPI) 4 publications, 33.33%
Elsevier	Elsevier, 3, 25% Elsevier 3 publications, 25%
Springer Nature	Springer Nature, 2, 16.67% Springer Nature 2 publications, 16.67%
American Chemical Society (ACS)	American Chemical Society (ACS), 1, 8.33% American Chemical Society (ACS) 1 publication, 8.33%
	1 2 3 4

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Zhukova V. et al. Fluorescently Labeled PLGA Nanoparticles for Visualization In Vitro and In Vivo: The Importance of Dye Properties // Pharmaceutics. 2021. Vol. 13. No. 8. p. 1145.

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Zhukova V., Osipova N., Semyonkin A., Malinovskaya J., Melnikov P. A., Valikhov M., Porozov Y., Solovev Y., Kuliaev P., Zhang E., Sabel B. A., Chekhonin V., Abakumov M. A., Majouga A., Kreuter J., Henrich-Noack P., Gelperina S., Maksimenko O. Fluorescently Labeled PLGA Nanoparticles for Visualization In Vitro and In Vivo: The Importance of Dye Properties // Pharmaceutics. 2021. Vol. 13. No. 8. p. 1145.

RIS |

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RIS Copy

TY - JOUR

DO - 10.3390/pharmaceutics13081145

UR - https://doi.org/10.3390%2Fpharmaceutics13081145

TI - Fluorescently Labeled PLGA Nanoparticles for Visualization In Vitro and In Vivo: The Importance of Dye Properties

T2 - Pharmaceutics

AU - Osipova, Nadezhda

AU - Malinovskaya, Julia

AU - Melnikov, Pavel A

AU - Valikhov, Marat

AU - Solovev, Yaroslav

AU - Kuliaev, Pavel

AU - Zhang, Enqi

AU - Sabel, Bernhard A.

AU - Chekhonin, Vladimir

AU - Majouga, Alexander

AU - Kreuter, Jörg

AU - Henrich-Noack, Petra

AU - Maksimenko, Olga

AU - Zhukova, V

AU - Semyonkin, Aleksey

AU - Porozov, Yuri

AU - Abakumov, Maxim A.

AU - Gelperina, S.E

PY - 2021

DA - 2021/07/27 00:00:00

PB - Multidisciplinary Digital Publishing Institute (MDPI)

SP - 1145

IS - 8

VL - 13

PMID - 34452106

SN - 1999-4923

ER -

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@article{2021_Zhukova,

author = {Nadezhda Osipova and Julia Malinovskaya and Pavel A Melnikov and Marat Valikhov and Yaroslav Solovev and Pavel Kuliaev and Enqi Zhang and Bernhard A. Sabel and Vladimir Chekhonin and Alexander Majouga and Jörg Kreuter and Petra Henrich-Noack and Olga Maksimenko and V Zhukova and Aleksey Semyonkin and Yuri Porozov and Maxim A. Abakumov and S.E Gelperina},

title = {Fluorescently Labeled PLGA Nanoparticles for Visualization In Vitro and In Vivo: The Importance of Dye Properties},

journal = {Pharmaceutics},

year = {2021},

volume = {13},

publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},

month = {jul},

url = {https://doi.org/10.3390%2Fpharmaceutics13081145},

number = {8},

pages = {1145},

doi = {10.3390/pharmaceutics13081145}

}

MLA

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Zhukova, V., et al. “Fluorescently Labeled PLGA Nanoparticles for Visualization In Vitro and In Vivo: The Importance of Dye Properties.” Pharmaceutics, vol. 13, no. 8, Jul. 2021, p. 1145. https://doi.org/10.3390%2Fpharmaceutics13081145.

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Publisher

Multidisciplinary Digital Publishing Institute (MDPI)

Journal

Pharmaceutics

Quartile SCImago

Quartile WOS

Impact factor

5.4

ISSN

19994923 (Print)

Labs

Biomedical Nanomaterials Laboratory

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