Nematological Research (Japanese Journal of Nematology), volume 45, issue 1, pages 45-49
Protocol for root-knot nematode culture by a hydroponic system and nematode inoculation to Arabidopsis
Hidetaka Nishiyama
1
,
Ngan Bui Thi
1
,
Satoru Nakagami
1
,
Chica Ejima
1
,
Takashi Ishida
1
,
SHINICHIRO SAWA
1
Publication type: Journal Article
Publication date: 2015-01-01
SJR: —
CiteScore: —
Impact factor: —
ISSN: 09196765, 18823408
General Medicine
Abstract
Root-knot nematodes (Meloidogyne incognita) are one of the most economically damaging nematodes; thus it is important to know the molecular mechanisms involved in the phases of nematode infection.To use this nematode as a material for research, we must constantly prepare sufficient numbers of nematodes, preferably sterilized.Here we have developed alternative methods for culture, sterilization, and inoculation of root-knot nematodes that are especially suitable for the use of Arabidopsis thaliana Heynh as host plants.Nematol.Res. 45(1), 45-49(2015).
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